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3.2.2.9: adenosylhomocysteine nucleosidase

This is an abbreviated version!
For detailed information about adenosylhomocysteine nucleosidase, go to the full flat file.

Word Map on EC 3.2.2.9

Reaction

5'-deoxyadenosine
+
H2O
=
5-deoxy-D-ribose
+
adenine

Synonyms

(adoHcy)/methylthioadenosine nucleosidase, 5'-dAdo nucleosidase, 5'-deoxyadenosine/5'-methylthioadenosine nucleosidase, 5'-methyladenosine nucleosidase, 5'-methylthioadenosine nucleosidase, 5'-methylthioadenosine nucleosidases, 5'-methylthioadenosine/S-adenosylhomocysteine, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2, 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, adenosylhomocysteine/methylthioadenosine nucleosidase, adoHcy/MeSAdo nucleosidase, adoHcy/MTA nucleosidase, Bgp, Bgp protein, BW246_00770, Cj0117, glycosaminoglycan-binding protein, K. pneumoniae MTA/SAH nucleosidase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MKIGIIGA, MTA nucleosidase, MTA/AdoHcy nucleosidase, MTA/SAH nucleosidase, MTAN, Mtan-1, MTAN1, MTAN2, MTN1, MTN2, MtnN, nucleosidase, Pfs, Pfs protein, Pfs-2, Rv0091, S-adenosylhomocysteine nucleosidase, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase, SAHN

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.9 adenosylhomocysteine nucleosidase

Cloned

Cloned on EC 3.2.2.9 - adenosylhomocysteine nucleosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged recombinant protein
-
expressed in Escherichia coli as His-tagged protein
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 Codon + cells
expressed in Escherichia coli BL21 Codon+ cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) RIL cells
expressed in Escherichia coli BL21(DE3) Rosetta cells
-
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli Top10F' cells
-
expression in Escherichia coli
expression with His-tag
-
gene encoding the enzyme cloned and recombinant MTA/SAH nucleosidase rMTAN expressed
-
isozyme AtMTAN1, overexpression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3)
isozyme AtMTAN2, DNA and amino acid sequence determination and analysis, overexpression of isozyme AtMTAN2 in Escherichia coli
overexpression of wild-type and mutant His-tagged enzyme in strain BL21(DE3)
-
pfs gene cloned and expressed in pCRII using PCR, subcloned into pAL781
-