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(S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide + H2O
?
-
-
-
-
?
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate + H2O
L-leucine + 4-[(E)-2-(4-aminophenyl)ethenyl-3-cyano-5,5-dimethylfuran-2(5H)-ylidene]propanedinitrile
-
-
-
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
18.7% of activity towards Leu-4-nitroanilide
-
-
?
Ala-Ala-Ala-Ala
?
-
-
-
-
?
alaninehydrazine
?
-
-
-
-
?
alanyl-7-amido-4-methylcoumarin + H2O
alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
amino acid amides + H2O
amino acid + NH3
angiotensin II + H2O
angiotensin IV + ?
-
-
through angiotensin III
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
Arg 4-nitroanilide + H2O
Arg + 4-nitroaniline
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
Arg-4-nitroanilide + H2O
L-arginine + 4-nitroaniline
-
52.5% of activity towards Leu-4-nitroanilide
-
-
?
Arg-Ala-Arg + H2O
Arg + Ala-Arg
-
Ala-p-nitroanilide is used as substrate with very low turnover rate
in the assay hydrolysis of Ala-p-nitroanilide is monitored
-
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
Arg-Gly-Asp + H2O
Arg + Gly-Asp
Arg-Gly-Gly + H2O
Arg + Gly-Gly
Arg-Gly-Leu + H2O
Arg + Gly-Leu
Arg-Gly-Phe + H2O
Arg + Gly-Phe
Arg-Gly-Ser + H2O
Arg + Gly-Ser
Arg-Ser-Arg + H2O
Arg + Ser-Arg
-
Ala-p-nitroanilide is used as substrate with very low turnover rate
in the assay hydrolysis of Ala-p-nitroanilide is monitored
-
?
Asn-Leu + H2O
Asn + Leu
-
-
?
Asp-Gln + H2O
Asp + Gln
-
-
?
Asp-His + H2O
Asp + His
-
-
?
Asp-Ile-Gly-Gly + H2O
Asp + Ile-Gly-Gly
-
-
?
Asp-Leu + H2O
Asp + Leu
-
-
?
Asp-Leu-Gly + H2O
Asp + Leu-Gly
-
-
?
Asp-Leu-Lys + H2O
Asp + Leu-Lys
-
-
?
Asp-Phe + H2O
Asp + Phe
-
-
?
Asp-Tyr + H2O
Asp + Tyr
-
-
?
benzyl-L-cysteinamide + H2O
?
bimane-S-cysteinylglycine + H2O
?
-
-
-
?
Cys-Leu + H2O
Cys + Leu
-
-
?
cysteinyl-bis-glycine + H2O
cysteine + glycine
-
-
-
?
cysteinylglycine + H2O
cysteine + glycine
DL-alpha-amino-acid amide + H2O
D-alpha-amino acid amide + L-alpha-amino acid
-
-
-
-
?
dynorphin A + H2O
?
-
-
-
?
Gln-Leu + H2O
Gln + Leu
-
-
?
Glu-Leu + H2O
Glu + Leu
-
-
?
Gly-7-amido-4-methylcoumarin + H2O
glycine + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
-
?
Gly-Val + H2O
Gly + Val
-
low activity
-
?
glycine-4-methylcoumaryl-7-amide + H2O
glycine + 7-amino-4-methylcoumarin
-
3.9% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
hArg-7-amido-4-methylcoumarin + H2O
hArg + 7-amino-4-methylcoumarin
-
-
-
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
Ile-Leu + H2O
Ile + Leu
-
-
?
kallidin + H2O
?
-
-
-
-
?
kallidin + H2O
Leu + bradykinin
-
i.e. LRPPGFSPFR
i.e. RPPGFSPFR
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
L-Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
L-Ala-7-amido-4-trifluoro-methylcoumarin + H2O
L-Ala + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
L-Ala-L-Leu + H2O
L-Ala + L-Leu
-
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarine
-
-
-
?
L-alanyl-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
L-amino acid-peptide + H2O
amino acid + peptide
L-Arg 7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
moderate activity
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
L-Arg-7-amido-4-trifluoro-methylcoumarin + H2O
L-Arg + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
L-arginine-4-nitroanilide + H2O
L-arginine + 4-nitroaniline
-
16.5% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
L-His-L-Leu + H2O
L-His + L-Leu
-
-
-
?
L-Ile-4-nitroanilide + H2O
L-Ile + 4-nitroaniline
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
L-Ile-7-amido-4-methylcoumarin + H2O
L-isoleucine + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
L-Leu beta-naphthylamide + H2O
L-Leu + beta-naphthylamine
-
-
-
?
L-Leu methyl ester
?
-
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
L-Leu-4-(phenylazo)phenylamide + H2O
L-Leu + 4-(phenylazo)phenylamine
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-Leu-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Leu
-
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
L-Leu-7-amido-4-trifluoro-methylcoumarin + H2O
L-Leu + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
L-Leu-benzyl ester + H2O
L-Leu + benzylalcohol
-
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
L-Leu-hydrazide
?
-
-
-
-
?
L-Leu-L-Arg + H2O
L-Leu + L-Arg
-
-
-
?
