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3.4.17.22: metallocarboxypeptidase D

This is an abbreviated version!
For detailed information about metallocarboxypeptidase D, go to the full flat file.

Word Map on EC 3.4.17.22

Reaction

releases C-terminal Arg and Lys from polypeptides =

Synonyms

Carboxypeptidase D, carboxypeptidase-D, CPD, CPD-N, DCPD, duck carboxypeptidase D, gp180, metallocarboxypeptidase-D, More, p170, silver, svr

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.22 metallocarboxypeptidase D

Cloned

Cloned on EC 3.4.17.22 - metallocarboxypeptidase D

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus expression of constructs containing either the 1A or the 1B form and one of three different sequences of domain 2 of CPD (wild type, mutants svr1, or svrpoi) in Sf9 cells
expressed by transient expression in a human kidney cell line and primary duck hepatocytes
expression in Sf9 cells, using a baculovirus expression system
-
human carboxypeptidase D (residues 32-1298) is cloned into the pTriExTM-7 expression vector that encodes an IgM secretion signal sequence and an N-terminal Strep-Tag II fusion protein. The CPD-pTrieX-7 construct ias transfected into mammalian HEK293F cells for transient expression, using polyethylenimine as transfection reagent
transfection of HEK-293F cell
wild-type enzyme and point mutants are expressed at high levels in Sf9 cells using the baculovirus expression system
-