3.2.2.30: aminodeoxyfutalosine nucleosidase

This is an abbreviated version!
For detailed information about aminodeoxyfutalosine nucleosidase, go to the full flat file.

Reaction

Synonyms

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2, 6-amino-6-deoxyfutalosine hydrolase, adoHcy/MTA nucleosidase, AFL nucleosidase, aminofutalosine nucleosidase, Cj0117, futalosine hydrolase, methylthioadenosine nucleosidase, methylthioadenosine/S-adenosylhomocysteine nucleosidase, MqnB, MTAN, MTAN2, MtnN, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.2 Hydrolysing N-glycosyl compounds

3.2.2.30 aminodeoxyfutalosine nucleosidase

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Results	in table
16	AA Sequence
7	Cloned(Commentary)
6	Crystallization (Commentary)
2	General Information
14	IC50 Value
52	Inhibitors
5	kcat/KM [mM/s]
41	Ki Value [mM]
6	KM Value [mM]
1	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
16	Organism
6	Pathway
9	PDB ID
2	pH Optimum
10	Protein Variants
9	Purification (Commentary)
1	Reaction
16	Reference
7	Specific Activity [micromol/min/mg]
1	Storage Stability
39	Substrates and Products (Substrate)
4	Subunits
31	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 3.2.2.30 - aminodeoxyfutalosine nucleosidase

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Campylobacter jejuni

Q0PC20

715607

ammonium sulfate precipitation, DEAE-Sephadex gel filtration, hydroxyapatite column chromatography, S-formycinylhomocysteine-Sepharose column chromatography, and Sephacryl S-200 gel filtration

Escherichia coli

171854

HisTrap column chromatography

Helicobacter pylori

O24915

726595

HisTrap column chromatography and Superdex 200 gel filtration

2 entries

HisTrap column chromatography and Superdex 75 gel filtration

Helicobacter pylori

O24915

726595

Ni-NTA affinity column chromatography and gel filtration

Escherichia coli

P0AF12

709718

Ni-NTA agarose column chromatography

Arabidopsis thaliana

681477

Ni-NTA column chromatography and Superdex 200 gel filtration

Staphylococcus aureus

727044