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0.515 - 2.307
alpha-L-Asp-2-naphthylamide
0.243 - 2.687
alpha-L-Glu-2-naphthylamide
0.1 - 5.94
alpha-L-Glu-beta-naphthylamide
0.0391 - 12.5
alpha-L-glutamyl-beta-naphthylamide
0.062 - 2.85
angiotensin II
1.4 - 2.3
Asp-4-methylcoumaryl-7-amide
0.3273 - 0.3848
Asp-7-amido-4-methylcoumarin
0.72 - 0.846
Asp-beta-naphthylamide
-
maternal and normal serum
1.21 - 3.25
cholecystokinin-8
0.6 - 7.1
Gln-4-methylcoumaryl-7-amide
0.4 - 1.2
Glu-4-methylcoumaryl-7-amide
0.1351 - 4.36
Glu-7-amido-4-methylcoumarin
0.031 - 1.2
Glu-beta-naphthylamide
0.87 - 2.05
Glu-p-nitroanilide
0.34 - 1.21
L-Asp-4-nitroanilide
0.159 - 2.034
L-Glu-2-naphthylamide
0.19 - 25.2
L-Glu-4-nitroanilide
0.152 - 1.475
L-Glu-p-nitroanilide
additional information
additional information
-
0.515
alpha-L-Asp-2-naphthylamide
pH 7.4, 37°C, wild-type enzyme
2.272
alpha-L-Asp-2-naphthylamide
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
2.307
alpha-L-Asp-2-naphthylamide
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
0.243
alpha-L-Glu-2-naphthylamide
pH 7.4, 37°C, wild-type enzyme
1.943
alpha-L-Glu-2-naphthylamide
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
2.687
alpha-L-Glu-2-naphthylamide
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
0.1
alpha-L-Glu-beta-naphthylamide
pH 7.4, 25°C
0.16
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, wild-type enzyme
0.171
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, wild-type enzyme, in presence of 4 mM Ca2+
0.432
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, wild-type enzyme, in presence of 0.025 mM Ca2+
0.697
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, wild-type enzyme, without Ca2+
0.87
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, mutant enzyme E215Q
1.5
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, mutant enzyme E215D
2.8
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, mutant enzyme E215A
4.383
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, mutant enzyme H450F enzyme, in presence of 4 mM Ca2+
5.94
alpha-L-Glu-beta-naphthylamide
pH 7.4, 37°C, mutant enzyme H450F, in presence of 0.025 mM Ca2+
0.0391
alpha-L-glutamyl-beta-naphthylamide
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme, with 4 mM Ca2+
0.165
alpha-L-glutamyl-beta-naphthylamide
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme
0.221
alpha-L-glutamyl-beta-naphthylamide
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A, with 4 mM Ca2+
0.269
alpha-L-glutamyl-beta-naphthylamide
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K, with 4 mM Ca2+
1.5
alpha-L-glutamyl-beta-naphthylamide
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A
12.5
alpha-L-glutamyl-beta-naphthylamide
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K
0.062
angiotensin II
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
0.073
angiotensin II
pH 7.4, 37°C, wild-type enzyme
0.161
angiotensin II
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
0.84
angiotensin II
-
wild-type, in the presence of 1.0 mM CaCl2
1.18
angiotensin II
-
mutant D221N, in the absence of CaCl2
1.22
angiotensin II
-
mutant D221N, in the presence of 1.0 mM CaCl2
2.85
angiotensin II
-
wild-type, in the absence of CaCl2
1.4
Asp-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
2.3
Asp-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
0.3273
Asp-7-amido-4-methylcoumarin
in the presence of 1 mM cobalt
0.3848
Asp-7-amido-4-methylcoumarin
in the absence of cobalt
0.21
Asp-Ala
-
-
1.21
cholecystokinin-8
-
wild-type, in the presence of 1.0 mM CaCl2
2.9
cholecystokinin-8
-
mutant D221N, in the presence of 1.0 mM CaCl2
2.93
cholecystokinin-8
-
mutant D221N, in the absence of CaCl2
3.25
cholecystokinin-8
-
wild-type, in the absence of CaCl2
0.6
Gln-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
7.1
Gln-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
0.4
Glu-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
1.2
Glu-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
0.1351
Glu-7-amido-4-methylcoumarin
in the absence of cobalt
0.1363
Glu-7-amido-4-methylcoumarin
in the presence of 1 mM cobalt
0.5
Glu-7-amido-4-methylcoumarin
-
wild-type, in the presence of 1.0 mM CaCl2
1.54
Glu-7-amido-4-methylcoumarin
-
mutant D221N, in the absence of CaCl2
1.65
Glu-7-amido-4-methylcoumarin
-
mutant D221N, in the presence of 1.0 mM CaCl2
1.67
Glu-7-amido-4-methylcoumarin
-
mutant D221Q, in the absence of CaCl2
1.84
Glu-7-amido-4-methylcoumarin
-
wild-type, in the absence of CaCl2
1.95
Glu-7-amido-4-methylcoumarin
-
mutant D221Q, in the presence of 1.0 mM CaCl2
2.87
Glu-7-amido-4-methylcoumarin
-
mutant D221E, in the presence of 1.0 mM CaCl2
3.61
Glu-7-amido-4-methylcoumarin
-
mutant D221E, in the absence of CaCl2
4.31
Glu-7-amido-4-methylcoumarin
-
mutant D221A, in the presence of 1.0 mM CaCl2
4.36
Glu-7-amido-4-methylcoumarin
-
mutant D221A, in the absence of CaCl2
0.28
Glu-Ala
-
-
0.031
Glu-beta-naphthylamide
-
-
0.091 - 1.2
Glu-beta-naphthylamide
-
value depending on source of enzyme and presence of divalent cations
0.091 - 1.2
Glu-beta-naphthylamide
-
value depending on source of enzyme and presence of divalent cations
0.87
Glu-p-nitroanilide
-
ATA II
2.05
Glu-p-nitroanilide
-
ATA I
0.36
kallidin
-
wild-type, in the absence of CaCl2
0.37
kallidin
-
mutant D221N, in the absence of CaCl2
0.6
kallidin
-
mutant D221N, in the presence of 1.0 mM CaCl2
1.13
kallidin
-
wild-type, in the presence of 1.0 mM CaCl2
0.34
L-Asp-4-nitroanilide
-
pH 6.0, 60°C, recombinant enzyme
1.21
L-Asp-4-nitroanilide
pH 6.0, 60°C, recombinant enzyme
0.159
L-Glu-2-naphthylamide
recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.198
L-Glu-2-naphthylamide
recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.258
L-Glu-2-naphthylamide
recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.282
L-Glu-2-naphthylamide
recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
1.136
L-Glu-2-naphthylamide
recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
1.481
L-Glu-2-naphthylamide
recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
1.919
L-Glu-2-naphthylamide
recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
2.034
L-Glu-2-naphthylamide
recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.19
L-Glu-4-nitroanilide
-
pH 6.0, 60°C, recombinant enzyme
25.2
L-Glu-4-nitroanilide
pH 6.0, 60°C, recombinant enzyme
0.152
L-Glu-p-nitroanilide
wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C
1.475
L-Glu-p-nitroanilide
wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C
1.84
neurokinin B
-
wild-type, in the presence of 1.0 mM CaCl2
3.97
neurokinin B
-
mutant D221N, in the absence of CaCl2
4.3
neurokinin B
-
mutant D221N, in the presence of 1.0 mM CaCl2
4.94
neurokinin B
-
wild-type, in the absence of CaCl2
additional information
additional information
kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics and substrate specificity in presence or absence of Ca2+
-