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3.4.11.1: leucyl aminopeptidase

This is an abbreviated version!
For detailed information about leucyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.1

Reaction

release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low =

Synonyms

A-LAP, acidic M17 leucine aminopeptidase, AcLAP, adipocyte-derived leucine aminopeptidase, Aminopeptidase, aminopeptidase A, Aminopeptidase A/I, aminopeptidase I, aminopeptidase II, Aminopeptidase III, aminopeptidase LAP2, aminopeptidase N, AP 28, AP 56, APDkam589, APN, b/LAP, bovine lens/leucine aminopeptidase, BSAP, cathepsin III, CsLAP1, CsLAP2, cysteinyl-glycine hydrolysing activity, cysteinylglycine-hydrolysing activity, cytosol aminopeptidase, DR57, EC 3.4.1.1, Eg-LAP, endoplasmic reticulum aminopeptidase, ER-aminopeptidase-1, ERAP, ERAP1, ERAP2, FgLAP, FrvX, FTBL protein, FTBL proteins, HSA, L-leucine aminopeptidase, la, LAP, LAP yspII, LAP-A, LAP-N, LAP1, LAP2, lap3, LapA, LAPc, LeuAP, leucinamide aminopeptidase, leucinaminopeptidase, leucine amino peptidase, leucine aminopeptidase, leucine aminopeptidase 1, leucine aminopeptidase 2, leucine aminopeptidase 3, leucine aminopeptidase A, leucine aminopeptidase LAP-N, leucine aminopeptidase N, leucine aminopeptidase,, leucine aminopeptidase-A, Leucyl aminopeptidase, leucyl aminopeptidase (animal), leucyl aminopeptidase (plant), leucyl aminopeptidase yspII, leucyl peptidase, leucylaminopeptidase, leucylpeptidase, LmLAP-A, M17 family leucyl aminopeptidase metalloprotease, M17 LAP, M17 leucine amino peptidase, M17 leucine aminopeptidase, M17 leucyl aminopeptidase, M42 aminopeptidase, major leucyl aminopeptidase, More, PA2939, PaAP, PepA, PepA peptidase A, PepB, peptidase B, peptidase II, peptidase S, pepZ, PfLAP, PH1527, PhTET2, PILS-AP, PILSAP, Placental leucine aminopeptidase, PLAP, proline aminopeptidase, Prolyl aminopeptidase, proteins, specific or class, FTBL, PtLAP, puromycin insensitive leucyl-specific aminopeptidase, puromycin-insensitive leucine specific aminopeptidase, puromycin-insensitive leucyl-specific aminopeptidase, PVX_118180, rLAP, rMHJ_0461, S-Lap1, S-Lap2, S-Lap3, S-Lap4, S-Lap5, S-Lap6, S-Lap7, S-Lap8, SmLAP1, SmLAP2, Smp_03000, Smp_083870, sperm-leucylaminopeptidase, TbLAP-A, TbLAP1, TcLAP-A, TM0042, TpLAP, XoLAP

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.1 leucyl aminopeptidase

Cloned

Cloned on EC 3.4.11.1 - leucyl aminopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a lentivirus is used to induce K-562 cells to overexpress LAP3 (K562-LAP3)
a myc-His-tagged soluble version starting at amino acid position 155 (lacking the putative transmembrane region) expressed in 293F cells
-
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein
DNA and amino acid sequence determination and analysis, expression of His-tagged LAP in Escherichia coli
-
DNA and amino acid sequence determination and analysis, overexpression of the full-length gene in parasite cytosol, transgenic parasites are more resistant to bestatin
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of His6-tagged enzyme in Spodoptera frugiperda Sf9 insect cells using the baculovirus transfection system
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DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of GST-tagged LAP in Escherichia coli DH12S cells
DNA sequence determination, overexpression of a LAP fusion protein in Escherichia coli
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DNA sequence determination, overexpression of mature LAP-A, His6-tagged LAP-A and pre-LAP-A in Escherichia coli
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DNA sequence determination, overexpression of pre-LAP protein in Escherichia coli
-
expressed as a His-tagged fusion protein in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli M15 cells
expressed in Sf9 cells using the baculovirus system
expression in COS-7 and in CHO cells, secretion from recombinant COS-7 cells
-
expression in Escherichia coli
expression in Sf9 cells
expression of a DNA library in Salmonella typhimurium, DNA and sequence determination and analysis, expression of the full length enzyme in Salmonella typhimurium strain TN5179
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), transient expression in COS-7 cells and in HEK-293 cells
-
expression of LAP in Escherichia coli strain BL21
from an adipocyte tissue cDNA library
-
gene LAP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene LAP, quantitative real-time PCR expression analysis, recombinant expression of V5-tagged TbLAP1 in Trypanosoma brucei, recombinant ectopic expression of HA-tagged TbLAP1 in procyclic Trypanosoma brucei
gene lap, recombinant expression of His-tagged enzyme in Escherichia coli
gene lap, recombinant expression of His-tagged enzyme in Escherichia coli, overexpression of c-Myc-tagged LAP-A in Trypanosoma brucei parasites cytosol
gene LAP, sequence comparions and phylogenetic analysis, recombinant expression of His6-tagged TF-fused AcLAPr in Escherichia coli strain BL21, recombinant expression of EGFP-tagged enzyme in Acanthamoeba castellanii trophozoites
gene lap, sequence comparisons, functional recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Transetta (DE3)
gene lap1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene lap1, recombinant overexpression of truncated Lap1 without a signal peptide in Pichia pastoris
gene lap2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene paaP, cloning in Escherichia coli strain DH5alpha
gene pepA, recombinant overexpression in Escherichia coli strain JM101
genotyping
-
His-tagged LAP expression in insect cells
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli M15 [pREP4]
His-tagged protein expressed in Escherichia coli Rosetta-gami B (DE3) pLysS
-
His-tagged thioredoxin-fusion protein expressed in Escherichia coli
-
isozyme ERAP1, DNA and amino acid sequence determination and analysis, expression analysis in EBV-B and tumor cell lines, recombinant expression in HeLa cells
isozyme ERAP2, DNA and amino acid sequence determination and analysis, expression analysis in EBV-B and tumor cell lines, recombinant expression in HeLa cells
overexpressed in Aspergillus oryzae RIB40
-
overexpression in Escherichia coli
overexpression of free and His-tagged enzyme in peptidase A-deficient Escherichia coli strain DS957 xerB:Tn5
-
overexpression of His-tagged wild-type and mutants in Escherichia coli
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phylogenetic tree, expression of GST-tagged LAP in Escherichia coli strain BL21, construction of a HA-tagged enzyme
-
recombinant CsLAP2 is expressed and purified in Escherichia coli BL21
recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted, the recombinant enzyme level is 7.1 times that of wild-type Bacillus subtilis strain Zj016, subcloning in Escherichia coli strain JM109
SmLAP1 and SmLAP2, cloning of two discrete LAPs genes, DNA and amino acid sequence determination, genome loci structure, phylogenetic tree, overview
TpLAP, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of soluble GST-tagged TpLAP in Escherichia coli strain Transetta (DE3), subcloning in Escherichia coli strain DH5alpha cells