3.4.11.23: PepB aminopeptidase
This is an abbreviated version!
For detailed information about PepB aminopeptidase, go to the full flat file.
Reaction
release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln =
Synonyms
aminopeptidase, tripeptide, PepB, peptidase pepB, Salmonella enterica serovar Typhimurium peptidase B
ECTree
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Engineering
Engineering on EC 3.4.11.23 - PepB aminopeptidase
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K310V
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site-directed mutagenesis, more than 200fold reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis
R402W
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site-directed mutagenesis, reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis
additional information
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gene sequence works as functional promoter