L-Leu-L-Asn + H2O
L-Leu + L-Asn
-
-
-
?
L-Leu-L-Asp + H2O
L-Leu + L-Asp
-
low activity
-
?
L-Leu-L-Leu + H2O
L-Leu + L-Leu
-
-
-
?
L-Leu-L-Met + H2O
L-Leu + L-Met
-
-
-
?
L-Leu-L-Phe + H2O
L-Leu + L-Phe
-
-
-
?
L-Leu-L-Ser + H2O
L-Leu + L-Ser
-
-
-
?
L-Leu-L-Tyr + H2O
L-Leu + L-Tyr
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
L-Leu-phenylazophenylamide
?
-
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-leucine 4-nitroanilide + H2O
leucine + 4-nitroaniline
L-leucine amide + H2O
L-leucine + NH3
L-leucine hydrazide + H2O
L-leucine + azide
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucine-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
-
?
L-leucine-p-nitroanilide + H2O
L-leucine + p-nitroaniline
L-leucinhydrazide + H2O
L-leucine + H2NNH2
-
-
-
-
?
L-leucyl-2-naphthylamide + H2O
L-leucine + 2-naphthylamine
L-leucyl-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucylamide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucylglycine + H2O
L-leucine + glycine
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
-
-
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
L-lysyl-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
-
-
-
?
L-lysyl-7-amido-4-methylcoumarin + H2O
L-lysine + 7-amino-4-methylcoumarin
ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin
-
-
?
L-Met 7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
moderate activity
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
L-Met-Gly + H2O
L-Met + Gly
-
-
-
?
L-Met-L-Leu + H2O
L-Met + L-Leu
-
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
L-methionyl-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
-
-
-
?
L-methionyl-7-amido-4-methylcoumarin + H2O
L-methionine + 7-amino-4-methylcoumarin
high activity
-
-
?
L-Phe 7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
L-Phe-L-Leu + H2O
L-Phe + L-Leu
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarine
-
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
L-Pro-4-nitroanilide + H2O
L-Pro + 4-nitroaniline
-
-
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
L-Pro-L-Leu + H2O
L-Pro + L-Leu
-
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
1.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarine
-
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
L-Ser-Gly + H2O
L-Ser + Gly
-
-
-
?
L-Thr 7-amido-4-methylcoumarin + H2O
L-Thr + 7-amino-4-methylcoumarin
-
low activity
-
-
?
L-Thr-4-nitroanilide + H2O
L-Thr + 4-nitroaniline
-
-
-
?
L-Thr-L-Leu + H2O
L-Thr + L-Leu
-
-
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
L-Trp-L-Leu + H2O
L-Trp + L-Leu
-
-
-
?
L-Tyr 7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
-
lower activity
-
-
?
L-Tyr-7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
6.4% activity compared to L-Leu-7-amido-4-methylcoumarin
-
-
?
L-Tyr-L-Leu + H2O
L-Tyr + L-Leu
-
-
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
8.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarine
20% activity compared to L-leucyl-4-methylcoumaryl-7-amide
-
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
L-Val-7-amido-4-methylcoumarin + H2O
L-Val + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
-
?
L-Val-L-Leu + H2O
L-Val + L-Leu
-
-
-
?
L-valine-4-methylcoumaryl-7-amide + H2O
L-valine + 7-amino-4-methylcoumarin
-
2.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-2-naphthylamide + H2O
Leu + 2-naphthylamine
-
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methyl-coumarin
-
-
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-4-trifluoroomethylcoumarin-7-amide + H2O
Leu + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
Leu-Arg-Pro-Gly + H2O
Leu + Arg-Pro-Gly
-
-
-
-
?
Leu-enkephalin
?
-
-
-
-
?
Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu + H2O
Leu + Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu
-
-
-
-
?
Leu-Gly-Gly + H2O
Leu + Gly-Gly
Leu-Gly-Tyr-Leu + H2O
Leu + Gly + Tyr + Leu
-
synthetic peptide substrate, successive release of terminal amino acids with descending activity
-
-
?
Leu-Leu-Leu + H2O
Leu + Leu-Leu
Leu-p-nitroanilide + H2O
Leu + p-nitroaniline
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
? + Ile-Asn-Phe-Glu-Lys-Leu
-
-
-
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
? + Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
-
-
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
L-leucine + Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
trimming of further model epitope precursors to antigenic peptides are also tested
-
-
?
Leu-systemin + H2O
Leu + systemin
-
LAP-A1, only in vitro
-
-
?
leucyl-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
-
-
?
leukotriene D4 + H2O
?
-
low activity
-
?
Lys 4-nitroanilide + H2O
Lys + 4-nitroaniline
Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
41.9% of activity towards Leu-4-nitroanilide
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
Met 4-nitroanilide + H2O
Met + 4-nitroaniline
Met-4-methylcoumaryl-7-amide + H2O
Met + 7-amino-4-methyl-coumarin
-
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
Met-Glu-His-Phe-Pro-Gly-Pro + H2O
?
Met-Gly + H2O
Met + Gly
-
-
?
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
-
?
