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0.00887
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate
-
pH 7.4, 25°C
0.0529
Ala-4-methylcoumaryl-7-amide
-
0.96 - 8.78
Ala-4-nitroanilide
3.7
Arg-4-nitroanilide
pH 8.5, 37°C
4.5
Arg-Gly
-
His-tagged recombinant enzyme, 37°C
3.3
Arg-Gly-Asp
-
His-tagged recombinant enzyme, 37°C
9.6
Arg-Gly-Phe
-
His-tagged recombinant enzyme, 37°C
1.4
Arg-Leu
-
His-tagged recombinant enzyme, 37°C
1.8
Asp-Gln
pH 8.5, 37°C, wild-type enzyme
0.6
Asp-His
pH 8.5, 37°C, wild-type enzyme
3
Asp-Ile-Gly-Gly
pH 8.5, 37°C, wild-type enzyme
0.4
Asp-Leu
pH 8.5, 37°C, wild-type enzyme
0.5
Asp-Leu-Gly
pH 8.5, 37°C, wild-type enzyme
6
Asp-Leu-Lys
pH 8.5, 37°C, wild-type enzyme
0.3
Asp-Phe
pH 8.5, 37°C, wild-type enzyme
0.3
Asp-Tyr
pH 8.5, 37°C, wild-type enzyme
0.9
Glu-Leu
pH 8.5, 37°C, wild-type enzyme
11.2
Gly-Gly
-
His-tagged recombinant enzyme, 37°C
8.6
Gly-Leu
-
His-tagged recombinant enzyme, 37°C
1
Gly-Phe
-
Zn2+-Zn2+-enzyme form
0.39
glycine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.82
hArg-7-amido-4-methylcoumarin
-
pH 9, 37°C
0.185 - 24.7
L-Ala-4-nitroanilide
0.392 - 0.442
L-Ala-7-amido-4-methylcoumarin
0.169
L-alanine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.054 - 1.16
L-alanine-4-methylcoumaryl-7-amide
0.36
L-alanyl-4-nitroanilide
-
pH 9.0, 37°C
0.79
L-Arg-7-amido-4-methylcoumarin
-
pH 9, 37°C
0.426
L-arginine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.0084 - 0.19
L-arginine-4-methylcoumaryl-7-amide
0.0414
L-arginyl-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
0.043 - 0.062
L-Cys-7-amido-4-methylcoumarin
0.261
L-Ile-4-nitroanilide
pH 8, 20°C
0.054 - 0.077
L-Ile-7-amido-4-methylcoumarin
0.04456
L-Leu 7-amido-4-methylcoumarin
pH 7.2, 28°C, recombinant enzyme
8.5
L-Leu methyl ester
-
-
0.25
L-Leu-2-naphthylamide
0.035 - 12.7
L-Leu-4-nitroanilide
0.012 - 1.38
L-Leu-7-amido-4-methylcoumarin
1.56
L-Leu-benzyl ester
-
-
17.5 - 342
L-Leu-hydrazide
0.14
L-Leu-phenylazophenylamide
-
-
0.107
L-leucine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.015 - 0.35
L-leucine-4-methylcoumaryl-7-amide
0.14 - 3
L-leucine-4-nitroanilide
0.5
L-leucine-p-naphthylamide
-
-
0.084
L-leucyl-2-naphthylamide
-
pH 7.0, temperature not specified in the publication
0.00925
L-leucyl-4-nitroanilide
-
pH 9.0, 37°C
0.00155 - 0.0135
L-leucyl-7-amido-4-methylcoumarin
14.2
L-leucylamide
-
native enzyme, pH 8.0, 25°C
0.094 - 8.9
L-Lys-4-nitroanilide
0.3641 - 0.81
L-lysine-4-methylcoumaryl-7-amide
0.099
L-Lysyl-4-nitroanilide
-
pH 9.0, 37°C
0.053 - 0.0597
L-Met-4-nitroanilide
0.105 - 0.154
L-Met-7-amido-4-methylcoumarin
0.12
L-methionine-4-methyl-coumaryl-7-amide
-
0.1092
L-methionine-4-methylcoumaryl-7-amide
pH 8.5, 37°C
0.083
L-methionyl-4-nitroanilide
-
pH 9.0, 37°C
0.0038
L-methionyl-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
0.0842 - 0.091
L-Phe-4-nitroanilide
0.3
L-phenylalanine-4-methyl-coumaryl-7-amide
-
0.01
L-phenylalanine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.108 - 0.245
L-phenylalanine-4-methylcoumaryl-7-amide
0.782
L-phenylalanine-4-nitroanilide
-
pH 8.5, 50°C
0.216 - 13.1
L-Pro-4-nitroanilide
0.124
L-proline-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.025 - 0.05
L-proline-4-methylcoumaryl-7-amide
5.2
L-Ser-Gly
pH 7.4, 25°C
0.198 - 0.204
L-Thr-4-nitroanilide
0.072 - 0.118
L-Trp-7-amido-4-methylcoumarin
0.055
L-tyrosine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.372
L-Val-4-nitroanilide
pH 8, 20°C
0.019
L-valine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.01212
Leu-4-methylcoumaryl-7-amide
-
0.22 - 8.8
Leu-4-nitroanilide
