Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.13.22: D-Ala-D-Ala dipeptidase

This is an abbreviated version!
For detailed information about D-Ala-D-Ala dipeptidase, go to the full flat file.

Word Map on EC 3.4.13.22

Reaction

D-Ala-D-Ala
+
H2O
= 2 D-Ala

Synonyms

AAD, D-, D-dipeptidase, D-Ala-D-Ala amino dipeptidase, D-Ala-D-Ala dipeptidase, D-Ala-D-Ala dipeptidase VanX, D-Ala-D-Ala-dipeptidase, D-alanyl-D-alanine dipeptidase, M15.011, MAB1843, Vancomycin B-type resistance protein vanX, VanX, VanXY, VanXYc

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.13 Dipeptidases
                3.4.13.22 D-Ala-D-Ala dipeptidase

Cloned

Cloned on EC 3.4.13.22 - D-Ala-D-Ala dipeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a maltose-binding protein fusion protein in Escherichia coli
expression in Escherichia coli and clinical strain VRE
-
gene vanX, genomic organization, functional expression of the soluble enzyme in Escherichia coli BL21(DE3)
gene vanX, overexpression of the soluble enzyme in Escherichia coli
overexpression in Escherichia coli
-
overexpression is achieved by cloning aad under the control of the nisin-inducible promoter PnisA from Lactococcus lactis. The resulting plasmid (pGIM023) is introduced into Lactobacillus plantarum strain NZ7100, a WCFS1 derivative carrying the nisR and nisK regulatory genes required for nisin induction
-
overexpression of wild-type and mutants as maltose-binding-protein fusion proteins
-