N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
-
?
N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
-
?
N-(tert-butoxycarbonyl)-L-leucine + H2O
?
-
-
-
-
?
N-succinyl-L-Leu-4-nitroanilide + H2O
N-succinyl-L-Leu + 4-nitroaniline
Neurokinin A + H2O
?
-
-
-
?
Neuromedin B + H2O
?
-
-
-
?
Phe 4-nitroanilide + H2O
Phe + 4-nitroaniline
-
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methyl-coumarin
-
-
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methylcoumarin
Phg-7-amido-4-methylcoumarin + H2O
Phg + 7-amino-4-methylcoumarin
-
-
-
-
?
phosphatidylinositol-dependent kinase-1 + H2O
?
Pro-4-methylcoumaryl-7-amide + H2O
Pro + 7-amino-4-methylcoumarin
Pro-4-nitroanilide + H2O
Pro + 4-nitroaniline
-
30% of the activity with Leu-4-nitroanilide
-
-
?
S-nitrosocysteinylglycine + H2O
S-nitrosocysteine + glycine
-
-
-
?
Ser-Gly + H2O
Ser + Gly
-
-
?
Ser-Leu + H2O
Ser + Leu
-
-
?
additional information
?
-
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
-
-
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
-
-
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
42% of the activity with Leu-4-nitroanilide
-
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
-
-
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
-
-
?
Ala-Gly + H2O
Ala + Gly
-
low activity
-
?
Ala-Gly + H2O
Ala + Gly
-
-
-
?
Ala-Gly + H2O
Ala + Gly
-
low activity
-
?
Ala-Leu + H2O
Ala + Leu
-
high activity
-
?
Ala-Leu + H2O
Ala + Leu
-
-
?
Ala-Leu + H2O
Ala + Leu
-
high activity
-
?
Ala-Leu + H2O
Ala + Leu
-
high activity
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
-
-
-
?
amino acid amides + H2O
amino acid + NH3
-
-
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
-
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
slow hydrolysis: Tyr, Phe
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
D,L-norleucine
-
-
?
amino acid amides + H2O
amino acid + NH3
trout
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
Arg 4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
-
?
Arg 4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
74.5% activity compared to Leu-4-nitroanilide
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
-
?
Arg-Ala + H2O
Arg + Ala
-
-
-
?
Arg-Ala + H2O
Arg + Ala
-
-
-
?
Arg-Ala + H2O
Arg + Ala
-
-
-
?
Arg-Glu + H2O
Arg + Glu
-
low activity
-
?
Arg-Glu + H2O
Arg + Glu
-
low activity
-
?
Arg-Glu + H2O
Arg + Glu
-
low activity
-
?
Arg-Gly + H2O
Arg + Gly
-
-
-
?
Arg-Gly + H2O
Arg + Gly
-
low activity
-
?
Arg-Gly + H2O
Arg + Gly
-
-
-
?
Arg-Gly + H2O
Arg + Gly
-
low activity
-
?
Arg-Gly + H2O
Arg + Gly
-
-
-
?
Arg-Gly + H2O
Arg + Gly
-
low activity
-
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
-
low activity
-
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
-
low activity
-
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
-
low activity
-
?
Arg-Gly-Asp + H2O
Arg + Gly-Asp
-
-
-
?
Arg-Gly-Asp + H2O
Arg + Gly-Asp
-
-
-
?
Arg-Gly-Asp + H2O
Arg + Gly-Asp
-
-
-
?
Arg-Gly-Gly + H2O
Arg + Gly-Gly
-
-
-
?
Arg-Gly-Gly + H2O
Arg + Gly-Gly
-
-
-
?
Arg-Gly-Gly + H2O
Arg + Gly-Gly
-
-
-
?
Arg-Gly-Leu + H2O
Arg + Gly-Leu
-
-
-
?
Arg-Gly-Leu + H2O
Arg + Gly-Leu
-
-
-
?
Arg-Gly-Leu + H2O
Arg + Gly-Leu
-
-
-
?
Arg-Gly-Phe + H2O
Arg + Gly-Phe
-
-
-
?
Arg-Gly-Phe + H2O
Arg + Gly-Phe
-
-
-
?
Arg-Gly-Phe + H2O
Arg + Gly-Phe
-
-
-
?
Arg-Gly-Ser + H2O
Arg + Gly-Ser
-
-
-
?
Arg-Gly-Ser + H2O
Arg + Gly-Ser
-
-
-
?
Arg-Gly-Ser + H2O
Arg + Gly-Ser
-
-
-
?
Arg-Ile + H2O
Arg + Ile
-
-
-
?
Arg-Ile + H2O
Arg + Ile
-
-
-
?
Arg-Ile + H2O
Arg + Ile
-
-
-
?
Arg-Leu + H2O
Arg + Leu
-
high activity
-
?
Arg-Leu + H2O
Arg + Leu
-
high activity
-
?
Arg-Leu + H2O
Arg + Leu
-
high activity
-
?
Arg-Phe + H2O
Arg + Phe
-
-
-
?
Arg-Phe + H2O
Arg + Phe
-
-
-
?
