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3.3.2.1: isochorismatase

This is an abbreviated version!
For detailed information about isochorismatase, go to the full flat file.

Word Map on EC 3.3.2.1

Reaction

isochorismate
+
H2O
=
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate
+
pyruvate

Synonyms

2,3 dihydro-2,3 dihydroxybenzoate synthase, 2,3-dihydro-2,3-dihydroxybenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoic synthase, AngB, AngB-ISC, EntB, IHL, IPL, ISC, ISOC2, isochorismatase, isochorismatase domain containing 2, isochorismatase hydrolase, isochorismate lyase, Mhpg, PchB, SOI1, VabB, VabB-ISC

ECTree

     3 Hydrolases
         3.3 Acting on ether bonds
             3.3.2 Ether hydrolases
                3.3.2.1 isochorismatase

Cloned

Cloned on EC 3.3.2.1 - isochorismatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
angB gene, involved in anguibactin biosynthesis
-
expressed in Escherichia coli BL21(DE3) cells
gene angB-ISC encoding residues 1-216, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene vabB-ISC encoding residues 1-217, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
into the pGEM7z and the pET15bTEV vector for expression in Escherichia coli BL21DE3 cells
ISOC2 cDNA is cloned into the pECFP and pCMV-HA vector
-