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3.4.17.1: carboxypeptidase A

This is an abbreviated version!
For detailed information about carboxypeptidase A, go to the full flat file.

Word Map on EC 3.4.17.1

Reaction

release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro =

Synonyms

A-type metallocarboxypeptidase, AcCP, Anson's enzyme, ASPA, aspartoacyclase, carboxypeptidase, carboxypeptidase a, carboxypeptidase A-6, carboxypeptidase A-like activity, carboxypeptidase A1, carboxypeptidase A2, Carboxypeptidase A3, carboxypeptidase A4, carboxypeptidase vitellogenic-like, carboxypeptidase-A, carboxypolypeptidase, colon mast cell carboxypeptidase, CPA, CPA1, CPA3, CPA4, CPA6, CPAI, CPD, CPVL, EC 3.4.12.2, EC 3.4.2.1, hCPA, hCPA4, mast cell carboxypeptidase A, mast cell CPA, mast cell-CPA, mast-cell carboxypeptidase A, mast-cell CPA, MC-CP, MC-CPA, MCCPA, MCP-2, mCPA, MDCP-A1, MDCP-A2, MeCPA, MF-CPA, molting carboxypeptidase A, molting fluid carboxypeptidase A, More, Nna1/CCP1, ochratoxin A hydrolytic enzyme, PTD012, RMC-CP, vitellogenic-like carboxypeptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.1 carboxypeptidase A

Purification

Purification on EC 3.4.17.1 - carboxypeptidase A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
also brain protein P30
-
also procarboxypeptidase
-
by anion-exchange chromatography
by gel filtration and on anion-exchange column
-
from pancreatin
-
further purification of commercial preparation by gel filtration in presence of Zn2+ and latexin
proteins were extracted from larvae and pupae of the insect, and adult flies
purified in one step using Co-NTA resin
purified with GST affinity chromatography
-
recombinant enzyme is purified by His-Bind resin column chromatography, endogenous enzyme is purified by CNBr-activated Sepharose column chromatography
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recombinant maltose binding protein-fusion enzyme from Escherichia coli by amylose affinity chromatography, digestion with thrombin, and heparin affinity chromatography
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recombinant pro-CPA1 from Saccharomyces cerevisiae by anion exchange and hydrophobic interaction chromatography and another step of anion exchange chromatography followed by dialsis and gel filtration, to homogeneity
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the active protein is obtained through tryptic activation, the resulting protein-product complex is subsequently purified by anion-exchange chromatography
the biotin-PEGylated carboxypeptidase complex is purified by gel filtration
-
the enzyme is purified from the culture medium by acetone precipitation and Macro-Prep High Q anion exchange chromatography
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to more than 80% purity
-
TSK DEAE 5PW column chromatography
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using a CABS-sepharose affinity column
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using Ni-NTA chromatography, ion exchange chromatography and gel filtration
-
using nickel affinity chromatography
-