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3.4.11.5: prolyl aminopeptidase

This is an abbreviated version!
For detailed information about prolyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.5

Reaction

release of N-terminal proline from a peptide =

Synonyms

aminopeptidase P, aminopeptidase, proline, APP, cytosol aminopeptidase V, EC 3.4.1.4, L-proline aminopeptidase, L-prolyl-peptide hydrolase, More, pamA, PAP, PchPiPA, PepI, pepIP, PEPP, Pf S33 proline aminopeptidase, PfPAP, PIP, PiPA, Plasmodium falciparum S33 proline aminopeptidase, Pro-X aminopeptidase, proline aminopeptidase, proline iminopeptidase, proline-specific aminopeptidase, Prolyl aminopeptidase, TaPAP1, TePAP, Tricorn protease interacting factor F1, TsPAP1, X-pro aminopeptidase (Lactococcus)

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.5 prolyl aminopeptidase

Specific Activity

Specific Activity on EC 3.4.11.5 - prolyl aminopeptidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.012
-
crude extract, in 50 mM Tris-HCl, pH 7.5, at 37°C
0.0215
-
culture supernatant
0.48
-
type III
0.8
Pro-2-naphthylamide, at 37°C, pH 8.0
1.06
-
purified enzyme
1.43
-
1 mM Pro-p-nitranilide in 0.05 M Tris/HCl, pH 7.5
10
-
after 700fold purification, in 50 mM Tris-HCl, pH 7.5, at 37°C
143.94
recombinant His-tagged enzyme purified from Pichia pastoris culture supernatant, pH 7.5, 50°C
166.52
recombinant His-tagged enzyme purified from Pichia pastoris cell lysate, pH 7.5, 50°C
18
-
after 835fold purification
18.3
substrate: L-Pro-4-nitroanilide
191
Lyophyllum cinerascens
-
-
2.4
substrate: PLSRTLSVAAKK
20.3
substrate: Pro-2-naphthylamide
22.2
substrate: Pro-Leu-Gly
247.3
purified recombinant enzyme, pH 7.5, 50°C
25.7
substrate: Pro-Ala
26.4
purified native enzyme, pH 7.4, 37°C
290
Pro-4-nitroanilide, four-fold purified enzyme at 30°C, pH 7.5
3.28
-
pH 7.0, 30°C
33.4
Pro-Leu-Gly-NH2, at 30°C, pH 7.5
4.5
substrate: PPGFSPFR
42.4
substrate: L-hydroxyproline-2-naphthylamide
5.13
-
Pro-p-nitroanilide
55.5
PPGFSPFR, at 30°C, pH 7.5
6.6
PLSRTLSVAAKK, at 30°C, pH 7.5
68.3
-
Pro-p-nitroanilide
780
-
Pro-Leu-NH2
8.34
4-acetyloxyproline-2-naphthylamide, at 37°C, pH 8.0
8.5
-
pH 7.5, 37°C
85.5
Pro-Ala, at 30°C, pH 7.5
additional information