3.4.21.1: chymotrypsin
This is an abbreviated version!
For detailed information about chymotrypsin, go to the full flat file.
Word Map on EC 3.4.21.1
-
3.4.21.1
-
pancreatic
-
elastase
-
proteinase
-
pepsin
-
pancreas
-
subtilisin
-
lipase
-
amylase
-
thrombin
-
exocrine
-
cathepsin
-
porcine
-
soybean
-
pronase
-
aureus
-
chymotryptic
-
plasmin
-
staphylococcus
-
erythrocyte
-
kallikrein
-
carboxypeptidase
-
thermolysin
-
bromide
-
trypsin-like
-
juice
-
bowman-birk
-
duodenal
-
cyanogen
-
edman
-
kunitz-type
-
collagenase
-
kunitz
-
midguts
-
zymogen
-
neuraminidase
-
serpins
-
cholecystokinin
-
trypsinogen
-
secretin
-
2-macroglobulin
-
chymase
-
bromelain
-
aprotinin
-
chloromethyl
-
p-nitroanilide
-
medicine
-
synthesis
-
gizzard
-
biotechnology
-
meromyosin
-
non-cholinergic
-
amidolytic
-
drug development
-
acyl-enzyme
-
agriculture
-
industry
- 3.4.21.1
- pancreatic
- elastase
- proteinase
- pepsin
- pancreas
- subtilisin
- lipase
- amylase
- thrombin
-
exocrine
- cathepsin
- porcine
- soybean
- pronase
- aureus
-
chymotryptic
- plasmin
- staphylococcus
- erythrocyte
- kallikrein
- carboxypeptidase
- thermolysin
- bromide
-
trypsin-like
- juice
-
bowman-birk
- duodenal
-
cyanogen
-
edman
-
kunitz-type
- collagenase
-
kunitz
- midguts
- zymogen
- neuraminidase
- serpins
- cholecystokinin
- trypsinogen
- secretin
-
2-macroglobulin
- chymase
- bromelain
- aprotinin
-
chloromethyl
- p-nitroanilide
- medicine
- synthesis
- gizzard
- biotechnology
- meromyosin
-
non-cholinergic
-
amidolytic
- drug development
- acyl-enzyme
- agriculture
- industry
Reaction
Preferential cleavage: Tyr-/-, Trp-/-, Phe-/-, Leu-/- =
Synonyms
4CHA, Alcalase, alpha chymar, alpha chymotrypsin, alpha-Chy, alpha-chymar ophth, alpha-chymotrypsin, alpha-chymotrypsin A, alpha-CT, avazyme, bovine alpha-chymotrypsin, caldecrin, cationic chymotrypsin, cellulomonadin, CHT, Chtp, Chtr1, Chtr2, Chtr3, Chtr4, ChTRP, CHY1, CHY20, chymar, chymotest, chymotrypsin, chymotrypsin A, chymotrypsin B, chymotrypsin C, chymotrypsin C1, chymotrypsin I, chymotrypsin II, chymotrypsin isoform Kh1, chymotrypsin isoform Kh2, chymotrypsin isoform Kh3, chymotrypsin-B, CTRA, Ctrb, EC 3.4.4.5, EC 3.4.4.6, enzeon, LBCP, lysosomal Bid cleavage protease, PEG-alpha-chymotrypsin, PEG-modified alpha-chymotrypsin, quimar, quimotrase, serine protease
ECTree
3.4.21.1 chymotrypsinAdvanced search results
results (
results (KCat KM Value
KCat KM Value on EC 3.4.21.1 - chymotrypsin
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
16
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
34
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
3500
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
2000
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.021
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.033
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.048
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.056
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.088
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.144
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.143
4-nitrophenyl benzoate
presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.247
4-nitrophenyl benzoate
presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
0.345
4-nitrophenyl benzoate
presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.365
4-nitrophenyl benzoate
presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.583
4-nitrophenyl benzoate
presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
0.668
4-nitrophenyl benzoate
presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.23
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 15% dimethyl sulfoxide, pH 8.7, 25°C
0.254
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C
0.305
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C
220
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 7% dimethyl sulfoxide, pH 8.7, 25°C
0.48
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C
10
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C
2.74
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication
3.14
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
3.18
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
3.48
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
4.44
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
5.35
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
5.87
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
6.42
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
6.47
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
179
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
pH 8.0, 25°C
1900
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin A, at pH 7.5 and 37°C
6800
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin B, at pH 7.5 and 37°C