Arg-Phe + H2O
Arg + Phe
-
-
-
?
Arg-Val + H2O
Arg + Val
-
-
-
?
Arg-Val + H2O
Arg + Val
-
-
-
?
Arg-Val + H2O
Arg + Val
-
-
-
?
benzyl-L-cysteinamide + H2O
?
-
-
-
-
?
benzyl-L-cysteinamide + H2O
?
-
-
-
-
?
Cys-Gly + H2O
Cys + Gly
-
-
?
Cys-Gly + H2O
Cys + Gly
the enzyme plays a role in glutathione turnover
-
-
?
Cys-Gly + H2O
Cys + Gly
only hydrolyzed by the Mn2+-Zn2+-enzyme, not by the Zn2+-Zn2+-enzyme
-
-
?
Cys-Gly + H2O
Cys + Gly
-
low activity
-
?
Cys-Gly + H2O
Cys + Gly
-
low activity
-
?
cysteinylglycine + H2O
cysteine + glycine
involved in turnover of glutathione
-
?
cysteinylglycine + H2O
cysteine + glycine
-
-
-
?
cysteinylglycine + H2O
cysteine + glycine
-
involved in the metabolism of glutathione and glutathione S-conjugates, major cysteinylglycine-hydrolysing activity
-
?
Gly-Leu + H2O
Gly + Leu
-
low activity
-
?
Gly-Leu + H2O
Gly + Leu
-
low activity
-
?
Gly-Leu + H2O
Gly + Leu
-
low activity
-
?
Gly-Phe + H2O
Gly + Phe
-
low activity
-
?
Gly-Phe + H2O
Gly + Phe
-
-
-
?
Gly-Phe + H2O
Gly + Phe
-
-
-
?
Gly-Phe + H2O
Gly + Phe
-
-
-
-
?
Gly-Trp + H2O
Gly + Trp
-
low activity
-
?
Gly-Trp + H2O
Gly + Trp
-
low activity
-
?
Gly-Tyr + H2O
Gly + Tyr
-
low activity
-
?
Gly-Tyr + H2O
Gly + Tyr
-
low activity
-
?
Gly-Tyr + H2O
Gly + Tyr
-
low activity
-
?
His-Gly + H2O
His + Gly
-
-
-
?
His-Gly + H2O
His + Gly
-
-
-
?
His-Gly + H2O
His + Gly
-
-
-
?
His-Leu + H2O
His + Leu
-
low activity
-
?
His-Leu + H2O
His + Leu
-
-
?
His-Leu + H2O
His + Leu
-
-
-
?
His-Leu + H2O
His + Leu
-
-
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
-
ACTH-(6-9)-PGP peptide, proteolysis of the ACTH-(6-9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. After 180 min, Arg-Trp-Pro-Gly-Pro and Trp-Pro-Gly-Pro are generated from Phe-Arg-Trp-Pro-Gly-Pro, and phenylalalnine cleavage proceeds much less efficiently than further cleavage of arginine
-
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
-
ACTH-(6-9)-PGP peptide, proteolysis of the ACTH-(6-9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. After 180 min, Arg-Trp-Pro-Gly-Pro and Trp-Pro-Gly-Pro are generated from Phe-Arg-Trp-Pro-Gly-Pro, and phenylalalnine cleavage proceeds much less efficiently than further cleavage of arginine
-
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
-
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
-
-
-
?
L-Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
-
?
L-Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
10.8% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
10.8% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
32.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
20.5% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
best substrate with L-Leu at N-terminal end
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
e.g. Leu-Leu
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
His-Lys
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
no hydrolysis of D-Leu
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Phe-Leu
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Ser-Leu
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
e.g. Leu-Leu
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Arg
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Met
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Ala-Ala-Ala-Ala
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
best substrate with L-Leu at N-terminal end
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
influence of amino acid in penultimate position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
trout
-
Leu-Gly
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
leucyl-dipeptides
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Met
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Pro-Phe-Lys
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
-
-
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
-
pre-LAP
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
34.7% activity compared to L-Leu-7-amido-4-methylcoumarin
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
62.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
about 2% activity compared to L-leucyl-4-methylcoumaryl-7-amide
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
low activity
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
-
70% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-Ile-4-nitroanilide + H2O
L-Ile + 4-nitroaniline
-
-
-
?
L-Ile-4-nitroanilide + H2O
L-Ile + 4-nitroaniline
-
-
-
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
14.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
14.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
fluorogenic substrate assay
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
-
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
pre-LAP
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
100% activity, LAP protein demonstrates preferential substrate specificity for Leu-7-amido-4-methylcoumarin
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
-
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
very slow hydrolysis
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
substituted anilides, e.g. o-, m- or p-aminobenzenesulfonic acid, o- or m-anisidine, m- or p-aminobenzenesulfonylfluoride
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
Leu substituted by Ala, Gly, Lys
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
no hydrolysis
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
very slow hydrolysis
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O
leucine + 4-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O
leucine + 4-nitroaniline
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucine hydrazide + H2O
L-leucine + azide
-
-
-
-
?
