3.4.11.24: aminopeptidase S

This is an abbreviated version!
For detailed information about aminopeptidase S, go to the full flat file.

Reaction

release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues =

Synonyms

aminolysin-S, aminopeptidase S, aminopeptidase yscCo-II, AmpS, AP, APCo-II, bacterial leucine aminopeptidase, dinuclear aminopeptidase, dizinc aminopeptidase, double-zinc aminopeptidase, leucine aminopeptidase, M28.003, S9 aminopeptidase, S9AP-St, SAP, SGAP, SGAPase, SSAP, Streptomyces aminopeptidase, Streptomyces dinuclear aminopeptidase, Streptomyces griseus aminopeptidase, Streptomyces griseus leucine aminopeptidase, transaminopeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.11 Aminopeptidases

                3.4.11.24 aminopeptidase S

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Results	in table
593	AA Sequence
3	Application
2	CAS Registry Number
11	Cloned(Commentary)
8	Crystallization (Commentary)
1	General Stability
88	Inhibitors
18	kcat/KM [mM/s]
31	Ki Value [mM]
84	KM Value [mM]
7	Localization
44	Metals/Ions
18	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
41	Organism
17	pH Optimum
8	pH Range
2	pI Value
16	Protein Variants
15	Purification (Commentary)
12	Reaction
13	Reaction Type
35	Reference
9	Source Tissue
25	Specific Activity [micromol/min/mg]
2	Storage Stability
175	Substrates and Products (Substrate)
11	Subunits
67	Synonyms
16	Temperature Optimum [°C]
5	Temperature Range [°C]
14	Temperature Stability [°C]
80	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.4.11.24 - aminopeptidase S

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant enzyme, sitting drop vapour diffusion method, the reservoir solution contains 0.1 M HEPES/NaOH, pH 7.6, 2.0 M ammonium sulfate, and 2% PEG 400, 4 weeks, X-ray diffraction structure determination and analysis at 1.8 A resolution

Staphylococcus aureus

-

665642

hanging drop vapour diffusion method, 18-25 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 3-4 weeks, X-ray diffraction structure determination at 47.2 to 1.9 A resolution and analysis

Streptomyces griseus

-

652773

protein with or without bound Zn2+ or replaced with Hg2+, hanging drop vapour diffusion method, 20 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 4-5 weeks to full size crystals, X-ray diffraction structure determination at 2.1 to 1.75 A resolution and analysis

Streptomyces griseus

-

652775

purified enzyme complexed with L-methionine, L-phenylalanine, or L-leucine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 100 mM L-methionine or 200 mM L-leucine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml of reservoir precipitant solution, 3-4 days, cyrstals of enzyme complexed with L-Phe were precipitated with 0.1 M acetate buffer, pH 5.5 instead in the same procedure within 8-10 weeks, X-ray complex structure determination at 1.6 A resolution and analysis

Streptomyces griseus

-

653836

purified enzyme complexed with methionine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 0.1 M methionine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml reservoir of the precipitant solution, 3-4 days, X-ray diffraction structure determination at 1.53 A high resolution and analysis

Streptomyces griseus

P80561

649123

purified enzyme in complex with tryptophan or 4-iodo-L-phenylalanine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM tryptophan or 2 mM 4-iodo-L-phenylalanine, equal volumes of protein and reservoir solution are mixed, the latter containing 18% w/v PEG 4000 and 0.1 M sodium acetate, pH 5.5, equilibration against 1 ml reservoir solution for 1 day, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.3 A resolution

Streptomyces griseus

-

663481

purified native enzyme in complex with product analogous weak inhibiting amino acids phenylalanine, leucine, and methionine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM L-methionine or 200 mM L-leucine, the precipitant solution contains 24% w/v PEG 4000 and 0.1 M ammonium sulfate, equilibration against 1 ml reservoir solution, microseeding, 3-4 days, with phenylalanine a protein solution containing 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM Phe is mixed with a reservoir solution containing 18% w/v PEG 4000, and 0.1 M acetate buffer, pH 5.5, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.8 A, 1.7 A, and 1.53 A resolution, respectively, structure modelling

Streptomyces griseus

P80561

649123

structure determination and analysis

Streptomyces griseus

-

665103