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3.4.17.1: carboxypeptidase A

This is an abbreviated version!
For detailed information about carboxypeptidase A, go to the full flat file.

Word Map on EC 3.4.17.1

Reaction

release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro =

Synonyms

A-type metallocarboxypeptidase, AcCP, Anson's enzyme, ASPA, aspartoacyclase, carboxypeptidase, carboxypeptidase a, carboxypeptidase A-6, carboxypeptidase A-like activity, carboxypeptidase A1, carboxypeptidase A2, Carboxypeptidase A3, carboxypeptidase A4, carboxypeptidase vitellogenic-like, carboxypeptidase-A, carboxypolypeptidase, colon mast cell carboxypeptidase, CPA, CPA1, CPA3, CPA4, CPA6, CPAI, CPD, CPVL, EC 3.4.12.2, EC 3.4.2.1, hCPA, hCPA4, mast cell carboxypeptidase A, mast cell CPA, mast cell-CPA, mast-cell carboxypeptidase A, mast-cell CPA, MC-CP, MC-CPA, MCCPA, MCP-2, mCPA, MDCP-A1, MDCP-A2, MeCPA, MF-CPA, molting carboxypeptidase A, molting fluid carboxypeptidase A, More, Nna1/CCP1, ochratoxin A hydrolytic enzyme, PTD012, RMC-CP, vitellogenic-like carboxypeptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.1 carboxypeptidase A

Cloned

Cloned on EC 3.4.17.1 - carboxypeptidase A

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a cDNA library is constructed using the SMART cDNA construction kit, the PCR fragments are cloned directly into plasmid pCR2.1-TOPO
a gamma-SmscFv/hCPA fusion protein is constructed, the pGEX-4T-1 and pUC19 vectors are used, recombinant proteins are expressed in Escherichia coli DH5alpha cells
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a pBSK-based vector construct is used for gene targeting
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comparison of exon sequence composition of different carboxypeptidases
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DNA and amino acid sequence determination and analysis of midgut carboxypeptidase A
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DNA and amino acid sequence determination and analysis of the preproenzyme, phylogenetic analysis
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as maltose binding protein-fusion protein with low activity level, methanol-inducible expression in Pichia pastoris with a high activity of the secreted recombinant enzyme
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DNA and amino acid sequence determination and analysis, expression of the pro-CPA1 in Saccharomyces cerevisiae
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DNA and amino acid sequence determination and analysis, the gene contains 11 exons and is located on chromosome 3, genetic organization, phylogenetic analysis
expressed in Escherichia coli as a His-tagged fusion protein
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expressed in Escherichia coli BL21 (DE3) cells
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expressed in Escherichia coli XL1 Blue cells as catalytically active poly-His-tagged recombinant enzyme. GST-fusion proteins expressed in Escherichia coli BL21 Codon Plus (DE3) cells
expressed in Pichia pastoris
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expression in Escherichia coli
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expression of CPA4 in Pichia pastoris, scecretion of the recombinant enzyme to the culture medium
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expression of His-tagged pro-enzyme in Escherichia coli and in HEK293 cells also expressing the Epstein Barr virus nuclear antigen 1, induction by A23187
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four cDNA libraries are constructed using the SMART cDNA construction kit, the PCR fragments are cloned directly into a plasmid using a TOPO TA cloning kit
full-length human ASPA cDNA is subcloned into the pcDNA3.1/V5-His-TOPO vector, for bacterial expression mutants are generated directly in the pBAD/Thio-TOPO vector
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gene Cpa6, DNA and amino acid sequence determination and analysis
gene MF-CPA, phylogenetic analysis of the molting fluid carboxypeptidase A, expression analysis during development
hCPA4 is produced as a zymogen through recombinant heterologous overexpression using vector pPIC9 and the methylotrophic yeast Pichia pastoris
into vector pET-28a(+) and expressed in Escherichia coli BL21-DE3 cells. BALB/c mice inoculated subcutaneously with recombinant CPVL. CHO cells transiently transfected with CPVL-EGFP expression vector
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into vector pQTEV and introduced into Escherichia coli SCS1 cells carrying pRARE. The resulting clone used for expression of unlabeled protein. For expression of selenomethionine-labeled protein, the plasmid used to transform B834 Escherichia coli cells
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produced as a zymogen through recombinant heterologous overexpression using vector pPIC9 and the methylotrophic yeast Pichia pastoris as expression host
subcloned into pcDNA3 lacking the KpnI site. MYPYDVPDYA ligated into the KpnI site of CCP1, which is located in the C-terminal region following the carboxypeptidase domain. Expression in Neuro2A and NIH3T3 cells
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