3.4.11.24: aminopeptidase S

This is an abbreviated version!
For detailed information about aminopeptidase S, go to the full flat file.

Reaction

release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues =

Synonyms

aminolysin-S, aminopeptidase S, aminopeptidase yscCo-II, AmpS, AP, APCo-II, bacterial leucine aminopeptidase, dinuclear aminopeptidase, dizinc aminopeptidase, double-zinc aminopeptidase, leucine aminopeptidase, M28.003, S9 aminopeptidase, S9AP-St, SAP, SGAP, SGAPase, SSAP, Streptomyces aminopeptidase, Streptomyces dinuclear aminopeptidase, Streptomyces griseus aminopeptidase, Streptomyces griseus leucine aminopeptidase, transaminopeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.11 Aminopeptidases

                3.4.11.24 aminopeptidase S

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Results	in table
593	AA Sequence
3	Application
2	CAS Registry Number
11	Cloned(Commentary)
8	Crystallization (Commentary)
1	General Stability
88	Inhibitors
18	kcat/KM [mM/s]
31	Ki Value [mM]
84	KM Value [mM]
7	Localization
44	Metals/Ions
18	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
41	Organism
17	pH Optimum
8	pH Range
2	pI Value
16	Protein Variants
15	Purification (Commentary)
12	Reaction
13	Reaction Type
35	Reference
9	Source Tissue
25	Specific Activity [micromol/min/mg]
2	Storage Stability
175	Substrates and Products (Substrate)
11	Subunits
67	Synonyms
16	Temperature Optimum [°C]
5	Temperature Range [°C]
14	Temperature Stability [°C]
80	Turnover Number [1/s]

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Cloned on EC 3.4.11.24 - aminopeptidase S

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination and analysis

Streptomyces griseus

-

665103

DNA and amino acid sequence determination, subcloning and expression of the recombinant enzyme possessing a Asp70 and Asp184 residues in Escherichia coli strain BL21(DE3), the soluble enzyme is inducible by growth on 1 M sorbitol, optimization of the expression method

Streptomyces griseus

-

664938

expression in Escherichia coli BL21

Streptomyces thermocyaneoviolaceus

-

707231, 709205

expression in Streptomyces lividans by insertional cloning and protoplast transformation, the expression system contains the constitutive Streptomyces fradiae aph promoter

Streptomyces griseus

-

653768

expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)

Staphylococcus aureus

-

665642

expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Streptomyces septatus

Q75V72

663713

gene Sgap, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli

Streptomyces griseus

-

663834

gene Sgap, screening of 21 strains with strain NBRC12875 showing the highest activity

Streptomyces griseus

Q5WA30

665735

gene SGAP, standard protoplast transformation and expression in Streptomyces lividans, excretion to the medium

Streptomyces griseus

-

653768

gene Ssap, genomic library screening, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli strain BL21(DE3)

Streptomyces septatus

Q75V72

663834