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3.4.11.5: prolyl aminopeptidase

This is an abbreviated version!
For detailed information about prolyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.5

Reaction

release of N-terminal proline from a peptide =

Synonyms

aminopeptidase P, aminopeptidase, proline, APP, cytosol aminopeptidase V, EC 3.4.1.4, L-proline aminopeptidase, L-prolyl-peptide hydrolase, More, pamA, PAP, PchPiPA, PepI, pepIP, PEPP, Pf S33 proline aminopeptidase, PfPAP, PIP, PiPA, Plasmodium falciparum S33 proline aminopeptidase, Pro-X aminopeptidase, proline aminopeptidase, proline iminopeptidase, proline-specific aminopeptidase, Prolyl aminopeptidase, TaPAP1, TePAP, Tricorn protease interacting factor F1, TsPAP1, X-pro aminopeptidase (Lactococcus)

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.5 prolyl aminopeptidase

Purification

Purification on EC 3.4.11.5 - prolyl aminopeptidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 enzymes, II and III specific for naphthylamides of L-Pro and L-Hyp
oral microorganisms
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ammonium sulfate fractionation, Toyopearl QAE-550C column chromatography, Sephadex G-200 gel filtration, and Mono Q column chromatography
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chromatography
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column chromatography
DEAE Sepharose CL6B, Butyl-Toyopearl, Sephacryl S-100 and Mono-Q column chromatographies
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enzyme is partially purified through two-step column chromatography using a Ni-IMAC column and a MonoQ column
expressed in Escherichia coli
four chromatography steps
membrane bound enzyme 7.13fold by cation exchange chromatography, gel filtration, and anion exchange chromatography
partial
-
partial, 3 enzymes: I, II, III
-
purified to homogeneity by ammonium sulfate fractionation followed by five successive chromatographic steps
recombinant enzyme from Bacillus subtilis strain WB600 8.8fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
recombinant His-tagged enzyme 4.93fold from culture supernatant and 13.38fold from cell lysate of expressing Pichia pastoris strain GS115 by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminal His6-tagged PAP 4.3fold from Bacillus subtilis by dialysis, nickel affinity chromatography, and ultrafiltration
recombinant wild-type and mutant enzymes from Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
using Ni-NTA chromatography