3.4.11.B4: Pyrococcus horikoshii deblocking aminopeptidase

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For detailed information about Pyrococcus horikoshii deblocking aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.B4

3.4.11.B4

hyperthermophilic

archaeon

furiosus

thermococcus

cobalt

leu-pna

tetrahedral

aminopeptidases

onnurineus

exoprotease

dodecameric

oligomer

Reaction

The enzyme exhibits aminopeptidase activity and with lower efficiency deblocking activity (i.e. hydrolysis of acetylated amino acids from the N-terminus of peptides). The turnover number with Ac-Ala-Ala-Ala is 260-540fold lower compared to the activity with Ala-Ala-Ala. The turnover number with Ac-Ala-Ala is 350fold lower compared to the activity with Ala-Ala. Low hydrolytic activity for peptide substrates longer than 10 residues. The enzyme is activated by Co2+ or Zn2+. Contains 1.32 mol of Ca2+ per mol of monomer. =

Synonyms

DAP1, DAP2, DAP3, DAPPh, DAPPh2, DAPPh3, deblocking aminopeptidase, PH0519, PH1527, PH1821

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.11 Aminopeptidases

3.4.11.B4 Pyrococcus horikoshii deblocking aminopeptidase

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Results	in table
3	AA Sequence
4	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
2	Inhibitors
8	KM Value [mM]
6	Metals/Ions
6	Molecular Weight [Da]
7	Organism
3	pH Optimum
3	pH Range
3	Protein Variants
4	Purification (Commentary)
1	Reaction
4	Reference
17	Substrates and Products (Substrate)
4	Subunits
19	Synonyms
4	Temperature Optimum [°C]
3	Temperature Range [°C]
4	Temperature Stability [°C]
9	Turnover Number [1/s]

Reference

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Reference on EC 3.4.11.B4 - Pyrococcus horikoshii deblocking aminopeptidase

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718484

Porciero, S.; Receveur-Br�chot, V.; Mori, K.; Franzetti, B.; Roussel, A.

Expression, purification, crystallization and preliminary crystallographic analysis of a deblocking aminopeptidase from Pyrococcus horikoshii

Acta Crystallogr. Sect. F

239-242

2005

Pyrococcus horikoshii (O58255), Pyrococcus horikoshii (O59196), Pyrococcus horikoshii (O59485), Pyrococcus horikoshii

16511005

718729

Onoe, S.; Ando, S.; Ataka, M.; Ishikawa, K.

Active site of deblocking aminopeptidase from Pyrococcus horikoshii

Biochem. Biophys. Res. Commun.

290

994-997

2002

Pyrococcus horikoshii (O58255), Pyrococcus horikoshii

11798173

719112

Mori, K.; Ishikawa, K.

New deblocking aminopeptidases from Pyrococcus horikoshii

Biosci. Biotechnol. Biochem.

1854-1860

2005

Pyrococcus horikoshii (O59196), Pyrococcus horikoshii (O59485)

16244434

719480

Ando, S.; Ishikawa, K.; Ishida, H.; Kawarabayasi, Y.; Kikuchi, H.; Kosugi, Y.

Thermostable aminopeptidase from Pyrococcus horikoshii

FEBS Lett.

447

25-28

1999

Pyrococcus horikoshii (O58255), Pyrococcus horikoshii

10218575