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3.2.2.5: NAD+ glycohydrolase

This is an abbreviated version!
For detailed information about NAD+ glycohydrolase, go to the full flat file.

Word Map on EC 3.2.2.5

Reaction

NAD+
+
H2O
=
ADP-D-ribose
 +
nicotinamide
 +
H+

Synonyms

(Streptococcus pyogenes NAD+ glycohydrolase), AA-NADase, Acute lymphoblastic leukemia cells antigen CD38, ADP-ribosyl cyclase/NAD+-glycohydrolase, ADPRC, ADRC, Antigen BP3, beta-NAD+ glycohydrolase, beta-NAD+glycohydrolase, BP-3 alloantigen, cADPr hydrolase, CagL, CD157, CD157 antigen, CD38, CD38 homolog, CD38 like activity, CD38H, co-toxin NADase, Cyclic ADP-ribose hydrolase, diphosphopyridine nucleosidase, diphosphopyridine nucleotidase, DPN hydrolase, DPNase, ecto-NAD-glycohydrolase, HvnA, HvnB, I-19, Lymphocyte differentiation antigen CD38, NACE, NAD glycohydrolase, NAD hydrolase, NAD nucleosidase, NAD(+) nucleosidase, NAD+ catabolizing enzyme, NAD+ glycohydrolase, NAD+-glycohydrolase, NAD+glycohydrolase, NAD-glycohydrolase, NADase, Nga, nicotinamide adenine dinucleotide glycohydrolase, nicotinamide adenine dinucleotide nucleosidase, NIM-R5 antigen, PA0093, PAAR motif containing protein, PFL_6209, pNADase, SmNACE, sNADase, SPN, T10, Tse6

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.5 NAD+ glycohydrolase

Cloned

Cloned on EC 3.2.2.5 - NAD+ glycohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of inactive enzyme variant SPNH5 from strain HSC5 and of active variant SPNJ4, expression of C-terminally HA-tagged and His6-tagged variant SPNJ4 in Escherichia coli strain TOP10
-
co-expression of N-terminally His6-tagged enzyme with inhibitor IFS in Escherichia coli strain Rosetta2 (DE3)
DNA and amino acid sequence determination and analysis, genomic structure, recombinant expression of FLAG-tagged wild-type and mutant enzymes in HEK293 cells
-
enzymatic domain of CD38
-
expressed in Pichia pastoris
-
expression in Escherichia coli, FLAG-tag
-
expression in THP-1 cells, selection of transfected cells by G418
-
expression of extracellular region of CD38 in Drosophila S2 cells
-
expression of extramembrane domain in Saccharomyces cerevisiae
-
expression of wild-type and mutant enzymes in Pichia pastoris
gene hvnA, part of a four gene operon, and monocistronic gene hvnB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha and BW23474
gene nga, cloning n Escherichia coli strain JM109, expression of wild-type and mutant enzymes
-
genotyping, SPN has a different pattern of polymorphisms than Streptococcus pyogenes housekeeping genes. The ratio of nonsynonymous to synonymous nucleotide polymorphisms reveals different patterns of evolution for NADase-active and NADase-inactive strains. SPN amino acid sequence remains preserved despite loss of NADase activity, overview
-
recombinant expression of His-tagged wild-type and E391Q mutant SPN in Escherichia coli as stable and soluble monomer in the periplasmic space
-
recombinant expression of His-tagged wild-type and mutant enzymes in Pichia apstoris and secretion to the culture medium
the C-terminal glycohydrolase domain of SPN (residues 191-451) in complex with IFS is expressed in Escherichia coli strain DL41
-