L-leucine hydrazide + H2O
L-leucine + azide
-
-
-
-
?
L-leucine hydrazide + H2O
L-leucine + azide
-
-
-
-
?
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
best synthetic substrate
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-leucine-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-leucyl-2-naphthylamide + H2O
L-leucine + 2-naphthylamine
-
-
-
-
?
L-leucyl-2-naphthylamide + H2O
L-leucine + 2-naphthylamine
-
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
moderate activity
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
ERAP1 preferentially hydrolyzes Leu-aminoacyl-aminomethylcoumarin
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
preferred substrate
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
best substrate
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-leucylglycine + H2O
L-leucine + glycine
-
-
-
-
?
L-leucylglycine + H2O
L-leucine + glycine
-
-
-
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide
-
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
-
?
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
24.5% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
24.5% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
-
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
pre-LAP
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
no activity with Z-Met-7-amido-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
no activity with Z-Met-7-amido-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
26.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
about 25% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
about 25% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
26.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
29.6% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
29.6% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
-
38.8% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
-
38.8% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
very low activity
-
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
very low activity
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
7.4% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
-
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
-
-
-
?
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
-
-
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
Leu replaced by Met, Phe
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
-
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
-
-
-
?
Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
best substrate
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
Leu replaced by Arg
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
Leu replaced by Met
-
-
?
Leu-Arg + H2O
Leu + Arg
-
-
-
?
Leu-Arg + H2O
Leu + Arg
-
-
-
?
Leu-Arg + H2O
Leu + Arg
-
-
-
?
Leu-Asn + H2O
Leu + Asn
-
-
-
?
Leu-Asn + H2O
Leu + Asn
-
-
-
?
Leu-Asn + H2O
Leu + Asn
-
-
-
?
Leu-Asp + H2O
Leu + Asp
-
low activity
-
?
Leu-Asp + H2O
Leu + Asp
-
low activity
-
?
Leu-Gly + H2O
Leu + Gly
-
-
-
-
?
Leu-Gly + H2O
Leu + Gly
-
-
?
Leu-Gly + H2O
Leu + Gly
substrate of the Mn2+-Zn2+-enzyme and the Zn2+-Zn2+-enzyme
-
-
?
Leu-Gly + H2O
Leu + Gly
-
-
-
?
Leu-Gly + H2O
Leu + Gly
-
-
-
?
Leu-Gly + H2O
Leu + Gly
-
-
-
?
Leu-Gly + H2O
Leu + Gly
-
-
-
-
?
Leu-Gly-Gly
?
-
-
-
-
?
Leu-Gly-Gly + H2O
Leu + Gly-Gly
-
-
-
?
Leu-Gly-Gly + H2O
Leu + Gly-Gly
-
-
-
?
Leu-Gly-Gly + H2O
Leu + Gly-Gly
-
-
-
?
Leu-Leu + H2O
Leu + Leu
-
-
-
?
Leu-Leu + H2O
Leu + Leu
-
-
?
Leu-Leu + H2O
Leu + Leu
-
best dipeptide substrate
-
?
Leu-Leu + H2O
Leu + Leu
-
high activity
-
?
Leu-Leu-Leu + H2O
Leu + Leu-Leu
-
-
-
-
?
Leu-Leu-Leu + H2O
Leu + Leu-Leu
-
-
-
-
?
Leu-Met + H2O
Leu + Met
-
-
-
?
Leu-Met + H2O
Leu + Met
-
-
-
?
Leu-Met + H2O
Leu + Met
-
-
-
?
Leu-p-nitroanilide + H2O
Leu + p-nitroaniline
-
-
-
-
?
Leu-p-nitroanilide + H2O
Leu + p-nitroaniline
-
-
-
-
?
Leu-Phe + H2O
Leu + Phe
-
-
-
?
Leu-Phe + H2O
Leu + Phe
-
-
-
?
Leu-Phe + H2O
Leu + Phe
-
-
-
?
Leu-Phe + H2O
Leu + Phe
-
-
-
-
?
Leu-Ser + H2O
Leu + Ser
-
-
-
?
Leu-Ser + H2O
Leu + Ser
-
-
-
?
Leu-Ser + H2O
Leu + Ser
-
-
-
?
Leu-Trp + H2O
Leu + Trp
-
-
-
?
Leu-Trp + H2O
Leu + Trp
-
-
-
?
Leu-Trp + H2O
Leu + Trp
-
-
-
?
Leu-Tyr + H2O
Leu + Tyr
-
-
-
?
Leu-Tyr + H2O
Leu + Tyr
-
-
?
Leu-Tyr + H2O
Leu + Tyr
-
-
-
?
Leu-Tyr + H2O
Leu + Tyr
-
-
-
?
Leu-Val + H2O
Leu + Val
-
-
-
?
Leu-Val + H2O
Leu + Val
-
-
-
?
Leu-Val + H2O
Leu + Val
-
-
-
-
?
Leu-Val + H2O
Leu + Val
-
-
-
?
Leu-Val + H2O
Leu + Val
-
-
-
-
?
Lys 4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
?
Lys 4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
20% activity compared to Leu-4-nitroanilide
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
?
