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3.2.2.5: NAD+ glycohydrolase

This is an abbreviated version!
For detailed information about NAD+ glycohydrolase, go to the full flat file.

Word Map on EC 3.2.2.5

Reaction

NAD+
+
H2O
=
ADP-D-ribose
 +
nicotinamide
 +
H+

Synonyms

(Streptococcus pyogenes NAD+ glycohydrolase), AA-NADase, Acute lymphoblastic leukemia cells antigen CD38, ADP-ribosyl cyclase/NAD+-glycohydrolase, ADPRC, ADRC, Antigen BP3, beta-NAD+ glycohydrolase, beta-NAD+glycohydrolase, BP-3 alloantigen, cADPr hydrolase, CagL, CD157, CD157 antigen, CD38, CD38 homolog, CD38 like activity, CD38H, co-toxin NADase, Cyclic ADP-ribose hydrolase, diphosphopyridine nucleosidase, diphosphopyridine nucleotidase, DPN hydrolase, DPNase, ecto-NAD-glycohydrolase, HvnA, HvnB, I-19, Lymphocyte differentiation antigen CD38, NACE, NAD glycohydrolase, NAD hydrolase, NAD nucleosidase, NAD(+) nucleosidase, NAD+ catabolizing enzyme, NAD+ glycohydrolase, NAD+-glycohydrolase, NAD+glycohydrolase, NAD-glycohydrolase, NADase, Nga, nicotinamide adenine dinucleotide glycohydrolase, nicotinamide adenine dinucleotide nucleosidase, NIM-R5 antigen, PA0093, PAAR motif containing protein, PFL_6209, pNADase, SmNACE, sNADase, SPN, T10, Tse6

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.5 NAD+ glycohydrolase

Inhibitors

Inhibitors on EC 3.2.2.5 - NAD+ glycohydrolase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-deoxy-2'-fluoroarabinoside adenine dinucleotide
-
-
2-Hydroxy-3,5-diiodo-benzoic acid
-
-
2-Hydroxy-5-iodo-benzoic acid
-
-
3-acetylpyridine
3-aminobenzamide
-
-
4-Carboxyhydrazide-pyridine-adenine dinucleotide
-
-
8-azidoadenosyl-carba-NAD+
-
-
8-azidoadenosyl-pseudocarba-NAD+
-
-
adenosine
-
-
adenosine 3',5'-monophosphate
ADP-(2-deoxy-2-fluoro-D-arabinose)
the NAD analogue forms a covalent adduct after nicotinamide cleavage, resulting in inhibition of the enzyme activity, binding structure, overview
ADP-(2-deoxy-2-fluoro-D-ribose)
the NAD analogue forms a covalent adduct after nicotinamide cleavage, resulting in inhibition of the enzyme activity, binding structure, overview
ADP-ribose
ADP-ribosylated protein
-
-
-
ADPribose
Alpha-NAD+
-
5 mM, 70% inhibition
ara-NAD+
-
-
b-series gangliosides
-
-
-
beta-NAD+
-
substrate inhibition, mediated by ADP-ribosylation, partially reversible by arginine or histidine, inhibits apoptosis in vivo
beta-NADP+
-
in vivo
carba-NAD+
-
-
Cu2+
-
the enzyme has two classes of Cu2+ binding sites, one activator site with high affinity and approximately six inhibitor sites with low affinity
cyanidin
deamino-NAD+
-
5 mM, almost complete inhibition
delphinidin
-
-
diosmetinidin
-
-
dioxane
-
more than 0.05 mM inhibit the activity
dithiothreitol
-
inhibits both NADase and ADPase activities through the reduction of Cu(II) to Cu(I) and the cleavage of disulfide-bonds in AA-NADase
fisetinidin
-
-
gangliosides
-
-
-
GD1a
-
exogenous ganglioside, slight inhibition, inhibition in vivo
GD1b
-
exogenous ganglioside, inhibition in vivo
GD3
-
exogenous ganglioside, inhibition in vivo
GDP-ribose
-
-
glutathione
-
inhibits both NADase and ADPase activities through the reduction of Cu(II) to Cu(I) and the cleavage of disulfide-bonds in AA-NADase
GM1b
-
exogenous ganglioside, slight inhibition, inhibition in vivo
GT1b
-
exogenous ganglioside, inhibition in vivo, needs to be incorporated into the cell to inhibit CD38, preferably cis interaction, i.e. CD38 and GT1b are located on the same cell, the tandem sialic acid residues linked to the internal galactose of the gangliotetraose core are crucial to the inhibition
hydroxylamine
inhibits the reaction between ADP-ribose and polypeptides
iodoacetamide
-
-
isoniazid
kuromanin
-
-
L-ascorbate
-
inhibits AA-NADase on both NADase and ADPase activities through the reduction of Cu(II) in AA-NADase to Cu(I)
luteolinidin
-
-
m-aminophenylboronic acid
-
-
monoclonal antibody CS/2
-
inhibits the NAD+ glycohydolase activity of both the isolated extracellular domain of CD38 and cell-surface CD38, non-competitive. No effect on cyclase activity of enzyme
-
myricetin
-
-
N1-cyclic inosine diphosphate ribose
-
NADP+
-
5 mM, almost complete inhibition
nicotinamide
nicotinic acid
NMN
-
5 mM, 70% inhibition
pelargonidin
-
-
petunidin
-
-
Procion Blue HB
-
-
protein IFS
-
pseudocarba-NAD+
quercetagetin
-
-
quercetagetinidin
-
-
quercetin
-
-
rac-taxifolin
-
-
robinetin
-
-
salicylic acid
-
-
Streptococcal NAD glycohydrolase inhibitor
the ifs gene, or SpyM3_0129, encodes an endogenous competitive inhibitor for the enzyme. IFS is localized intracellularly and forms a complex with the enzyme. This intracellular complex must be dissociated during export through the cell envelope. The interface between SPNct and IFS is highly rich in water molecules, binding structure, overview
-
streptococcal NADase inhibitor
-
161 amino acids, MW 18800 Da, thermostable. Monomeric NADase and the streptococcal NADase inhibitor rapidly form in vitro a stable heterodimer complex in the ratio 1.1, resulting in complete suppression of the hydrolase activity. The inhibitor protein, coexpressed and complexed with NADase, may protect the producer cocci from exhausion of NAD
-
thioNAD+
tris(2-carboxyethyl)phosphine
-
TCEP, inhibits both NADase and ADPase activities through the reduction of Cu(II) to Cu(I) and the cleavage of disulfide-bonds in AA-NADase
additional information
-