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3.4.11.1: leucyl aminopeptidase

This is an abbreviated version!
For detailed information about leucyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.1

Reaction

release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low =

Synonyms

A-LAP, acidic M17 leucine aminopeptidase, AcLAP, adipocyte-derived leucine aminopeptidase, Aminopeptidase, aminopeptidase A, Aminopeptidase A/I, aminopeptidase I, aminopeptidase II, Aminopeptidase III, aminopeptidase LAP2, aminopeptidase N, AP 28, AP 56, APDkam589, APN, b/LAP, bovine lens/leucine aminopeptidase, BSAP, cathepsin III, CsLAP1, CsLAP2, cysteinyl-glycine hydrolysing activity, cysteinylglycine-hydrolysing activity, cytosol aminopeptidase, DR57, EC 3.4.1.1, Eg-LAP, endoplasmic reticulum aminopeptidase, ER-aminopeptidase-1, ERAP, ERAP1, ERAP2, FgLAP, FrvX, FTBL protein, FTBL proteins, HSA, L-leucine aminopeptidase, la, LAP, LAP yspII, LAP-A, LAP-N, LAP1, LAP2, lap3, LapA, LAPc, LeuAP, leucinamide aminopeptidase, leucinaminopeptidase, leucine amino peptidase, leucine aminopeptidase, leucine aminopeptidase 1, leucine aminopeptidase 2, leucine aminopeptidase 3, leucine aminopeptidase A, leucine aminopeptidase LAP-N, leucine aminopeptidase N, leucine aminopeptidase,, leucine aminopeptidase-A, Leucyl aminopeptidase, leucyl aminopeptidase (animal), leucyl aminopeptidase (plant), leucyl aminopeptidase yspII, leucyl peptidase, leucylaminopeptidase, leucylpeptidase, LmLAP-A, M17 family leucyl aminopeptidase metalloprotease, M17 LAP, M17 leucine amino peptidase, M17 leucine aminopeptidase, M17 leucyl aminopeptidase, M42 aminopeptidase, major leucyl aminopeptidase, More, PA2939, PaAP, PepA, PepA peptidase A, PepB, peptidase B, peptidase II, peptidase S, pepZ, PfLAP, PH1527, PhTET2, PILS-AP, PILSAP, Placental leucine aminopeptidase, PLAP, proline aminopeptidase, Prolyl aminopeptidase, proteins, specific or class, FTBL, PtLAP, puromycin insensitive leucyl-specific aminopeptidase, puromycin-insensitive leucine specific aminopeptidase, puromycin-insensitive leucyl-specific aminopeptidase, PVX_118180, rLAP, rMHJ_0461, S-Lap1, S-Lap2, S-Lap3, S-Lap4, S-Lap5, S-Lap6, S-Lap7, S-Lap8, SmLAP1, SmLAP2, Smp_03000, Smp_083870, sperm-leucylaminopeptidase, TbLAP-A, TbLAP1, TcLAP-A, TM0042, TpLAP, XoLAP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.1 leucyl aminopeptidase

