3.4.21.1: chymotrypsin

This is an abbreviated version!
For detailed information about chymotrypsin, go to the full flat file.

Reaction

Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/- =

Synonyms

4CHA, Alcalase, alpha chymar, alpha chymotrypsin, alpha-Chy, alpha-chymar ophth, alpha-chymotrypsin, alpha-chymotrypsin A, alpha-CT, avazyme, bovine alpha-chymotrypsin, caldecrin, cationic chymotrypsin, cellulomonadin, CHT, Chtp, Chtr1, Chtr2, Chtr3, Chtr4, ChTRP, CHY1, CHY20, chymar, chymotest, chymotrypsin, chymotrypsin A, chymotrypsin B, chymotrypsin C, chymotrypsin C1, chymotrypsin I, chymotrypsin II, chymotrypsin isoform Kh1, chymotrypsin isoform Kh2, chymotrypsin isoform Kh3, chymotrypsin-B, CTRA, Ctrb, EC 3.4.4.5, EC 3.4.4.6, enzeon, LBCP, lysosomal Bid cleavage protease, PEG-alpha-chymotrypsin, PEG-modified alpha-chymotrypsin, quimar, quimotrase, serine protease

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.1 chymotrypsin

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Results	in table
24	Activating Compound
103	AA Sequence
19	Application
1	CAS Registry Number
7	Cloned(Commentary)
14	Crystallization (Commentary)
1998	Disease/ Diagnostics
5	General Information
11	General Stability
45	IC50 Value
335	Inhibitors
44	kcat/KM [mM/s]
115	Ki Value [mM]
189	KM Value [mM]
5	Localization
11	Metals/Ions
35	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organic Solvent Stability
199	Organism
245	PDB ID
31	pH Optimum
16	pH Range
17	pH Stability
10	pI Value
8	Posttranslational Modification
5	Protein Variants
32	Purification (Commentary)
2	Reaction
1	Reaction Type
172	Reference
113	Source Tissue
33	Specific Activity [micromol/min/mg]
6	Storage Stability
250	Substrates and Products (Substrate)
19	Subunits
148	Synonyms
16	Temperature Optimum [°C]
11	Temperature Range [°C]
27	Temperature Stability [°C]
187	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.4.21.1 - chymotrypsin

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

0°C, stable for months

Gallus gallus

-

171864

25°C, free enzyme in 80 mM Tris-HCl at pH 7.8, 22 days, complete loss of activity

Bos taurus

-

681488

25°C, nanogel-immobilized enzyme, 36 days, about 10% loss of activity

Bos taurus

-

681488

25°C, tetrabutylammonium bromide, 2 months, 30% loss of activity

Bos taurus

-

651114

4°C, enzyme form LCTa is very susceptible to autolysis, enzyme form LCTb is more stable but undergoes considerable breakdown if stored for more than 3-4 days

Lucilia cuprina

-

171872

4°C, free enzyme in 80 mM Tris-HCl at pH 7.8, 36 days, 16.5% loss of activity

Bos taurus

-

681488