3.4.15.5: Peptidyl-dipeptidase Dcp

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For detailed information about Peptidyl-dipeptidase Dcp, go to the full flat file.

Reaction

Hydrolysis of unblocked, C-terminal dipeptides from oligopeptides, with broad specificity. Does not hydrolyse bonds in which P1' is Pro, or both P1 and P1' are Gly =

Synonyms

DCP, Dipeptidyl carboxypeptidase, dipeptidyl carboxypeptidase II, dipeptidylcarboxypeptidase, EC 3.4.15.1, EC 3.4.15.3, K-26-DCP, LdDCP, More, peptidyl-dipeptidase K-26-DCP

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.15 Peptidyl-dipeptidases

                3.4.15.5 Peptidyl-dipeptidase Dcp

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Results	in table
3030	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
2	Crystallization (Commentary)
6	General Information
1	IC50 Value
20	Inhibitors
3	kcat/KM [mM/s]
5	Ki Value [mM]
11	KM Value [mM]
1	Localization
8	Metals/Ions
4	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
186	Organism
3	PDB ID
4	pH Optimum
2	pH Range
1	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
11	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
44	Substrates and Products (Substrate)
5	Subunits
13	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.4.15.5 - Peptidyl-dipeptidase Dcp

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified enzyme free or in complex with natural microbial tripeptide product K-26, hanging drop vapour diffusion method, mixing of 10 mg/ml protein in 50 mM Tris, pH 8.0, and 150 mM NaCl, with reservoir solution consisting of 0.2 M MgCl2, 0.1 M sodium cacodylate, pH 6.5, and 20% PEG 8000, and equilibration over reservoir solution, at 16°C for 2 days. For the complex with K-26, the protein is preincubated with 5 mM K-26 at room temperature for 1 h before crystallisation. X-ray diffraction structure determination and analysis at 1.80-1.75 A resolution

Astrosporangium hypotensionis

A0A1L1QK30

753554

crystallization in presence of a octapeptide inhibitor, sitting-drop vapor-diffusion procedure, 2.0 A resolution, indication of a ligand-dependent hinge movement mechanism

Escherichia coli

P24171

669857