3.3.2.1: isochorismatase
This is an abbreviated version!
For detailed information about isochorismatase, go to the full flat file.
Reaction
Synonyms
2,3 dihydro-2,3 dihydroxybenzoate synthase, 2,3-dihydro-2,3-dihydroxybenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoic synthase, AngB, AngB-ISC, EntB, IHL, IPL, ISC, ISOC2, isochorismatase, isochorismatase domain containing 2, isochorismatase hydrolase, isochorismate lyase, Mhpg, PchB, SOI1, VabB, VabB-ISC
ECTree
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Cloned
Cloned on EC 3.3.2.1 - isochorismatase
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gene angB-ISC encoding residues 1-216, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene vabB-ISC encoding residues 1-217, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
into the pGEM7z and the pET15bTEV vector for expression in Escherichia coli BL21DE3 cells