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3.2.2.24: ADP-ribosyl-[dinitrogen reductase] hydrolase

This is an abbreviated version!
For detailed information about ADP-ribosyl-[dinitrogen reductase] hydrolase, go to the full flat file.

Word Map on EC 3.2.2.24

Reaction

[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine
=
ADP-D-ribose
+
[dinitrogen reductase]-L-arginine

Synonyms

ADP-ribosyl glycohydrolase, ADP-ribosylglycohydrolase, ADP-ribosylhydrolase, azoferredoxin-activating enzymes, dinitrogenase reductase activating glycohydrolase, dinitrogenase reductase ADP-glycohydrolase, dinitrogenase reductase glycohydrolase, dinitrogenase reductase-activating glycohydrolase, DRAG, glycosidase, azoferredoxin, mono-ADP-ribosylhydrolase, More

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.24 ADP-ribosyl-[dinitrogen reductase] hydrolase

Crystallization

Crystallization on EC 3.2.2.24 - ADP-ribosyl-[dinitrogen reductase] hydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 35% (v/v) glycerol ethoxylate and 0.2 M lithium citrate
recombinant DraG bound to substrate Azospirillum brasilense dinitrogenase, mixing of protein solution with reservoir solution, containing 0.1M HEPES, pH 7.5, 15% w/v PEG 20000, in a 1:1 ratio,2-3 days X-ray diffraction structure determination and analysis at 2.5 resolution, modelling
purified recombinant DraG in the holo and ADP-ribose bound forms and a reaction intermediate analogue, sitting drop vapor diffusion method, 0.0006 ml of of 10 mg/ml wild-type DraG is mixed with an equal volume of 15% PEG 3350, 0.3 M NH4Cl, and 0.1 M Tris, pH 7.0, several days, cryoprotection in 20% PEG 3350, 0.3 M NH4Cl, 0.1M Tris, pH 7.0, and 20% glycerol, X-ray diffraction structure determination and analysis at 2.2 A resolution, modelling, overview