3.2.2.9: adenosylhomocysteine nucleosidase
This is an abbreviated version!
For detailed information about adenosylhomocysteine nucleosidase, go to the full flat file.
Word Map on EC 3.2.2.9
-
3.2.2.9
-
mtans
-
formycin
-
medicine
-
autoinducer-2
-
adomet-dependent
-
n-ribosidic
-
5\'-methylthiotubercidin
-
5-methylthioribose
-
analysis
-
drug development
- 3.2.2.9
-
mtans
- formycin
- medicine
- autoinducer-2
-
adomet-dependent
-
n-ribosidic
-
5\'-methylthiotubercidin
- 5-methylthioribose
- analysis
- drug development
Reaction
Synonyms
(adoHcy)/methylthioadenosine nucleosidase, 5'-dAdo nucleosidase, 5'-deoxyadenosine/5'-methylthioadenosine nucleosidase, 5'-methyladenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidases, 5'-methylthioadenosine/S-adenosylhomocysteine, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adenosylhomocysteine/methylthioadenosine nucleosidase, adoHcy/MeSAdo nucleosidase, adoHcy/MTA nucleosidase, Bgp, Bgp protein, BW246_00770, Cj0117, glycosaminoglycan-binding protein, K. pneumoniae MTA/SAH nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MKIGIIGA, MTA nucleosidase, MTA/AdoHcy nucleosidase, MTA/SAH nucleosidase, MTAN, Mtan-1, MTAN1, MTAN2, MTN1, MTN2, MtnN, nucleosidase, Pfs, Pfs protein, Pfs-2, Rv0091, S-adenosylhomocysteine nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase, SAHN
ECTree
3.2.2.9 adenosylhomocysteine nucleosidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.2.9 - adenosylhomocysteine nucleosidase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-deoxy-2'-fluoro-5'-deoxy-5'-(methylthio)adenosine + H2O
adenine + ?
-
F-MeSAdo, slow substrate
-
-
?
2'-deoxymethylthioadenosine + H2O
adenine + 2-deoxymethylthioribose
-
fairly good substrate
-
-
?
5'-butylthioadenosine + H2O
adenine + 5-butylthio-D-ribose
24% activity compared to 5'-methylthioadenosine
-
-
?
5'-chloroadenosine + H2O
adenine + 5-chloro-D-ribose
13% activity compared to 5'-methylthioadenosine
-
-
?
5'-iodoadenosine + H2O
adenine + 5-iodo-D-ribose
17% activity compared to 5'-methylthioadenosine
-
-
?
5'-isobutylthioadenosine + H2O
adenine + 5-isobutylthio-D-ribose
32% activity compared to 5'-methylthioadenosine
-
-
?
5'-isobutylthioadenosine + H2O
adenine + 5-isobutylthioribose
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 52%
-
-
?
5'-phenylthioadenosine + H2O
adenine + 5-phenylthio-D-ribose
39% activity compared to 5'-methylthioadenosine
-
-
?
adenosine + H2O
adenine + D-ribose
6% activity compared to 5'-methylthioadenosine
-
-
?
S-2-aza-adenosylhomocysteine + H2O
2-azaadenine + 5-(S-L-homocysteinyl)ribose
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 27%
-
-
?
S-adenosyl-D-homocysteine + H2O
adenine + 5-(S-D-homocysteinyl)ribose
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 26%
-
-
?
S-adenosylmethionine + H2O
adenine + hypoxanthine + methylthioribose + homoserine lactone
-
-
-
?
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
preferred substrate
-
-
ir
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
-
-
-
?
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
preferred substrate
-
-
ir
5'-ethylthioadenosine + H2O
adenine + 5-ethylthio-D-ribose
42% activity compared to 5'-methylthioadenosine
-
-
?
5'-ethylthioadenosine + H2O
adenine + 5-ethylthio-D-ribose
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 118%
-
-
?
5'-ethylthioadenosine + H2O
adenine + 5-ethylthio-D-ribose
Escherichia coli B / ATCC 11303
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 118%
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
100% activity
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
137135, 171850, 171851, 171852, 171854, 171856, 171858, 171859, 667625, 669334, 678158, 715849, 716263
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
Escherichia coli B / ATCC 11303
-
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
ir
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
ir
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
ir
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
?
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
-
-
-
-
?
5'-methylthioadenosine + H2O
?
-
-
-
-
?
5'-methylthioadenosine + H2O
?
-
-
-
-
?
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
-
-
-
-
?
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
-
-
-
-
?
5'-n-propylthioadenosine + H2O
adenine + 5-n-propylthioribose
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 106%
-
-
?
5'-n-propylthioadenosine + H2O
adenine + 5-n-propylthioribose
Escherichia coli B / ATCC 11303
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 106%
-
-
?
6-amino-6-deoxyfutalosine + H2O
dehypoxanthine futalosine + adenine
-
-
-
?
6-amino-6-deoxyfutalosine + H2O
dehypoxanthine futalosine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
?
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
?
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35%
-
-
?
S-adenosyl-L-homocysteine + H2O
?
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + 5-ribosyl-L-homocysteine
isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + 5-ribosyl-L-homocysteine
substrate of isozyme AtMTAN2, isozyme AtMTAN1 can bind but not hydrolyze S-adenosyl-L-homocysteine
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + 5-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
14% activity compared to 5'-methylthioadenosine
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
Pfs protein displays 76% activity and Bgp protein shows 84% relative activity towards S-adenosyl-L-homocysteine
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35%
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 42%
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
Escherichia coli B / ATCC 11303
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
Escherichia coli B / ATCC 11303
-
rate of 5'-methylthioadenosine hydrolysis taken as 100%, relative activity 35%
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
adenine + S-ribosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
the catalytic efficiency of the enzyme for S-adenosyl-L-homocysteine is only 0.004% of the value for S-methyl-5'-thioadenosine
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
42% activity compared to 5'-methylthioadenosine
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
ir
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
ir
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
ir
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
-
-
?
additional information
?
-
MTAN is dual-substrate specific and is responsible for the hydrolysis of 5'-methylthioadenosine and S-adenosylhomocysteine to adenine and their corresponding thioriboses
-
-
?
additional information
?
-
dynamics are an important factor in substrate selection in MTAN, overview. Constricted 5'-alkylthio binding site of isozymes AtMTAN1 and AtMTAN2, the isozymes are also active with S-methyl-5'-thioadenosine, EC 3.2.2.16, overview. Substrate binding induces a favourable entropic change in AtMTAN1. Usage of a xanthine oxidase-coupled spectrophotometric assay: for each molecule of adenine oxidized, two molecules of 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT) are reduced to formazan, a coloured product that absorbs at 470 nm
-
-
?
additional information
?
-
-
substrate specificities of isozymes, both isozymes are active with S-methyl-5'-thioadenosine, EC 3.2.2.16, overview
-
-
?
additional information
?
-
substrate specificities of isozymes, both isozymes are active with S-methyl-5'-thioadenosine, EC 3.2.2.16, overview
-
-
?
additional information
?
-
-
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is involved in quorum sensing, recycling 5'-methylthioadenosine from the polyamine pathway via adenine phosphoribosyltransferase and recycling 5-methylthioribose to methionine
-
-
?
additional information
?
-
Three structures along the reaction coordinate of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase are solved: Asp197Asn 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase/5'-methylthioadenosine complex, Glu12Gln 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase/5-methylthioribose/adenine complex, and wild-type 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase/glycerol complex. These structures provide insight into the conformational flexibility of the enzyme and nucleoside during catalysis
-
-
?
