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3.3.2.1: isochorismatase

This is an abbreviated version!
For detailed information about isochorismatase, go to the full flat file.

Word Map on EC 3.3.2.1

Reaction

isochorismate
+
H2O
=
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate
 +
pyruvate

Synonyms

2,3 dihydro-2,3 dihydroxybenzoate synthase, 2,3-dihydro-2,3-dihydroxybenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoic synthase, AngB, AngB-ISC, EntB, IHL, IPL, ISC, ISOC2, isochorismatase, isochorismatase domain containing 2, isochorismatase hydrolase, isochorismate lyase, Mhpg, PchB, SOI1, VabB, VabB-ISC

ECTree

     3 Hydrolases
         3.3 Acting on ether bonds
             3.3.2 Ether hydrolases
                3.3.2.1 isochorismatase

Engineering

Engineering on EC 3.3.2.1 - isochorismatase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D227R
mutant constructed for functional analysis of EntB-EntE interaction
D240R
mutant constructed for functional analysis of EntB-EntE interaction
D244R
mutant constructed for functional analysis of EntB-EntE interaction
D263R
mutant constructed for functional analysis of EntB-EntE interaction
F264E
mutant constructed for functional analysis of EntB-EntE interaction
I239D
mutant constructed for functional analysis of EntB-EntE interaction
K269E
mutant constructed for functional analysis of EntB-EntE interaction
M249E
mutant constructed for functional analysis of EntB-EntE interaction
R247E
mutant constructed for functional analysis of EntB-EntE interaction
S245A
mutant constructed for functional analysis of EntB-EntE interaction
C118A
complete loss of activity
D9A
complete loss of activity
H51A
complete loss of activity
K84A
9% decrease in activity compared to wild-type
Q11A
20% decrease in activity compared to wild-type
Q91N
-
4fold reduction in kcat-value of enzymic activity, 10fold reduction in chorismate mutase activity
R54K
-
1000fold reduction in enzymic and in chorismate mutase activity
D35N
the mutation results in almost complete loss of enzyme activity
H34A
the mutant has about 80% of wild type activity
H34N
the mutant has about 35% of wild type activity
K118A
the mutant has about 30% of wild type activity
K118H
the mutation results in almost complete loss of enzyme activity
K118N
the mutation results in almost complete loss of enzyme activity
D35N
-
the mutation results in almost complete loss of enzyme activity
-
H34A
-
the mutant has about 80% of wild type activity
-
K118A
-
the mutant has about 30% of wild type activity
-
K118H
-
the mutation results in almost complete loss of enzyme activity
-
K118N
-
the mutation results in almost complete loss of enzyme activity
-