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3.2.2.9: adenosylhomocysteine nucleosidase

This is an abbreviated version!
For detailed information about adenosylhomocysteine nucleosidase, go to the full flat file.

Word Map on EC 3.2.2.9

Reaction

5'-deoxyadenosine
+
H2O
=
5-deoxy-D-ribose
 +
adenine

Synonyms

(adoHcy)/methylthioadenosine nucleosidase, 5'-dAdo nucleosidase, 5'-deoxyadenosine/5'-methylthioadenosine nucleosidase, 5'-methyladenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidases, 5'-methylthioadenosine/S-adenosylhomocysteine, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adenosylhomocysteine/methylthioadenosine nucleosidase, adoHcy/MeSAdo nucleosidase, adoHcy/MTA nucleosidase, Bgp, Bgp protein, BW246_00770, Cj0117, glycosaminoglycan-binding protein, K. pneumoniae MTA/SAH nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MKIGIIGA, MTA nucleosidase, MTA/AdoHcy nucleosidase, MTA/SAH nucleosidase, MTAN, Mtan-1, MTAN1, MTAN2, MTN1, MTN2, MtnN, nucleosidase, Pfs, Pfs protein, Pfs-2, Rv0091, S-adenosylhomocysteine nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase, SAHN

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.9 adenosylhomocysteine nucleosidase

Temperature Stability

Temperature Stability on EC 3.2.2.9 - adenosylhomocysteine nucleosidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 55
-
significant substrate conversion is seen even at 4°C. Activity is also seen at higher temperatures than 45°C, however the enzyme is rapidly inactivated (about 90%) after incubation at 55°C for 10 min
55
-
enzyme rapidly inactivated after 10 min at 55°C
66
Tm-value for mutant enzyme S196A and F105A
67
Tm-value for mutant enzymeV102A and Y107F
68
Tm-value for wild-type enzyme and mutant enzyme F151A
70
Tm-value for mutant enzymeS76A
71
Tm-value for mutant enzyme M173A and M9A
72
Tm-value for mutant enzyme R193A and F207A
74
the melting temperature is 74°C
75
Tm-value for mutant enzyme I50A