KM Value

KM Value on EC 3.4.11.1 - leucyl aminopeptidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00887
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate
-
pH 7.4, 25°C
0.0529
Ala-4-methylcoumaryl-7-amide
-
0.96 - 8.78
Ala-4-nitroanilide
3.3
Ala-Ala-Ala-Ala
-
-
6.9
alaninehydrazide
-
-
3.7
Arg-4-nitroanilide
pH 8.5, 37°C
4.5
Arg-Gly
-
His-tagged recombinant enzyme, 37°C
3.3
Arg-Gly-Asp
-
His-tagged recombinant enzyme, 37°C
9.6
Arg-Gly-Phe
-
His-tagged recombinant enzyme, 37°C
1.4
Arg-Leu
-
His-tagged recombinant enzyme, 37°C
1.8
Asp-Gln
pH 8.5, 37°C, wild-type enzyme
0.6
Asp-His
pH 8.5, 37°C, wild-type enzyme
3
Asp-Ile-Gly-Gly
pH 8.5, 37°C, wild-type enzyme
0.4
Asp-Leu
pH 8.5, 37°C, wild-type enzyme
0.5
Asp-Leu-Gly
pH 8.5, 37°C, wild-type enzyme
6
Asp-Leu-Lys
pH 8.5, 37°C, wild-type enzyme
0.3
Asp-Phe
pH 8.5, 37°C, wild-type enzyme
0.3
Asp-Tyr
pH 8.5, 37°C, wild-type enzyme
0.42 - 0.59
Cys-Gly
0.9
Glu-Leu
pH 8.5, 37°C, wild-type enzyme
11.2
Gly-Gly
-
His-tagged recombinant enzyme, 37°C
8.6
Gly-Leu
-
His-tagged recombinant enzyme, 37°C
1
Gly-Phe
-
Zn2+-Zn2+-enzyme form
0.39
glycine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.82
hArg-7-amido-4-methylcoumarin
-
pH 9, 37°C
0.185 - 24.7
L-Ala-4-nitroanilide
0.392 - 0.442
L-Ala-7-amido-4-methylcoumarin
0.169
L-alanine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.054 - 1.16
L-alanine-4-methylcoumaryl-7-amide
0.36
L-alanyl-4-nitroanilide
-
pH 9.0, 37°C
0.79
L-Arg-7-amido-4-methylcoumarin
-
pH 9, 37°C
0.426
L-arginine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.0084 - 0.19
L-arginine-4-methylcoumaryl-7-amide
0.0414
L-arginyl-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
0.043 - 0.062
L-Cys-7-amido-4-methylcoumarin
0.261
L-Ile-4-nitroanilide
pH 8, 20°C
0.054 - 0.077
L-Ile-7-amido-4-methylcoumarin
0.04456
L-Leu 7-amido-4-methylcoumarin
pH 7.2, 28°C, recombinant enzyme
8.5
L-Leu methyl ester
-
-
0.25
L-Leu-2-naphthylamide
0.035 - 12.7
L-Leu-4-nitroanilide
0.012 - 1.38
L-Leu-7-amido-4-methylcoumarin
1.56
L-Leu-benzyl ester
-
-
1.8 - 7.2
L-Leu-Gly
17.5 - 342
L-Leu-hydrazide
0.51
L-Leu-L-Val
0.14
L-Leu-phenylazophenylamide
-
-
0.107
L-leucine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.015 - 0.35
L-leucine-4-methylcoumaryl-7-amide
0.14 - 3
L-leucine-4-nitroanilide
0.5
L-leucine-p-naphthylamide
-
-
0.084
L-leucyl-2-naphthylamide
-
pH 7.0, temperature not specified in the publication
0.00925
L-leucyl-4-nitroanilide
-
pH 9.0, 37°C
0.00155 - 0.0135
L-leucyl-7-amido-4-methylcoumarin
14.2
L-leucylamide
-
native enzyme, pH 8.0, 25°C
0.094 - 8.9
L-Lys-4-nitroanilide
0.3641 - 0.81
L-lysine-4-methylcoumaryl-7-amide
0.099
L-Lysyl-4-nitroanilide
-
pH 9.0, 37°C
0.053 - 0.0597
L-Met-4-nitroanilide
0.105 - 0.154
L-Met-7-amido-4-methylcoumarin
1.3 - 2.8
L-Met-Gly
0.12
L-methionine-4-methyl-coumaryl-7-amide
-
0.1092
L-methionine-4-methylcoumaryl-7-amide
pH 8.5, 37°C
0.083
L-methionyl-4-nitroanilide
-
pH 9.0, 37°C
0.0038
L-methionyl-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
0.0842 - 0.091
L-Phe-4-nitroanilide
0.3
L-phenylalanine-4-methyl-coumaryl-7-amide
-
0.01
L-phenylalanine-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.108 - 0.245
L-phenylalanine-4-methylcoumaryl-7-amide
0.782
L-phenylalanine-4-nitroanilide
-
pH 8.5, 50°C
0.216 - 13.1
L-Pro-4-nitroanilide
0.124
L-proline-4-methylcoumarin-7-amide
-
pH 8.0, 37°C, recombinant GST-tagged enzyme
0.025 - 0.05
L-proline-4-methylcoumaryl-7-amide
5.2
L-Ser-Gly
pH 7.4, 25°C
0.198 - 0.204
L-Thr-4-nitroanilide
0.072 - 0.118
L-Trp-7-amido-4-methylcoumarin
0.055
L-tyrosine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.372
L-Val-4-nitroanilide
pH 8, 20°C
0.019
L-valine-4-methylcoumaryl-7-amide
-
pH 7.0, 37°C
0.01212
Leu-4-methylcoumaryl-7-amide
-
0.22 - 8.8
Leu-4-nitroanilide
35.8
Leu-7-amino-4-trilfluoromethylcoumarin
-
-
1.5 - 8.4
Leu-Gly
1.9 - 3.7
Leu-Gly-Gly
2.9 - 4
Leu-Leu
0.17 - 0.97
Leu-p-nitroanilide
0.06
Leu-Phe
-
Zn2+-Zn2+-enzyme form
1
Leu-Tyr
pH 8.5, 37°C, wild-type enzyme
4.02 - 17
leucinamide
0.01 - 1.25
leucine-7-amido-4-methylcoumarin
0.59 - 3.6
Lys-4-nitroanilide
0.17 - 4.4
Met-4-nitroanilide
1.5
Met-Gly
pH 7.4, 25°C
0.065
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide
-
pH not specified in the publication, temperature not specified in the publication
0.065
N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide
-
pH 8.0, 31°C
0.00792
Phe-4-methylcoumaryl-7-amide
-
1.33
Phg-7-amido-4-methylcoumarin
-
pH 9, 37°C
0.2349
Pro-4-methylcoumaryl-7-amide
-
0.194
Pro-4-nitroanilide
-
pH 7.0, 37°C
5
Ser-Gly
pH 8.3, 25°C
additional information
additional information
-