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3.4.14.5: dipeptidyl-peptidase IV

This is an abbreviated version!
For detailed information about dipeptidyl-peptidase IV, go to the full flat file.

Word Map on EC 3.4.14.5

Reaction

release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline =

Synonyms

(GLP1)-degrading enzyme, ACT3, ADA binding protein, ADA-binding protein, ADABP, adenosine deaminase binding protein, Adenosine deaminase complexing protein, adenosine deaminase-binding protein, amino acyl-prolyl dipeptidyl aminopeptidase, aminopeptidase, glycylproline, Bile canaliculus domain-specific membrane glycoprotein, CD26, CD26 peptidase, CD26/dipeptidyl peptidase, CD26/DP IV, DAP IV, DapB, dapUm, dipeptidyl aminopeptidase IV, dipeptidyl dipeptidase IV, dipeptidyl peptidase 4, dipeptidyl peptidase 8, dipeptidyl peptidase 9, dipeptidyl peptidase IV, dipeptidyl peptidase-4, dipeptidyl peptidase-IV, dipeptidyl-aminopeptidase IV, dipeptidyl-peptidase 4, dipeptidyl-peptidase IV (CD26), dipeptidyl-peptide hydrolase, dipeptidylpeptidase 4a, dipeptidylpeptidase 4b, dipeptidylpeptidase IV, dipeptidylpeptidase IV/CD26, dipeptidylpeptidase-IV, DP IV, DP-IV, DPIV, DPP, DPP IV, DPP IV/CD26, DPP-4, DPP-IV, DPP4, DPP8, DPP9, DPPIV, DppIVA, DppIVB, glucagon-like peptide 1-degrading enzyme, Gly-Pro-naphthylamidase, glycoprotein GP110, glycylproline aminopeptidase, glycylproline-dipeptidyl-aminopeptidase, glycylprolyl aminopeptidase, glycylprolyl dipeptidylaminopeptidase, GP110 glycoprotein, h-DPPIV, hDPPIV, leukocyte antigen CD26, lymphocyte, antigen CD26, More, omega DPPIV, omega enzyme, omega gene product, Pep X, peptidase, dipeptidyl, IV, PepX, postproline dipeptidyl aminopeptidase IV, prolyl-dipeptidyl-aminopeptidase, sipeptidyl peptidase IV, T cell triggering molecule Tp103, T-cell activation antigen CD26, THAM, Thymocyte-activating molecule, TP103, type IV dipeptidyl aminopeptidase, WC10, X-PDAP, X-prolyl dipeptidyl aminopeptidase, X-prolyl-dipeptidyl aminopeptidase, Xaa-Pro-dipeptidyl-aminopeptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.14 Dipeptidyl-peptidases and tripeptidyl-peptidases
                3.4.14.5 dipeptidyl-peptidase IV

Purification

Purification on EC 3.4.14.5 - dipeptidyl-peptidase IV

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Sephacryl S-200 gel filtration
amylose column chromatography
DEAE Sepharose column chromatography Con A-Sepharose column chromatography, HiTrap nickel-chelating column chromatography, HiTrap phenyl column chromatography, ammonium sulfate precipitation, HiTrap Q column chromatography, Superdex 200 gel filtration, and Lys-isoindole affinity gel filtration
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from green barley malt, more than 43000fold
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from kidney cortex, 280fold
membrane bound form and soluble form
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native enzyme 1000fold to homogeneity, and recombinant enzyme from Escherichia coli
native from serum and seminal plasma, recombinant from K562 cell dulture medium
partial
partial, preparation of microsomal fraction
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recombinant enzyme from insect cells, rapidly to homogeneity
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recombinant His-tag fusion protein
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recombinant His-tagged soluble enzyme from Sf21 insect cell culture supernatant
recombinant soluble enzyme ectodomain from Pichia pastoris, to homogeneity
recombinant soluble enzyme from insect cell culture supernatant to homogeneity
recombinant soluble extracellular domain from cell culture supernatant
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recombinant soluble His-tagged extracellular part of the enzyme from Pichia pastoris culture medium, 534fold
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recombinant soluble His-tagged wild-type and mutant extracellular enzyme domains from insect cell culture supernatant to homogeneity
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recombinant soluble His-tagged wild-type and selenomethionine enzymes from insect cell culture supernatant to homogeneity
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Strep-Tactin resin purification system
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two enzyme forms: a plasma membrane form, mDPP, and a soluble form, sDPP
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