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0.0044
(1-aminopropane-1,3-diyl)bis(phosphonic acid)
-
0.00034
(3R)-3-amino-3-phosphonopropanoic acid
-
0.000314 - 0.01
(4R)-4-amino-5-sulfanylpentanoic acid
0.000268 - 0.002
(S)-3-amino-4-mercapto-butyl sulfonic acid
0.00025
(S)-3-amino-4-mercaptobutyl sulfonic acid
-
-
0.000118 - 0.0139
2,6-diaminohexane-1-thiol
2
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid
-
0.0427
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid
-
0.1831
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid
-
0.000074 - 0.0065
4-amino-4-phosphonobutyric acid
0.105
5-amino-5-phosphonopentanoic acid
-
0.0000009
Ala-PSI[PO2-CH2]-Leu-Ala
pH 7.4, 25°C
0.0000059 - 0.0001927
amastatin
0.0271
amino(phosphono)acetic acid
-
0.395
amino[hydroxy(methyl)phosphoryl]acetic acid
-
0.00026 - 0.001297
angiotensin IV
0.0141 - 0.0289
captopril
0.0019 - 0.016
D-glutamate phosphonic acid
0.000194
Glu-PSI[PO2-CH2]-Leu
pH 7.4, 25°C
0.0000008
Glu-PSI[PO2-CH2]-Leu-Ala
pH 7.4, 25°C
0.00003
Glu-PSI[PO2-CH2]-Leu-Arg
pH 7.4, 25°C
0.0000057
Glu-PSI[PO2-CH2]-Leu-Asp
pH 7.4, 25°C
0.000037
Glu-PSI[PO2-CH2]-Leu-Leu
pH 7.4, 25°C
0.000038 - 0.056
glutamate phosphonic acid
0.00038 - 0.00206
glutamate thiol
0.00012 - 0.01
glutamate-thiol
0.000462 - 0.00202
glutamine-thiol
0.000045 - 0.021
L-glutamate phosphonic acid
0.0001 - 0.00394
lysine thiol
0.00046 - 0.0199
lysine-thiol
0.00019 - 0.0084
methionine thiol
0.000475 - 0.00918
methionine-thiol
0.00007
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
wild type enzyme, in 50 mM Tris/HCl buffer (pH 7.4), 4 mM Ca2+, at 37°C
0.00025
[(S)-3-amino-4-mercaptobutyl sulfonic acid]
-
-
0.000292
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
pH 7.3, 37°C
0.0000833
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
pH 7.3, 37°C
0.000053 - 0.00047
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
0.000283
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
pH 7.3, 37°C
0.0042
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
pH 7.3, 37°C
0.0012
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
pH 7.3, 37°C
0.000027
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
pH 7.3, 37°C
0.0000733
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
pH 7.3, 37°C
0.000267
[[(2R,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.00091
[[(2R,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.00005
[[(2R,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.000016
[[(2R,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.000051
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentane]-Ile-Asp-OH
pH 7.3, 37°C
0.00008
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.00015
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Tyr-Asp-OH
pH 7.3, 37°C
0.0000147
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
pH 7.3, 37°C
0.0000036
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
pH 7.3, 37°C
0.0000032 - 0.000015
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
0.00000536
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
pH 7.3, 37°C
0.000145
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
pH 7.3, 37°C
0.00004
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
pH 7.3, 37°C
0.0000043
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
pH 7.3, 37°C
0.0000093
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
pH 7.3, 37°C
0.00000356
[[(2S,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.000012
[[(2S,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.00002
[[(2S,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.000013
[[(2S,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
additional information
additional information
-
inhibition constant of 3-amino-4-thio-butyl sulfonic acid for aminopeptidase A: 0.29 microM/L
-
0.000314
(4R)-4-amino-5-sulfanylpentanoic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme, with 4 mM Ca2+
0.00157
(4R)-4-amino-5-sulfanylpentanoic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme
0.00172
(4R)-4-amino-5-sulfanylpentanoic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A
0.01
(4R)-4-amino-5-sulfanylpentanoic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K
0.000268
(S)-3-amino-4-mercapto-butyl sulfonic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme, with 4 mM Ca2+
0.00152
(S)-3-amino-4-mercapto-butyl sulfonic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K, with 4 mM Ca2+
0.002
(S)-3-amino-4-mercapto-butyl sulfonic acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A, with 4 mM Ca2+
0.000118
2,6-diaminohexane-1-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A
0.000317
2,6-diaminohexane-1-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme
0.000378
2,6-diaminohexane-1-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K
0.00751
2,6-diaminohexane-1-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A, with 4 mM Ca2+
0.0134
2,6-diaminohexane-1-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme, with 4 mM Ca2+
0.0139
2,6-diaminohexane-1-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K, with 4 mM Ca2+
0.000074
4-amino-4-phosphonobutyric acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme, with 4 mM Ca2+
0.000619
4-amino-4-phosphonobutyric acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K, with 4 mM Ca2+
0.0065
4-amino-4-phosphonobutyric acid
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A, with 4 mM Ca2+
0.0000059
amastatin
-
mutant D221, in the presence of 1 mM CaCl2
0.0000177
amastatin
-
mutant D221, in the absence of CaCl2
0.0000243
amastatin
-
wild-type enzyme, in the absence of CaCl2
0.0001927
amastatin
-
