3.4.11.19: D-stereospecific aminopeptidase

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For detailed information about D-stereospecific aminopeptidase, go to the full flat file.

Reaction

Release of an N-terminal D-amino acid from a peptide, Xaa-/-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-Amino acid amides and methyl esters also are hydrolysed, as is glycine amide =

Synonyms

D-aminopeptidase, DAP, GdaA, glycine D-alanine aminopeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.11 Aminopeptidases

                3.4.11.19 D-stereospecific aminopeptidase

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2	Activating Compound
745	AA Sequence
2	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	General Information
34	Inhibitors
3	kcat/KM [mM/s]
1	Ki Value [mM]
27	KM Value [mM]
5	Metals/Ions
10	Molecular Weight [Da]
16	Organism
1	PDB ID
6	pH Optimum
6	pH Range
3	pH Stability
3	pI Value
1	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
14	Reference
2	Specific Activity [micromol/min/mg]
64	Substrates and Products (Substrate)
7	Subunits
9	Synonyms
5	Temperature Optimum [°C]
8	Temperature Stability [°C]
10	Turnover Number [1/s]

Engineering

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Engineering on EC 3.4.11.19 - D-stereospecific aminopeptidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

Brucella anthropi

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enzyme consists of N-terminal domain DAP-A and C-terminal domains DAP-B and DAB-C. Coexpression of DAP-A plus DAP-BC results in a complex that copurifies on Ni-NTA-column and shows a 10fold increase in KM-value compared to wild-type. Mutant lacking gamma-loop residues 476-486, no enzymic activity but small D,D-carboxypeptidase and beta-lactamase activity. Mutant lacking gamma-loop residues 476-486 plus mutation N275R, no enzymic activity but small D,D-carboxypeptidase and beta-lactamase activity.

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