3.4.17.12: carboxypeptidase M
This is an abbreviated version!
For detailed information about carboxypeptidase M, go to the full flat file.
Word Map on EC 3.4.17.12
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3.4.17.12
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pain
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women
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lymphocyte
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contralateral
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children
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postoperative
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prophylactic
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myelinolysis
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mastectomy
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pontine
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knee
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cyclophosphamide
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unilateral
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wave
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rehabilitation
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hyponatremia
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decision
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summation
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session
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flexion
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accelerometer
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myoelectrical
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chlorpheniramine
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preoperative
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arthroplasty
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noxious
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phytohemagglutinin
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3h-thymidine
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maleate
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pressor
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pain-free
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decision-making
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analgesia
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psychophysical
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actigraph
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pokeweed
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moderate-to-vigorous
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3h-tdr
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disomy
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myotomy
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cuff
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physiotherapy
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arthroscopic
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gastroparesis
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suprathreshold
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dysrhythmias
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breast-conserving
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medicine
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trapezius
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manometry
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resting-state
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diagnostics
- 3.4.17.12
- pain
- women
- lymphocyte
-
contralateral
- children
-
postoperative
-
prophylactic
- myelinolysis
-
mastectomy
- pontine
- knee
- cyclophosphamide
-
unilateral
-
wave
-
rehabilitation
- hyponatremia
-
decision
-
summation
-
session
-
flexion
-
accelerometer
-
myoelectrical
-
chlorpheniramine
-
preoperative
-
arthroplasty
-
noxious
- phytohemagglutinin
-
3h-thymidine
- maleate
-
pressor
-
pain-free
-
decision-making
-
analgesia
-
psychophysical
-
actigraph
-
pokeweed
-
moderate-to-vigorous
-
3h-tdr
-
disomy
-
myotomy
-
cuff
-
physiotherapy
-
arthroscopic
-
gastroparesis
-
suprathreshold
- dysrhythmias
-
breast-conserving
- medicine
-
trapezius
-
manometry
-
resting-state
- diagnostics
Reaction
cleavage of C-terminal arginine or lysine residues from polypeptides =
Synonyms
carboxypeptidase M, carboxypeptidase-M, CPM, M14006 (Merops-ID), More
ECTree
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Cloned
Cloned on EC 3.4.17.12 - carboxypeptidase M
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CPM cDNAs are cloned into pcDNA3 or pcDNA6 for expression in mammalian cells, to express N-terminal CFP- or YFP-tagged CPM pECFP-C1 and pEYFP-C1 vectors are used, to generate a fusion protein of CPM attached to the extracellular N-terminus of B1R pcDNA3 and 6 vectors are used
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DNA and amino acid sequence determination and analysis, genetic structure, localization on chromosome 12q13-q15, expression in insect cells via baculovirus transfection
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expressed in HEK-293 cells, in Pichia pastoris, and in baculovirus-infected insect cells
expression of 3'-truncated, glycosylphosphatidylinositol-free, soluble enzyme in insect cells using the baculovirus transfection system
expression of wild-type and mutant enzymes in COS-7 cells or HEK-293 cells. The wild-type and S406A and S406T mutants are expressed on the plasma membrane in glycosylphosphatidylinositol-anchored form, the S406P mutant is notz and is retained in a perinuclear location. Expression in baculovirus infected cells in a glycophosphatidyl-anchored form, whereas a truncated form, lacking the putative signal sequence for glycosylphosphatidyl anchoring is secreted at high levels into the medium. Both forms have lower molecular masses than native placental enzyme indicating a minimal glycosylation
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into the pCMV5, pGex4T-1 and pMalC2 vector for expression in COS-7 and Escherichia coli cells
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PCR-amplified fragment encoding hCPM is cloned into the pGEM-T-Easy cloning vector, and subsequent into the pPIC9 vector for transfomation of Pichia pastoris cells
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placental cDNA is incorporated in the pICZalphaA shuttle vector, signal peptide and C-terminal hydrophobic membrane anchor signal are removed