Met 4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Met 4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
8.2% activity compared to Leu-4-nitroanilide
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Met-Glu-His-Phe-Pro-Gly-Pro + H2O
?
-
semax peptide
-
-
?
Met-Glu-His-Phe-Pro-Gly-Pro + H2O
?
-
semax peptide
-
-
?
Met-Leu + H2O
Met + Leu
-
-
-
?
Met-Leu + H2O
Met + Leu
-
-
?
Met-Leu + H2O
Met + Leu
-
high activity
-
?
Met-Leu + H2O
Met + Leu
-
high activity
-
?
N-succinyl-L-Leu-4-nitroanilide + H2O
N-succinyl-L-Leu + 4-nitroaniline
-
-
-
-
?
N-succinyl-L-Leu-4-nitroanilide + H2O
N-succinyl-L-Leu + 4-nitroaniline
-
-
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methylcoumarin
-
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methylcoumarin
-
-
-
?
Phe-Gly + H2O
Phe + Gly
-
-
-
?
Phe-Gly + H2O
Phe + Gly
-
-
-
?
Phe-Gly + H2O
Phe + Gly
-
-
-
?
Phe-Leu + H2O
Phe + Leu
-
high activity
-
?
Phe-Leu + H2O
Phe + Leu
-
-
?
Phe-Leu + H2O
Phe + Leu
-
-
-
?
Phe-Leu + H2O
Phe + Leu
-
-
-
?
phosphatidylinositol-dependent kinase-1 + H2O
?
-
i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase, which allows S6 kinase to associate with PDK1 and the enzyme upon vascular endothelial growth factor stimulation
-
-
?
phosphatidylinositol-dependent kinase-1 + H2O
?
-
i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase
-
-
?
Pro-4-methylcoumaryl-7-amide + H2O
Pro + 7-amino-4-methylcoumarin
-
-
-
?
Pro-4-methylcoumaryl-7-amide + H2O
Pro + 7-amino-4-methylcoumarin
-
-
-
?
Pro-Leu + H2O
Pro + Leu
-
-
-
?
Pro-Leu + H2O
Pro + Leu
-
-
-
?
Pro-Leu + H2O
Pro + Leu
-
-
-
?
Proteins + H2O
?
-
oxidized beta chain of insulin
-
-
?
Proteins + H2O
?
-
Leu5-enkephalin
-
-
?
Proteins + H2O
?
-
e.g. luliberin
-
-
?
Proteins + H2O
?
-
oxidized beta chain of insulin
-
-
?
Proteins + H2O
?
-
glucagon
-
-
?
Thr-Leu + H2O
Thr + Leu
-
-
-
?
Thr-Leu + H2O
Thr + Leu
-
-
?
Thr-Leu + H2O
Thr + Leu
-
-
-
?
Thr-Leu + H2O
Thr + Leu
-
-
-
?
Trp-Leu + H2O
Trp + Leu
-
low activity
-
?
Trp-Leu + H2O
Trp + Leu
-
-
?
Trp-Leu + H2O
Trp + Leu
-
-
-
?
Trp-Leu + H2O
Trp + Leu
-
low activity
-
?
Tyr-Gly + H2O
Tyr + Gly
-
-
-
?
Tyr-Gly + H2O
Tyr + Gly
-
low activity
-
?
Tyr-Gly + H2O
Tyr + Gly
-
-
-
?
Tyr-Leu + H2O
Tyr + Leu
-
-
-
?
Tyr-Leu + H2O
Tyr + Leu
-
-
?
Tyr-Leu + H2O
Tyr + Leu
-
low activity
-
?
Tyr-Leu + H2O
Tyr + Leu
-
-
-
?
Val-Gly + H2O
Val + Gly
-
-
-
?
Val-Gly + H2O
Val + Gly
-
-
-
?
Val-Gly + H2O
Val + Gly
-
-
-
?
Val-Leu + H2O
Val + Leu
-
high activity
-
?
Val-Leu + H2O
Val + Leu
-
-
?
Val-Leu + H2O
Val + Leu
-
high activity
-
?
Val-Leu + H2O
Val + Leu
-
high activity
-
?
additional information
?
-
-
not active on Ala-para-nitranilide
-
-
?
additional information
?
-
-
not active on Ala-para-nitranilide
-
-
?
additional information
?
-
rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order
-
-
?
additional information
?
-
-
rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order
-
-
?
additional information
?
-
rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
no activity is determined by using His-4-nitroanilide, Ile-4-nitroanilide, Ala-4-nitroanilide, Phe-4-nitroanilide, and Val-4-nitroanilide as substrates
-
-
?
additional information
?
-
-
no activity is determined by using His-4-nitroanilide, Ile-4-nitroanilide, Ala-4-nitroanilide, Phe-4-nitroanilide, and Val-4-nitroanilide as substrates
-
-
?
additional information
?
-
-
esterase activity
-
-
?
additional information
?
-
-
use in sequence determination
-
-
?
additional information
?
-
-
transferase activity
-
-
?
additional information
?
-
-
no hydrolysis of Xaa-Pro bond
-
-
?
additional information
?
