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3.4.11.5: prolyl aminopeptidase

This is an abbreviated version!
For detailed information about prolyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.5

Reaction

release of N-terminal proline from a peptide =

Synonyms

aminopeptidase P, aminopeptidase, proline, APP, cytosol aminopeptidase V, EC 3.4.1.4, L-proline aminopeptidase, L-prolyl-peptide hydrolase, More, pamA, PAP, PchPiPA, PepI, pepIP, PEPP, Pf S33 proline aminopeptidase, PfPAP, PIP, PiPA, Plasmodium falciparum S33 proline aminopeptidase, Pro-X aminopeptidase, proline aminopeptidase, proline iminopeptidase, proline-specific aminopeptidase, Prolyl aminopeptidase, TaPAP1, TePAP, Tricorn protease interacting factor F1, TsPAP1, X-pro aminopeptidase (Lactococcus)

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.5 prolyl aminopeptidase

Cloned

Cloned on EC 3.4.11.5 - prolyl aminopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 as a His-tagged fusion protein
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expressed in Lactobacillus casei strain. The strain successfully survives 12 weeks of ripening period in cheese. The food-grade plasmid carrying the pepI gene, is stable and PepI enzyme is active in LAB6 cells isolated at different stages of the ripening process
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expression in Escherichia coli
expression in Escherichia coli. Enzyme carrying a C-terminal His-tag is less stable than native enzyme
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expression of wild-type and mutants in Escherichia coli
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gene pap, codon optimization and synthesis of the modified gene, optimized expression of N-terminally His-tagged prolyl aminopeptidase in Pichia pastoris strain GS115, subcloning in Escherichia coli strain JM109. The N-terminal His-tag has no effect on PAP expression, whereas a C-terminal His-tag inactivates PAP
gene pap, recombinant expression of His-tagged Pap in Bacillus subtilis strain WB600, for cell culture, batch fermentation conditions, including the agitation speed, pH and temperature, are systematically optimized based on a kinetic analysis, method optimization, overview. The yield of PAP reaches 174.8 U/ml under the optimized conditions, which is 1.66 times higher than that of the original production
gene pap, recombinant expression of N-terminal His6-tagged enzyme in Bacillus subtilis strain WB600, the enzyme is secreted by adding 2 mM CaCl2 and 5% D-sorbitol, extracellular and intracellular PAP activities are 7.2 and 78.6 U/ml, respectively. Sorbitol and mannitol raise the level of the secretion, but sorbitol is slightly better than mannitol and increases 100.19% compared with the control. CaCl2 can further improve the secretion level of the extracellular enzyme
gene pap, recombinant overexpression in Bacillus subtilis strain WB600 from plasmid pMA5-pap, about 5fold induction with 2 mM CaCl2 and 5% D-sorbitol in TB medium
gene pap1, recombinant expression of His-tagged enzyme in Escherichia coli, recombinant expression in transgenic Arabidopsis thaliana plants in rosette leaves
gene pip, recombinant expression in transgenic Arabidopsis thaliana plants, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene PIPA, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
in a reporter plasmids, one of the two consecutive S-layer protein (slpA) promoters (P1, P2) is placed upstream of the Lactobacillus helveticus proline iminopeptidase gene, and defined parts of the sequences upstream of the promoter are deleted. Reporter plasmid is expressed in Lactobacillus lactis and reporter gene expression is determined
PAP is overexpressed as a His-tag fusion protein under a taka-amylase gene (amyB) promoter with a limited expressing condition in Aspergillus oryzae
PAP1, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression of the enzyme in transgenic Arabidopsis thaliana plants under control of CaMV 35S promoter via the Agrobacterium tumefaciens strain GV3101 transfection system, real-time PCR enzyme expression analysis, recombinant expression as GFP-tagged enzyme in the cytoplasm
quantitative real-time PCR enzyme expression analysis, recombinant expression of the enzyme in Escherichia coli