wild-type enzyme, in the presence of 1 mM CaCl2
0.00026
angiotensin IV
-
mutant D221, in the absence of CaCl2
0.000324
angiotensin IV
-
mutant D221, in the presence of 1 mM CaCl2
0.000386
angiotensin IV
-
wild-type enzyme, in the absence of CaCl2
0.001297
angiotensin IV
-
wild-type enzyme, in the presence of 1 mM CaCl2
0.0141
captopril
pH 7.4, 37°C, mutant enzyme N363Q
0.0248
captopril
pH 7.4, 37°C, mutant enzyme N363A
0.0289
captopril
pH 7.4, 37°C, wild-type enzyme
0.0019
D-glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme
0.00331
D-glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme
0.00489
D-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme N363Q
0.005
D-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme E215D
0.011
D-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme E215A
0.0112
D-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme N363A
0.016
D-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme E215Q
0.000038
glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+
0.00061
glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+
0.00084
glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+
0.00287
glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme, no Ca2+
0.056
glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+
0.00038
glutamate thiol
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00085
glutamate thiol
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00103
glutamate thiol
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.0011
glutamate thiol
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00194
glutamate thiol
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00197
glutamate thiol
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.002
glutamate thiol
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00206
glutamate thiol
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00012
glutamate-thiol
pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+
0.000147
glutamate-thiol
pH 7.4, 37°C, wild-type enzyme
0.000216
glutamate-thiol
pH 7.4, 37°C, wild-type enzyme
0.00034
glutamate-thiol
pH 7.4, 37°C, mutant enzyme E215Q
0.00036
glutamate-thiol
pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+
0.00137
glutamate-thiol
pH 7.4, 37°C, mutant enzyme N363Q
0.00167
glutamate-thiol
pH 7.4, 37°C, wild-type enzyme, no Ca2+
0.002
glutamate-thiol
pH 7.4, 37°C, mutant enzyme N363A
0.00227
glutamate-thiol
pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+
0.00524
glutamate-thiol
pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+
0.0072
glutamate-thiol
pH 7.4, 37°C, mutant enzyme E215D
0.01
glutamate-thiol
pH 7.4, 37°C, mutant enzyme E215A
0.000462
glutamine-thiol
pH 7.4, 37°C, wild-type enzyme
0.00199
glutamine-thiol
pH 7.4, 37°C, mutant enzyme N363A
0.00202
glutamine-thiol
pH 7.4, 37°C, mutant enzyme N363Q
0.000045
L-glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme
0.00006
L-glutamate phosphonic acid
pH 7.4, 37°C, wild-type enzyme
0.000214
L-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme N363Q
0.00026
L-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme E215Q
0.000395
L-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme N363A
0.012
L-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme E215D
0.021
L-glutamate phosphonic acid
pH 7.4, 37°C, mutant enzyme E215A
0.0001
lysine thiol
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00022
lysine thiol
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00026
lysine thiol
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.0009
lysine thiol
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00275
lysine thiol
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00298
lysine thiol
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.0036
lysine thiol
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00394
lysine thiol
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00046
lysine-thiol
pH 7.4, 37°C, wild-type enzyme, Ca2+
0.00047
lysine-thiol
pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+
0.0012
lysine-thiol
pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+
0.0072
lysine-thiol
pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+
0.0199
lysine-thiol
pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+
0.00019
methionine thiol
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00026
methionine thiol
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00029
methionine thiol
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00058
methionine thiol
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00307
methionine thiol
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00403
methionine thiol
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00667
methionine thiol
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.0084
methionine thiol
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.000475
methionine-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A
0.00075
methionine-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme
0.00125
methionine-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K
0.00322
methionine-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878A
0.00514
methionine-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged mutant R878K
0.00918
methionine-thiol
pH 7.4, temperature not specified in the publication, recombinant His-tagged wild-type enzyme, with 4 mM Ca2+
0.000053
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.00047
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.0000032
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C
0.000015
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
pH 7.3, 37°C