-
molecular modelling of enzyme-substrate conformation, no activity with Asp-Gly
-
?
additional information
?
-
-
molecular modelling of enzyme-substrate conformation, no activity with Asp-Gly
-
?
additional information
?
-
-
the enzyme has tissue-specifici physiological roles, overview, active site structure and zinc-binding, mechanism
-
-
?
additional information
?
-
no activity with Ser-Gly and Asp-Gly
-
-
?
additional information
?
-
-
no activity with Ser-Gly and Asp-Gly
-
-
?
additional information
?
-
the enzyme prefers peptide substrates with bulky and hydrophobic N-terminal amino acid residues
-
-
?
additional information
?
-
-
the enzyme prefers peptide substrates with bulky and hydrophobic N-terminal amino acid residues
-
-
?
additional information
?
-
-
the enzyme acts as aminopeptidase and endopeptidase and might be a cysteine type peptidase, no activity with N-blocked substrate, Gly-4-nitroanilide, Arg-4-nitroanilide, and Lys-4-nitroanilide are poor substrates, Glu-4-nitroanilide is no substrate
-
-
?
additional information
?
-
-
enzyme activity is regulated on the transcriptional level
-
?
additional information
?
-
-
substrate specificity study, Asp-Leu, Pro-Gly, Lys-Gly, Ser-Gly, Arg-Lys, Arg-Asp, Gly-Ala, Gly-Val, Gly-Trp, Gly-Gln, Gly-Gly, Gly-Lys, Gly-His, and Leu-Pro are poor substrates, Gly-Asn, Asp-Gly, Gly-Arg, and Gly-Asn are no substrates
-
?
additional information
?
-
PepA-induced DNA deformations revealed by DNase I footprinting and AFM imaging of 1:1 stoichiometric PepA-DNA complexes
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney
-
?
additional information
?
-
-
A-LAP plays a key role in the MHC class I antigen-presentation pathway, the enzyme is involved in the renin-angiotensin-system, RAS, and angiotensin II metabolism, and plays a role in blood pressure decrease through inactivation of angiotensin II, overview
-
-
?
additional information
?
-
-
enzyme inhibition causes hypertension
-
-
?
additional information
?
-
-
the enzyme has tissue-specifici physiological roles, e.g. trimmíng of the N-terminus of antigenic peptides for presentation or in eye cataract, overview
-
-
?
additional information
?
-
-
the enzyme is involved in peptide trimming, e.g. for the generation of most HLA class I-binding peptide, in the endoplasmic reticulum in concert with other aminopeptidases, overview
-
-
?
additional information
?
-
the enzyme is involved in peptide trimming, e.g. for the generation of most HLA class I-binding peptide, in the endoplasmic reticulum in concert with other aminopeptidases, overview
-
-
?
additional information
?
-
-
the enzyme is involved in processing of precursor peptides, and in destruction of antigenic peptides to limit antigen presentation to MHC class I molecules in the endoplasmic reticulum lumen, pathway overview
-
-
?
additional information
?
-
-
the enzyme hydrolyses with Leu or Met in the P1 position
-
-
?
additional information
?
-
no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide
-
?
additional information
?
-
no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide
-
?
additional information
?
-
no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide
-
?
additional information
?
-
little or no activity against L-Val 7-amido-4-methylcoumarin and L-Pro 7-amido-4-methylcoumarin is detected
-
-
?
additional information
?
-
-
the enzyme is required for DNA synthesis and plays an important role in angiogenesis by regulating the proliferation and migration of endothelial cells, mechanism via vascular endothelial growth factor and stimulation of S6 kinase, overview
-
-
?
additional information
?
-
-
PILSAP is required for the development of vascular as well as hematopoietic system in embryoid bodies
-
-
?
additional information
?
-
-
the enzyme affects RhoA activation and that influences the proper function of endothelial cells
-
-
?
additional information
?
-
MHJ_0461 binds heparin and interacts with DNA
-
-
?
additional information
?
-
-
MHJ_0461 binds heparin and interacts with DNA
-
-
?
additional information
?
-
rMHJ_0461 binds plasminogen and facilitates plasmin conversion
-
-
?
additional information
?
-
-
rMHJ_0461 binds plasminogen and facilitates plasmin conversion
-
-
?
additional information
?
-
-
alterations in intensity and number of isozymes in cold-acclimated plants, bark and leaf
-
-
?
additional information
?
-
-
no activity towards L-prolyl-7-amido-4-methylcoumarin, L-valyl-7-amido-4-methylcoumarin, L-glycyl-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin, and tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
-
?
additional information
?
-
-
the enzyme hydrolyses with Leu or Met in the P1 position, the residues at P1', P2'a nd P3' positions influence the activity
-
-
?
additional information
?
-
-
the enzyme is important in the generation and regulation of free amino acids that are used in protein anabolism and for maintaining osmotic stability within the infected erythrocyte, enzyme inhibition, e.g. by bestatin, blocks intraerythrocytic development of malaria parasites
-
-
?
additional information
?
-
-
the enzyme functions in the terminal stages of hemoglobin digestion
-
-
?
additional information
?
