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3.2.2.5: NAD+ glycohydrolase

This is an abbreviated version!
For detailed information about NAD+ glycohydrolase, go to the full flat file.

Word Map on EC 3.2.2.5

Reaction

NAD+
+
H2O
=
ADP-D-ribose
+
nicotinamide
+
H+

Synonyms

(Streptococcus pyogenes NAD+ glycohydrolase), AA-NADase, Acute lymphoblastic leukemia cells antigen CD38, ADP-ribosyl cyclase/NAD+-glycohydrolase, ADPRC, ADRC, Antigen BP3, beta-NAD+ glycohydrolase, beta-NAD+glycohydrolase, BP-3 alloantigen, cADPr hydrolase, CagL, CD157, CD157 antigen, CD38, CD38 homolog, CD38 like activity, CD38H, co-toxin NADase, Cyclic ADP-ribose hydrolase, diphosphopyridine nucleosidase, diphosphopyridine nucleotidase, DPN hydrolase, DPNase, ecto-NAD-glycohydrolase, HvnA, HvnB, I-19, Lymphocyte differentiation antigen CD38, NACE, NAD glycohydrolase, NAD hydrolase, NAD nucleosidase, NAD(+) nucleosidase, NAD+ catabolizing enzyme, NAD+ glycohydrolase, NAD+-glycohydrolase, NAD+glycohydrolase, NAD-glycohydrolase, NADase, Nga, nicotinamide adenine dinucleotide glycohydrolase, nicotinamide adenine dinucleotide nucleosidase, NIM-R5 antigen, PA0093, PAAR motif containing protein, PFL_6209, pNADase, SmNACE, sNADase, SPN, T10, Tse6

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.5 NAD+ glycohydrolase

Purification

Purification on EC 3.2.2.5 - NAD+ glycohydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
15.6fold to homogeneity, rapid 3-step chromatographic procedure
-
Blue Sepharose column chromatography, gel filtration
-
from seminal plasma
-
method for purification of enzymatically active NADase fused to an amino-terminal fragment of anthrax toxin lethal factor
native enzyme
-
native enzyme 81fold from reticulocytes, by lipid extracction, affinity and hydrophobic interaction chromatography, and gel filtration
-
native enzyme from venom
-
native enzymes partially from culture supernatant, recombinant enzymes from Escherichia coli
partial
-
partially, solubilization from the membrane fraction, best with CHAPS or Triton X-100
-
particulate pNADase from brain, solubilization to give soluble sNADase
-
recombinant C-terminally HA-tagged and His6-tagged enzyme variant SPNJ4 from Escherichia coli strain TOP10
-
recombinant His-tagged wild-type and E391Q mutant SPN from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Pichia apstoris culture supernatant by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration
Talon metal affinity column chromatography, Q-Sepharose column chromatography, and S-200 gel filtration
-
wild-type and mutant enzymes by anion exchange chromatography