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3.3.2.9: microsomal epoxide hydrolase

This is an abbreviated version!
For detailed information about microsomal epoxide hydrolase, go to the full flat file.

Word Map on EC 3.3.2.9

Reaction

1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine
+
H2O
=
2-({[(4-methoxyphenyl)methyl](methyl)amino}methyl)-2-methylpropane-1,3-diol

Synonyms

alpha,beta fold epoxide hydrolase, alpha,beta-fold type EH, E1-b', EC 3.3.2.3, EC 4.2.1.63, EC 4.2.1.64, EH1, EH2, Eha, EHb, EPHX, EPHX 1, EPHX1, EPOX, epoxide hydrolase, HYL1, JH epoxide hydrolase, JHEH, juvenile hormone epoxide hydrolase, mEH, mEH-like protein, mEH1, mEPHX, microsomal, microsomal EH, microsomal EPHX1, Microsomal epoxide hydrolase, microsomal epoxide hydrolase 1, microsomal epoxide hydrolase-like protein, microsomal xenobiotic epoxide hydrolase, More, PNSO hydrolase, SEH, styrene-epoxide hydrolase, Tcjheh-r1, Tcjheh-r3, XEHase, xenobiotic epoxide hydrolase

ECTree

     3 Hydrolases
         3.3 Acting on ether bonds
             3.3.2 Ether hydrolases
                3.3.2.9 microsomal epoxide hydrolase

Cloned

Cloned on EC 3.3.2.9 - microsomal epoxide hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus expression system
expressed in a baculovirus expression system in High Five insect cell cultures (derived from Trichoplusia ni)
-
expressed in Escherichia coli
expressed in HEK-293A cells, HEK-293T cells, and MCF-7 cells
-
expressed in Sf-9 insect cells
expressed in Sf9 insect cells
expression analysis
-
expression in baculoviral system
expression in Escherichia coli
expression of His-tagged enzyme in Saccharomyces cerevisiae strain WA6, the N-terminal His-tag reduces enzyme activity by 75%, therefore a C-terminal His-tag is used
-
expression pattern of mEH
-
expression using a baculovirus expression system
functional expression in Escherichia coli
-
gene EPHX1 is located on chromosome 1, DNA and amino acid sequence determination and analysis, expression of liver enzyme in Schizosaccharomyces pombe, expression in Cos-1 cells, expression using the baculovirus system
-
gene Ephx1 or meh, quantitative RT-PCR expression analysis
gene EPHX1, located on chromosome 1
-
gene EPHX1, located on chromosome 1 (1q42.12) consists of nine exons, and spans about 35 kb, exons 2-9 of EPHX1 encode three transcription variants differing in the 5'-untranslated region, while each translated protein product has 455 amino acids. Human EPHX1 expression in the liver is selectively driven by the proximal E1 promoter, but an alternative promoter region (E1-b promoter) drives expression in other tissues from both adult and fetal sources, e.g. an EPHX1 transcript (E1-b') generated from the upstream promoter is reported to be expressed in a tissue-selective manner with the highest level in human ovary. The E1-b promoter region harbors several functionally important polymorphisms including a double Alu insertion, which may influence interindividual susceptibility to toxicity of xenobiotics
gene EPHX1, mEH genotyping
gene EPHX1, sequencing of the EPHX1 5' region (-784/-1), 9 exons, intron 1, exon intron boundaries and the 3' untranslated region, analysis of EPHX1 transcriptional activity mediated by the proximal promoter region in HepG2 cells
genotyping
-
genotyping analysis of several humans of different origin
-
genotyping of 3553 humans
-
genotyping of 497 humans
-
genotyping of patients from the Copenhagen City Heart Study for EPHX1 polymorphisms
-
screening of different Karenia brevis transcriptome libraries and enzyme cloning, recombinant expression of His-tagged mEH isozymes in Escherichia coli
-
the E1-b promoter functions as the primary driver of EPHX1 expression in human tissues, E1 promoter directs expression only in the liver, analysis of promoters and regulation enzyme expression in tissues, expression in Hep-G2 cells and 293A cells, co-expression of GATA-4, overview