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3.3.2.8: limonene-1,2-epoxide hydrolase

This is an abbreviated version!
For detailed information about limonene-1,2-epoxide hydrolase, go to the full flat file.

Word Map on EC 3.3.2.8

Reaction

1,2-epoxymenth-8-ene
+
H2O
=
menth-8-ene-1,2-diol

Synonyms

CH55-LEH, LEH, limA, limonene 1,2-epoxide hydrolase, limonene epoxide hydrolase, limonene oxide hydrolase, limonene-1,2-epoxide hydrolase, Re-LEH, Tomsk-LEH

ECTree

     3 Hydrolases
         3.3 Acting on ether bonds
             3.3.2 Ether hydrolases
                3.3.2.8 limonene-1,2-epoxide hydrolase

Temperature Stability

Temperature Stability on EC 3.3.2.8 - limonene-1,2-epoxide hydrolase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41
wild-type LEH shows a thermostability of T50 30 (temperature at which 50% of enzyme activity is lost following a heat treatment for 30 min) = 41°C
49
-
15 min, 50% resiudal activity for wild-type
50
about, TM of enzyme Re-LEH is 50°C
74.5
apparent TM of enzyme Tomsk-LEH is 74.5°C
79.7
apparent TM of enzyme CH55-LEH is 79.7°C
additional information
R,R- and S,S-selective LEH variants (80-94% enantiomeric excess) show enhanced thermostability by 5-10°C compared to wild-type and still reasonable levels of activity