Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.13.22: D-Ala-D-Ala dipeptidase

This is an abbreviated version!
For detailed information about D-Ala-D-Ala dipeptidase, go to the full flat file.

Word Map on EC 3.4.13.22

Reaction

D-Ala-D-Ala
+
H2O
= 2 D-Ala

Synonyms

AAD, D-, D-dipeptidase, D-Ala-D-Ala amino dipeptidase, D-Ala-D-Ala dipeptidase, D-Ala-D-Ala dipeptidase VanX, D-Ala-D-Ala-dipeptidase, D-alanyl-D-alanine dipeptidase, M15.011, MAB1843, Vancomycin B-type resistance protein vanX, VanX, VanXY, VanXYc

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.13 Dipeptidases
                3.4.13.22 D-Ala-D-Ala dipeptidase

KI Value

KI Value on EC 3.4.13.22 - D-Ala-D-Ala dipeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
D-Ala-D-(2-difluorothio)glycine-OH
-
pH 7.0, 37°C
0.07
D-Ala-D-Ala
-
-
242
D-Ala-D-lactate
pH 7.0-9.0, 37°C
58
D-Ala-L-Ala
-
pH 8.0, 37°C
0.0165
D-Ala-PSI[P(OOH)O]-D-Ala
-
koff: 0.0180 sec-1, results reveals that both dipeptide phosphonates are slow-binding inhibitors of VanX
0.00196
D-Ala-PSI[P(OOH)O]-D-Phe
-
koff: 0.00231 sec-1, results reveals that both dipeptide phosphonates are slow-binding inhibitors of VanX. Moreover, in comparison with D-Ala(P,O)D-Ala phosphonate dipeptide, an additional aromatic interaction with the Phe79 residue in the active site of the enzyme may account for its higher affinity to VanX
0.0188
D-cysteine
-
pH 8.0, 37°C
225
L-Ala-D-Ala
-
pH 8.0, 37°C
98
L-Ala-L-Ala
-
pH 8.0, 37°C
additional information
additional information
-
inhibition kinetics, Ki is 0.0015 mM immediately after addition of the enzyme, but is then lowered to by a relatively slow isomerization step to a second complex to Ki = 0.00047 mM
-