3.4.11.19: D-stereospecific aminopeptidase

This is an abbreviated version!
For detailed information about D-stereospecific aminopeptidase, go to the full flat file.

Reaction

Release of an N-terminal D-amino acid from a peptide, Xaa-/-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-Amino acid amides and methyl esters also are hydrolysed, as is glycine amide =

Synonyms

D-aminopeptidase, DAP, GdaA, glycine D-alanine aminopeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.11 Aminopeptidases

                3.4.11.19 D-stereospecific aminopeptidase

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Results	in table
2	Activating Compound
745	AA Sequence
2	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	General Information
34	Inhibitors
3	kcat/KM [mM/s]
1	Ki Value [mM]
27	KM Value [mM]
5	Metals/Ions
10	Molecular Weight [Da]
16	Organism
1	PDB ID
6	pH Optimum
6	pH Range
3	pH Stability
3	pI Value
1	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
14	Reference
2	Specific Activity [micromol/min/mg]
64	Substrates and Products (Substrate)
7	Subunits
9	Synonyms
5	Temperature Optimum [°C]
8	Temperature Stability [°C]
10	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2550

D-Ala 4-nitroanilide

Aspergillus oryzae

G7H7Y1

pH 9.0, 30°C

731193

33 - 580

D-Ala-4-nitroanilide

3 entries

2783

Gly 4-nitroanilide

Aspergillus oryzae

G7H7Y1

pH 9.0, 30°C

731193

2100

L-Ala 4-nitroanilide

Aspergillus oryzae

G7H7Y1

pH 9.0, 30°C

731193

0.62

Nalpha, Nalpha-Ac2-L-Lys-D-Ala-D-Ala

Brucella anthropi

-

mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 37°C

670823

0.2

Nalpha-Ac-L-Lys-D-Ala-D-Ala

Brucella anthropi

-

mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 37°C

670823

0.0008 - 0.0016

nitrocefin

2 entries

33

D-Ala-4-nitroanilide

Brucella anthropi

-

mutant lacking residues 476-486 plus domains DAP-BC, pH 8.0, 30°C

670823

210

D-Ala-4-nitroanilide

Brucella anthropi

-

complex of domain DAP-A plus domains DAP-BC, pH 8.0, 30°C

670823

580

D-Ala-4-nitroanilide

Brucella anthropi

-

wild-type, pH 8.0, 30°C

670823

0.0008

nitrocefin

Brucella anthropi

-

mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 30°C

670823

0.0016

nitrocefin

Brucella anthropi

-

mutant lacking residues 476-486, pH 8.0, 30°C

670823