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3.2.2.29: thymine-DNA glycosylase

This is an abbreviated version!
For detailed information about thymine-DNA glycosylase, go to the full flat file.

Word Map on EC 3.2.2.29

Reaction

Hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free thymine. =

Synonyms

G/T glycosylase, G:T mismatch-specific thymine DNA-glycosylase, hsTDG, hTDG, mismatch-specific thymine-DNA glycosylase, mismatch-specific thymine-DNA N-glycosylase, More, Pa-MIG, T/U mismatch DNA glycosylase, T:G mismatch-specific thymidine-DNA glycosylase, TDG, Thd1p, thymine DNA glycosylase, thymine DNA-glycosylase, thymine-DNA glycosylase, uracil/thymine DNA glycosylase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.29 thymine-DNA glycosylase

Crystallization

Crystallization on EC 3.2.2.29 - thymine-DNA glycosylase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of hTDG (catalytic domain, hTDGcat) in complex with abasic DNA, at 2.8 A resolution. The enzyme crystallizes in a 2:1 complex with DNA, one subunit bound at the abasic site, and the other at an undamaged (nonspecific) site
crystals of SUMO-1–TDG complex are grown in 25% PEG 3350, 0.2 M MgCl2 and 0.1 M Tris-HCl (pH 8.5) at 20°C by using a micro-seeding technique. Crystal structure of the central region of human TDG conjugated to SUMO-1 at 2.1 A resolution
crystals of SUMO-3–TDG are grown in 1.5 M sodium malonate (pH 5.0) at 20°C, by using a streaking technique. Crystal structure of the central region of TDG conjugated to SUMO-3
enzyme bound to DNA with a non-cleavable (2'-fluoroarabino) analog of 5-formyldeoxycytidine flipped into its active site
enzyme construct TDG82-308, sitting drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 6.0
free enzyme and in complex with DNA, sitting drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 6.0
in complex with a 28-base pair DNA containing a guanine:5-hydroxymethyluracil mismatch, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6
in complex with duplex DNA containing either 5-carboxylcytosine or a 1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-carbonylcytosine, hanging drop vapor diffusion method, using
mutant N140A in complex with guanine:5-carboxylcytosine containing DNA, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6
structural basis of substrate specificity