35.8
Leu-7-amino-4-trilfluoromethylcoumarin
-
-
0.17 - 0.97
Leu-p-nitroanilide
0.06
Leu-Phe
-
Zn2+-Zn2+-enzyme form
1
Leu-Tyr
pH 8.5, 37°C, wild-type enzyme
0.01 - 1.25
leucine-7-amido-4-methylcoumarin
0.59 - 3.6
Lys-4-nitroanilide
0.17 - 4.4
Met-4-nitroanilide
0.065
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide
-
pH not specified in the publication, temperature not specified in the publication
0.065
N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide
-
pH 8.0, 31°C
0.00792
Phe-4-methylcoumaryl-7-amide
-
1.33
Phg-7-amido-4-methylcoumarin
-
pH 9, 37°C
0.2349
Pro-4-methylcoumaryl-7-amide
-
0.194
Pro-4-nitroanilide
-
pH 7.0, 37°C
additional information
additional information
-
0.96
Ala-4-nitroanilide
-
pH 7.5, 30°C
8.78
Ala-4-nitroanilide
-
pH 7.0, 37°C
0.42
Cys-Gly
pH 6.9, 25°C
0.57
Cys-Gly
pH 7.4, 25°C
0.59
Cys-Gly
pH 8.3, 25°C
0.185
L-Ala-4-nitroanilide
pH 8, 20°C
0.202
L-Ala-4-nitroanilide
pH 8, 20°C
24.7
L-Ala-4-nitroanilide
pH 7.5, 80°C
0.392
L-Ala-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.408
L-Ala-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.442
L-Ala-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.054
L-alanine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.095
L-alanine-4-methylcoumaryl-7-amide
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.2396
L-alanine-4-methylcoumaryl-7-amide
pH 8.5, 37°C
1.16
L-alanine-4-methylcoumaryl-7-amide
-
0.0084
L-arginine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.189
L-arginine-4-methylcoumaryl-7-amide
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.19
L-arginine-4-methylcoumaryl-7-amide
-
0.043
L-Cys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.044
L-Cys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.062
L-Cys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.054
L-Ile-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.06
L-Ile-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.077
L-Ile-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.25
L-Leu-2-naphthylamide
-
-
0.25
L-Leu-2-naphthylamide
-
similar value
0.035
L-Leu-4-nitroanilide
pH 8, 20°C
0.036
L-Leu-4-nitroanilide
pH 8, 20°C
0.5
L-Leu-4-nitroanilide
-
-
1.1
L-Leu-4-nitroanilide
-
-
1.2
L-Leu-4-nitroanilide
pH 7.5, 50°C, enzyme activated by heating at 70°C
1.8
L-Leu-4-nitroanilide
pH 7.5, 50°C
6
L-Leu-4-nitroanilide
-
Zn2+-Zn2+-enzyme form
6.3
L-Leu-4-nitroanilide
pH 7.5, 35°C
12.7
L-Leu-4-nitroanilide
pH 7.5, 80°C
0.012
L-Leu-7-amido-4-methylcoumarin
native enzyme, pH 7.5, 37°C
0.0182
L-Leu-7-amido-4-methylcoumarin
pH 7.5, 37°, Vmax: 10.7 microM/min
0.167
L-Leu-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.1859
L-Leu-7-amido-4-methylcoumarin
recombinant enzyme, pH 7.5, 37°C
0.189
L-Leu-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.229
L-Leu-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
1.38
L-Leu-7-amido-4-methylcoumarin
-
pH 9, 37°C
1.8
L-Leu-Gly
-
wild-type, pH 9.2, 37°C
2
L-Leu-Gly
-
mutant E429D, pH 9.2, 37°C
5.1
L-Leu-Gly
-
mutant R431K, pH 9.2, 37°C
7.2
L-Leu-Gly
-
similar value
7.2
L-Leu-Gly
-
similar value
17.5
L-Leu-hydrazide
-
-
0.51
L-Leu-L-Val
-
-
0.51
L-Leu-L-Val
-
similar value
0.015
L-leucine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.102
L-leucine-4-methylcoumaryl-7-amide
pH 8.5, 37°C
0.102
L-leucine-4-methylcoumaryl-7-amide
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.109
L-leucine-4-methylcoumaryl-7-amide
pH 8.0, 37°C, native enzyme
0.19
L-leucine-4-methylcoumaryl-7-amide
-
0.35
L-leucine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, recombinant enzyme in presence of Zn2+ and Mg2+, best ion combination at metal binding sites 1 and 2
