Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.11.6: aminopeptidase B

This is an abbreviated version!
For detailed information about aminopeptidase B, go to the full flat file.

Word Map on EC 3.4.11.6

Reaction

release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys =

Synonyms

AAP, ACE2, Aminopeptidase, aminopeptidase B, aminopeptidase, arginine, aminopeptidase-B, Ap-B, APB, Arg-AP, Arg/Lys aminopeptidase, ArgAP, arginine aminopeptidase, arginine-aminopeptidase, arginyl aminopeptidase, arginyl peptidase, arylamidase, arylamidase II, BSAP, chloride-dependent-basic aminopeptidase, Cl--activated arginine aminopeptidase, Cl--activated arinine aminopeptidase, Co(II)-Ap-B, ColAP, cold-active aminopeptidase, Cu(II)-Ap-B, cytosol aminopeptidase, cytosol aminopeptidase IV, DAP BII, dipeptidyl aminopeptidase BII, double-zinc aminopeptidase, EC 3.4.13.6, KAP, L-arginine aminopeptidase, L-RAP, LAPase, leukocyte-derived arginine aminopeptidase, LRAP, lysine-specific aminopeptidase, minopeptidase, More, protease IV, pumAPE, RAP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.6 aminopeptidase B

Purification

Purification on EC 3.4.11.6 - aminopeptidase B

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms
279.5fold after heat inactivation, ammonium sulfate fractionation and column chromatographies
-
80.9fold to homogeneity by gel filtration, anion exchange chromatography, and again gel filtration
-
DEAE Toyopearl column chromatography and TALON column chromatography
expressed in Escherichia coli
-
glutathione-Sepharose 4B column chromatography and Q-Sepharose column chromatography
-
glutathione-Sepharose 4B column chromatography and Sephadex G-150 gel filtration
-
His-Trap column chromatography and Superdex S200 gel filtration
-
native enzyme 159000fold by ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography and gel filtration
-
native enzyme 460fold to homogeneity by ion exchange and hydroxy apatite chromatography, and ion exchange chromatography
-
native enzyme 69.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity
-
native enzyme from testis
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and DEAE Trisacryl Plus M column chromatography
-
partially, preparation of spores and subcellular fractionation
-
partially, subcellular fractionation
-
recombinant enzyme 5fold from Escherichia coli by ion exchange chromatography
Toyopearl DEAE-650 column chromatography, Mono Q column chromatography, and DEAE-650 column chromatography
-
using a glutathione-Sepharose 4B column, cleaving of the fusion-enzyme by thrombin, using a benzamidine-Sepharose and a Q-Sepharose column
-