3.2.2.29: thymine-DNA glycosylase

This is an abbreviated version!
For detailed information about thymine-DNA glycosylase, go to the full flat file.

Word Map on EC 3.2.2.29

3.2.2.29

demethylation

5-methylcytosine

cytosine

deamination

glycosylases

5-carboxylcytosine

mispairs

5-formylcytosine

5-hydroxymethylcytosine

ten-eleven

excises

uracil-dna

abasic

methyl-cpg

3,n4-ethenocytosine

tdg-mediated

5-hydroxymethyluracil

sumo-1

base-excision

methyl-cpg-binding

medicine

Reaction

Hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free thymine. =

Synonyms

G/T glycosylase, G:T mismatch-specific thymine DNA-glycosylase, hsTDG, hTDG, mismatch-specific thymine-DNA glycosylase, mismatch-specific thymine-DNA N-glycosylase, More, Pa-MIG, T/U mismatch DNA glycosylase, T:G mismatch-specific thymidine-DNA glycosylase, TDG, Thd1p, thymine DNA glycosylase, thymine DNA-glycosylase, thymine-DNA glycosylase, uracil/thymine DNA glycosylase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.2 Hydrolysing N-glycosyl compounds

3.2.2.29 thymine-DNA glycosylase

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Results	in table
6	Activating Compound
9	AA Sequence
2	Application
1	CAS Registry Number
22	Cloned(Commentary)
10	Crystallization (Commentary)
53	Disease/ Diagnostics
2	Expression
15	General Information
13	Inhibitors
4	KM Value [mM]
9	Localization
2	Metals/Ions
7	Molecular Weight [Da]
60	Natural Substrates/ Products (Substrates)
53	Organism
21	PDB ID
4	pH Optimum
1	pH Range
1	pI Value
4	Posttranslational Modification
22	Protein Variants
21	Purification (Commentary)
1	Reaction
53	Reference
36	Source Tissue
4	Specific Activity [micromol/min/mg]
130	Substrates and Products (Substrate)
9	Subunits
99	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
9	Turnover Number [1/s]

Purification

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Purification on EC 3.2.2.29 - thymine-DNA glycosylase

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9 entries

cDNA sequence encoding human TDG (residues 112339) is subcloned into a pGEX4T-3 vector. SUMO-3-modified TDG112339 (SUMO-3TDG) is expressed bacterially using an Escherichia coli SUMOylation system by co-transforming the Escherichia coli strain BL21(DE3) with TDG112339/pGEX4T-3 and pT-E1E2S2 protein expression vectors

Homo sapiens

Q13569

693615

glutathione Sepharose column chromatography

Homo sapiens

731862

GSTrap column chromatography

Homo sapiens

Q13569

750696

HiTrap Q column chromatography

Homo sapiens

Q13569

749910

Mono Q column chromatography as final chromatographic step

Homo sapiens

Q13569

751751

nickel-charged chelating column chromatography, HiTrap SP column chromatography, and Superdex 75 gel filtration

recombinant

recombinant enzyme from NIH3T3 fibroblasts

Mus musculus

710145

recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography

Aeropyrum pernix

710481

recombinant His-tagged TDG from Escherichia coli strain BL21 (DE3)

Homo sapiens

Q13569

694421

SP Sepharose column chromatography

Homo sapiens

Q13569

751726