-
the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs
-
-
?
additional information
?
-
-
the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs
-
-
?
additional information
?
-
-
the type of metal ion present at site 1 influences the catalytic efficiency of the enzyme for peptide substrates
-
-
?
additional information
?
-
-
the enzyme functions in the terminal stages of hemoglobin digestion
-
-
?
additional information
?
-
the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs
-
-
?
additional information
?
-
-
the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs
-
-
?
additional information
?
-
M17 leucine aminopeptidase LAP is a cytosolic metallo-exopeptidase that catalyzes the removal of amino acids from the peptide generated in the process of hemoglobin degradation
-
-
?
additional information
?
-
-
M17 leucine aminopeptidase LAP is a cytosolic metallo-exopeptidase that catalyzes the removal of amino acids from the peptide generated in the process of hemoglobin degradation
-
-
?
additional information
?
-
-
not active on Ala-para-nitranilide
-
-
?
additional information
?
-
when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
-
-
?
additional information
?
-
-
when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
-
-
?
additional information
?
-
when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity
-
-
?
additional information
?
-
-
proteolysis of ACTH-(6-9)-PGP peptide in rat blood and plasma occurs mainly under the effect of enzymes whose action is similar to leucine aminopeptidase
-
-
?
additional information
?
-
-
substrate synthesis and application to rats, overview. LC-MS analysis of reaction products after reaction of His-Phe-Arg-Trp-Pro-Gly-Pro with leucine aminopeptidase, carboxypeptidase Y, and rat nasal mucus, blood, and plasma
-
-
?
additional information
?
-
-
proteolysis of ACTH-(6-9)-PGP peptide in rat blood and plasma occurs mainly under the effect of enzymes whose action is similar to leucine aminopeptidase
-
-
?
additional information
?
-
-
substrate synthesis and application to rats, overview. LC-MS analysis of reaction products after reaction of His-Phe-Arg-Trp-Pro-Gly-Pro with leucine aminopeptidase, carboxypeptidase Y, and rat nasal mucus, blood, and plasma
-
-
?
additional information
?
-
substrate specificity study, overview, Lys-Leu, Gly-Leu, Arg-Leu, and Pro-Leu are poor or no substrates
-
?
additional information
?
-
no growth on QL, FL, YL, WL, IL, VL, KL, GL, RL, and PL
-
?
additional information
?
-
-
substrate specificity of SmLAP2, fluorogenic substrate assay, overview. No or poor activity with L-Pro 7-amido-4-methylcoumarin, L-Gly 7-amido-4-methylcoumarin, and L-Ser 7-amido-4-methylcoumarin
-
-
?
additional information
?
-
-
the enzyme probably is involved in fertility and parasite survival
-
-
?
additional information
?
-
-
the enzyme is digestive alkaline protease
-
-
?
additional information
?
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substrate specificity study, Asp-Leu, Pro-Gly, Gly-Gly, Cys-Gly, Ser-Gly, Gly-Trp, Gly-Pro, Gly-His, Gly-Lys and Lys-Gly are poor substrates, Asp-Gly, Leu-Asp, Arg-Lys, Arg-Asp, Gly-Asn, Gly-Gln, and Leu-Pro are no substrates
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?
additional information
?
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substrate specificty of wild-type and mutant enzymes, overview
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?
additional information
?
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no activity towards substrates with Asp or Glu at position P1, Arg431 and Lys354 of LAP-A1 are involved in catalysis
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?
additional information
?
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the enzyme is equally active with the synthetic substrates
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?
additional information
?
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the substrate range of LAP is quite complex. L-Leu is not the preferred substrate of the enzyme
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?
additional information
?
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use in sequence determination
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?
additional information
?
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use in sequence determination
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?
additional information
?
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no hydrolysis of Xaa-Pro bond
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?
additional information
?
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broad substrate specificity
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?
additional information
?
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substrate specificity study, Asp-Leu, Pro-Gly, Gly-Gly, Ser-Gly, Arg-Lys, Gly-Ala, Gly-Gln, Gly-His, Gly-Val and Lys-Gly are poor substrates, Asp-Gly, Arg-Asp, Gly-Arg, Gly-Asn, Gly-Lys, and Leu-Pro are no substrates
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?
additional information
?
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the enzyme has tissue-specifici physiological roles, overview
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?
additional information
?
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substrate specificity, overview, active site structure
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?
additional information
?
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substrate synthesis and assay method optimization, overview
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?
additional information
?
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2-naphthylamine can be detected with Fast Black K reactant and monitored spectrophotometrically at 590 nm
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?
additional information
?
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little or no activity against L-Val 7-amido-4-methylcoumarin and L-Pro 7-amido-4-methylcoumarin is detected
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?
additional information
?
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little or no activity against L-Val 7-amido-4-methylcoumarin and L-Pro 7-amido-4-methylcoumarin is detected
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?
additional information
?
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PepA functions as a transcriptional repressor in regulatory cascade that controls virulence gene expression in Vibrio cholerae
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?
additional information
?
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?
additional information
?
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esterase activity
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?