3.2.2.9: adenosylhomocysteine nucleosidase

This is an abbreviated version!
For detailed information about adenosylhomocysteine nucleosidase, go to the full flat file.

Word Map on EC 3.2.2.9

3.2.2.9

mtans

formycin

medicine

autoinducer-2

adomet-dependent

n-ribosidic

5\'-methylthiotubercidin

5-methylthioribose

analysis

drug development

Reaction

Synonyms

(adoHcy)/methylthioadenosine nucleosidase, 5'-dAdo nucleosidase, 5'-deoxyadenosine/5'-methylthioadenosine nucleosidase, 5'-methyladenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidases, 5'-methylthioadenosine/S-adenosylhomocysteine, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adenosylhomocysteine/methylthioadenosine nucleosidase, adoHcy/MeSAdo nucleosidase, adoHcy/MTA nucleosidase, Bgp, Bgp protein, BW246_00770, Cj0117, glycosaminoglycan-binding protein, K. pneumoniae MTA/SAH nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MKIGIIGA, MTA nucleosidase, MTA/AdoHcy nucleosidase, MTA/SAH nucleosidase, MTAN, Mtan-1, MTAN1, MTAN2, MTN1, MTN2, MtnN, nucleosidase, Pfs, Pfs protein, Pfs-2, Rv0091, S-adenosylhomocysteine nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase, SAHN

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.2 Hydrolysing N-glycosyl compounds

3.2.2.9 adenosylhomocysteine nucleosidase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
1	Activating Compound
16542	AA Sequence
13	Application
1	CAS Registry Number
27	Cloned(Commentary)
23	Crystallization (Commentary)
14	General Information
2	General Stability
43	IC50 Value
157	Inhibitors
20	kcat/KM [mM/s]
108	Ki Value [mM]
79	KM Value [mM]
8	Localization
12	Molecular Weight [Da]
87	Natural Substrates/ Products (Substrates)
455	Organism
11	Pathway
131	PDB ID
13	pH Optimum
4	pH Range
42	Protein Variants
30	Purification (Commentary)
6	Reaction
1	Reaction Type
84	Reference
4	Source Tissue
12	Specific Activity [micromol/min/mg]
4	Storage Stability
205	Substrates and Products (Substrate)
20	Subunits
132	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Range [°C]
12	Temperature Stability [°C]
65	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 3.2.2.9 - adenosylhomocysteine nucleosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Purification (commentary) Search

160-fold

220-fold

30-fold

9800-fold

ammonium sulfate precipitation, DEAE-Sephadex gel filtration, hydroxyapatite column chromatography, S-formycinylhomocysteine-Sepharose column chromatography, and Sephacryl S-200 gel filtration

Escherichia coli

171854

glutathione column chromatography, HiTrap Q Sepharose column chromatography, and Superose 12 gel filtration

Arabidopsis thaliana

Q7XA67

702045

HisTrap column chromatography and Superdex 200 gel filtration

2 entries

Ni-NTA affinity column chromatography and gel filtration

Arabidopsis thaliana

Q9T0I8

709718

Ni-NTA agarose column chromatography

Arabidopsis thaliana

Q9T0I8

681477

Ni-NTA column chromatography

Escherichia coli

715849

Ni-NTA column chromatography and Superdex 200 gel filtration

Staphylococcus aureus

727044

Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration

2 entries

Ni-Sepharose column chromatography

Campylobacter jejuni

Q0PC20

715607

nickel affinity column chromatography and Superdex 200 gel filtration

Brucella melitensis

726889

recombinant His-tagged wild-type and mutant isozymes AtMTAN1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Arabidopsis thaliana

Q9T0I8

709718

recombinant isozyme AtMTAN2 from Escherichia coli to homogeneity

recombinant protein

recombinant wild-type and mutant His-tagged MTAN from strain BL21(DE3) by nickel affinity chromatography

Escherichia coli

709718

rMTAN, recombinant MTA/SAH nucleosidase

Escherichia coli

171857