0.14
L-leucine-4-nitroanilide
-
-
0.244
L-leucine-4-nitroanilide
-
pH 8.5, 50°C
3
L-leucine-4-nitroanilide
-
pH 8.0, 31°C
3
L-leucine-4-nitroanilide
-
pH not specified in the publication, temperature not specified in the publication
0.00155
L-leucyl-7-amido-4-methylcoumarin
-
in 25 mM sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 0.02% NaN3, at 37°C
0.0135
L-leucyl-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
0.094
L-Lys-4-nitroanilide
pH 8, 20°C
0.103
L-Lys-4-nitroanilide
pH 8, 20°C
5
L-Lys-4-nitroanilide
pH 7.5, 35°C
8.9
L-Lys-4-nitroanilide
pH 7.5, 80°C
0.3641
L-lysine-4-methylcoumaryl-7-amide
pH 8.5, 37°C
0.81
L-lysine-4-methylcoumaryl-7-amide
-
0.053
L-Met-4-nitroanilide
pH 8, 20°C
0.0597
L-Met-4-nitroanilide
pH 8, 20°C
0.105
L-Met-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.137
L-Met-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.154
L-Met-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
1.3
L-Met-Gly
pH 8.3, 25°C
2.8
L-Met-Gly
pH 6.9, 25°C
0.0842
L-Phe-4-nitroanilide
pH 8, 20°C
0.091
L-Phe-4-nitroanilide
pH 8, 20°C
0.108
L-phenylalanine-4-methylcoumaryl-7-amide
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.245
L-phenylalanine-4-methylcoumaryl-7-amide
pH 8.5, 37°C
0.216
L-Pro-4-nitroanilide
pH 8, 20°C
0.239
L-Pro-4-nitroanilide
pH 8, 20°C
13.1
L-Pro-4-nitroanilide
pH 7.5, 80°C
0.025
L-proline-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.05
L-proline-4-methylcoumaryl-7-amide
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.198
L-Thr-4-nitroanilide
pH 8, 20°C
0.204
L-Thr-4-nitroanilide
pH 8, 20°C
0.072
L-Trp-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.104
L-Trp-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.118
L-Trp-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant His-tagged enzyme
0.22
Leu-4-nitroanilide
-
pH 7.5, 30°C
0.248
Leu-4-nitroanilide
-
pH 7.0, 37°C
0.97
Leu-4-nitroanilide
pH 8.5, 50°C, recombinant enzyme
1.28
Leu-4-nitroanilide
-
-
2
Leu-4-nitroanilide
-
Mg2+-Zn2+-enzyme form
4.1
Leu-4-nitroanilide
-
Zn2+-Zn2+-enzyme form
4.5
Leu-4-nitroanilide
pH 8.5, 37°C
8.8
Leu-4-nitroanilide
-
pH 8.0, 37°C
1.5
Leu-Gly
-
37°C
2.2
Leu-Gly
-
free recombinant enzyme, 37°C
3.2
Leu-Gly
-
His-tagged recombinant enzyme, 37°C
7.2
Leu-Gly
-
similar value
8.4
Leu-Gly
-
Mg2+-Zn2+-enzyme form
1.9
Leu-Gly-Gly
-
His-tagged recombinant enzyme, 37°C
2.9
Leu-Leu
-
His-tagged recombinant enzyme, 37°C
4
Leu-Leu
pH 8.5, 37°C, wild-type enzyme
0.17
Leu-p-nitroanilide
-
pH 7.0, 37°C
0.22
Leu-p-nitroanilide
-
pH 7.0, 37°C
0.23
Leu-p-nitroanilide
wild type enzyme, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2
0.52
Leu-p-nitroanilide
mutant enzyme N345Q, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2
0.97
Leu-p-nitroanilide
mutant enzyme N345L, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2
4.02
leucinamide
-
-
4.02
leucinamide
-
similar value
4.02
leucinamide
-
similar value
17
leucinamide
-
similar value
0.01
leucine-7-amido-4-methylcoumarin
-
-
1.25
leucine-7-amido-4-methylcoumarin
-
-
0.59
Lys-4-nitroanilide
-
pH 7.5, 30°C
3.6
Lys-4-nitroanilide
pH 8.5, 37°C
0.17
Met-4-nitroanilide
-
pH 7.5, 30°C
4.4
Met-4-nitroanilide
pH 8.5, 37°C
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
metal ion binding kinetics to the recombinant enzyme, metal ion occupying site 1 can influence enzyme substrate kinetics, overview
-
additional information
additional information
-
dynamics of ACTH-(6-9)-PGP peptide hydrolysis with leucine aminopeptidase and carboxypeptidase Y and reaction kinetics, overview
-