3.2.2.9: adenosylhomocysteine nucleosidase

This is an abbreviated version!
For detailed information about adenosylhomocysteine nucleosidase, go to the full flat file.

Reaction

Synonyms

(adoHcy)/methylthioadenosine nucleosidase, 5'-dAdo nucleosidase, 5'-deoxyadenosine/5'-methylthioadenosine nucleosidase, 5'-methyladenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidases, 5'-methylthioadenosine/S-adenosylhomocysteine, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adenosylhomocysteine/methylthioadenosine nucleosidase, adoHcy/MeSAdo nucleosidase, adoHcy/MTA nucleosidase, Bgp, Bgp protein, BW246_00770, Cj0117, glycosaminoglycan-binding protein, K. pneumoniae MTA/SAH nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MKIGIIGA, MTA nucleosidase, MTA/AdoHcy nucleosidase, MTA/SAH nucleosidase, MTAN, Mtan-1, MTAN1, MTAN2, MTN1, MTN2, MtnN, nucleosidase, Pfs, Pfs protein, Pfs-2, Rv0091, S-adenosylhomocysteine nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase, SAHN

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.2 Hydrolysing N-glycosyl compounds

                3.2.2.9 adenosylhomocysteine nucleosidase

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Results	in table
1	Activating Compound
16542	AA Sequence
13	Application
1	CAS Registry Number
27	Cloned(Commentary)
23	Crystallization (Commentary)
14	General Information
2	General Stability
43	IC50 Value
157	Inhibitors
20	kcat/KM [mM/s]
108	Ki Value [mM]
79	KM Value [mM]
8	Localization
12	Molecular Weight [Da]
87	Natural Substrates/ Products (Substrates)
455	Organism
11	Pathway
131	PDB ID
13	pH Optimum
4	pH Range
42	Protein Variants
30	Purification (Commentary)
6	Reaction
1	Reaction Type
84	Reference
4	Source Tissue
12	Specific Activity [micromol/min/mg]
4	Storage Stability
205	Substrates and Products (Substrate)
20	Subunits
132	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Range [°C]
12	Temperature Stability [°C]
65	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.2.2.9 - adenosylhomocysteine nucleosidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°, repeatedly frozen and thawed without loss of activity over a 9 months period, upon storage at 4°C only 50% of activity retained after 3 weeks

Escherichia coli

-

171851

-20°C, 50 mM potassium phosphate buffer (pH 7.0), at least 2 months, no significant loss of activity

Escherichia coli

-

171854

0°C, 0.1 M phosphate buffer containing 0.009 M 2-mercaptoethanol and 0.0005 M EDTA stable for 2 weeks, retains 80% of its total activity after 2 weeks at -40°C

Escherichia coli

-

171852

0°C, loses 50% activity in 48 h, -20°C very unstable, lost nearly all activity when stored for 1 week

Escherichia coli

-

171850