3.4.21.1: chymotrypsin

This is an abbreviated version!
For detailed information about chymotrypsin, go to the full flat file.

Word Map on EC 3.4.21.1

3.4.21.1

pancreatic

elastase

proteinase

pepsin

pancreas

subtilisin

lipase

amylase

thrombin

exocrine

cathepsin

porcine

soybean

pronase

aureus

chymotryptic

plasmin

staphylococcus

erythrocyte

kallikrein

carboxypeptidase

thermolysin

bromide

trypsin-like

juice

bowman-birk

duodenal

cyanogen

edman

kunitz-type

collagenase

kunitz

midguts

zymogen

neuraminidase

serpins

cholecystokinin

trypsinogen

secretin

2-macroglobulin

chymase

bromelain

aprotinin

chloromethyl

p-nitroanilide

medicine

synthesis

gizzard

biotechnology

meromyosin

non-cholinergic

amidolytic

drug development

acyl-enzyme

agriculture

industry

Reaction

Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/- =

Synonyms

4CHA, Alcalase, alpha chymar, alpha chymotrypsin, alpha-Chy, alpha-chymar ophth, alpha-chymotrypsin, alpha-chymotrypsin A, alpha-CT, avazyme, bovine alpha-chymotrypsin, caldecrin, cationic chymotrypsin, cellulomonadin, CHT, Chtp, Chtr1, Chtr2, Chtr3, Chtr4, ChTRP, CHY1, CHY20, chymar, chymotest, chymotrypsin, chymotrypsin A, chymotrypsin B, chymotrypsin C, chymotrypsin C1, chymotrypsin I, chymotrypsin II, chymotrypsin isoform Kh1, chymotrypsin isoform Kh2, chymotrypsin isoform Kh3, chymotrypsin-B, CTRA, Ctrb, EC 3.4.4.5, EC 3.4.4.6, enzeon, LBCP, lysosomal Bid cleavage protease, PEG-alpha-chymotrypsin, PEG-modified alpha-chymotrypsin, quimar, quimotrase, serine protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.1 chymotrypsin

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Results	in table
24	Activating Compound
103	AA Sequence
19	Application
1	CAS Registry Number
7	Cloned(Commentary)
14	Crystallization (Commentary)
1998	Disease/ Diagnostics
5	General Information
11	General Stability
45	IC50 Value
335	Inhibitors
44	kcat/KM [mM/s]
115	Ki Value [mM]
189	KM Value [mM]
5	Localization
11	Metals/Ions
35	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organic Solvent Stability
199	Organism
245	PDB ID
31	pH Optimum
16	pH Range
17	pH Stability
10	pI Value
8	Posttranslational Modification
5	Protein Variants
32	Purification (Commentary)
2	Reaction
1	Reaction Type
172	Reference
113	Source Tissue
33	Specific Activity [micromol/min/mg]
6	Storage Stability
250	Substrates and Products (Substrate)
19	Subunits
148	Synonyms
16	Temperature Optimum [°C]
11	Temperature Range [°C]
27	Temperature Stability [°C]
187	Turnover Number [1/s]

Purification

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Purification on EC 3.4.21.1 - chymotrypsin

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17 entries

4 insect chymotrypsins from 3 different insect orders (Dictyoptera, Coleoptera, and two Lepidoptera) are isolated using affinity chromatography

Tenebrio molitor

690637

affinity chromatography

Struthio camelus

691907

ammonium sulfate fractionation, Sephadex G-75 gel filtration, and DEAE-Sepharose column chromatography

Sardinops sagax

679953

anion exchange column, cation exchange column, affinity F2

Camelus dromedarius

653202

chymotrypsin A and B, to homogeneity by ammonium sulfate fractionation and gel filtration

Carassius auratus

708555

chymotrypsin I and II, by ionic exchange chromatography

gel filtration

gel filtration and ion exchange chromatography

Silurus glanis

667239

purification from an activated homogenate of bovine pancreas by adsorption onto nonsoluble alginate beads. Under all the experimental conditions, the enzyme has a positive net electrical charge, whereas alginate is negatively charged. After performing steps of washing and desorption, chymotrypsin is purified 9times with an enzyme recovery of 62%. The method allows purification with suitable values from a raw sample like pancreas homogenate without a previous clarification step

Bos taurus

732841

recombinant CHY1

Metarhizium anisopliae

Q9Y843

652084

SB-TI-Sepharose column chromatography

Rattus norvegicus

P07338

680927

SBTI-Sepharose column chromatography and HiPrep Sephacryl-100 column gel filtration

Rattus norvegicus

682709

use of Sepharose-immobilized denatured alpha-chymotrypsin for purification

2 entries