3.2.2.24: ADP-ribosyl-[dinitrogen reductase] hydrolase
This is an abbreviated version!
For detailed information about ADP-ribosyl-[dinitrogen reductase] hydrolase, go to the full flat file.
Word Map on EC 3.2.2.24
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3.2.2.24
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adp-ribosylation
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rhodospirillum
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rubrum
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adp-ribosyltransferase
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azospirillum
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brasilense
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adp-ribose
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pii
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macrod2
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mono-adp-ribosylation
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dratg
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fe-protein
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lipoferum
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glnz
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macrodomains
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switch-off
- 3.2.2.24
-
adp-ribosylation
- rhodospirillum
- rubrum
- adp-ribosyltransferase
- azospirillum
- brasilense
- adp-ribose
- pii
- macrod2
-
mono-adp-ribosylation
-
dratg
- fe-protein
- lipoferum
- glnz
-
macrodomains
-
switch-off
Reaction
Synonyms
ADP-ribosyl glycohydrolase, ADP-ribosylglycohydrolase, ADP-ribosylhydrolase, azoferredoxin-activating enzymes, dinitrogenase reductase activating glycohydrolase, dinitrogenase reductase ADP-glycohydrolase, dinitrogenase reductase glycohydrolase, dinitrogenase reductase-activating glycohydrolase, DRAG, glycosidase, azoferredoxin, mono-ADP-ribosylhydrolase, More
ECTree
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Crystallization
Crystallization on EC 3.2.2.24 - ADP-ribosyl-[dinitrogen reductase] hydrolase
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hanging drop vapor diffusion method, using 35% (v/v) glycerol ethoxylate and 0.2 M lithium citrate
recombinant DraG bound to substrate Azospirillum brasilense dinitrogenase, mixing of protein solution with reservoir solution, containing 0.1M HEPES, pH 7.5, 15% w/v PEG 20000, in a 1:1 ratio,2-3 days X-ray diffraction structure determination and analysis at 2.5 resolution, modelling
purified recombinant DraG in the holo and ADP-ribose bound forms and a reaction intermediate analogue, sitting drop vapor diffusion method, 0.0006 ml of of 10 mg/ml wild-type DraG is mixed with an equal volume of 15% PEG 3350, 0.3 M NH4Cl, and 0.1 M Tris, pH 7.0, several days, cryoprotection in 20% PEG 3350, 0.3 M NH4Cl, 0.1M Tris, pH 7.0, and 20% glycerol, X-ray diffraction structure determination and analysis at 2.2 A resolution, modelling, overview