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3.2.2.21: DNA-3-methyladenine glycosylase II

This is an abbreviated version!
For detailed information about DNA-3-methyladenine glycosylase II, go to the full flat file.

Word Map on EC 3.2.2.21

Reaction

7-methyladenine-containing DNA
+
H2O
=
7-Methyladenine
+
deadenylated DNA

Synonyms

3-methyladenine DANN glycosylase II, 3-methyladenine DNA glycosylase II, 3-methyladenine-DNA glycosidase II, 3-methyladenine-DNA glycosylase II, AAG, ALK, AlkA, AlkA protein, AlkA1, AlkA2, AlkD, alkylpurine-DNA-N-glycosylase, ANPG, deoxyribonucleate 3-methyladenine glycosidase II, DNA glycosylase, DNA-3-methyladenine glycosidase II, helix-hairpin-helix DNA glycosylase, m3A DNA glycosylase II, MAG, Mag1 protein, MPG, N-methylpurine-DNA glycosylase, TagII

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.21 DNA-3-methyladenine glycosylase II

Cloned

Cloned on EC 3.2.2.21 - DNA-3-methyladenine glycosylase II

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alkAg+ gene cloned and sequenced
-
cloned and expressed in Escherichia coli BL21(DE3)
-
construction of recombinant GST-MPG fusion proteins
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon Plus cells
expressed in Escherichia coli HMS174 cells
-
expression by adenoviral system and targeting of enzyme to mitochondria or nucleus of primary astrocytes
-
expression in Escherichia coli BL21, AlkD
-
gene afalkA, AF2117 ORF cloned and overexpressed in Escherichia coli, gene function suppresses the alkylation sensitivity in Escherichia coli tag alkA double mutants
-
MPG deletion mutant N-DELTA100C-DELTA18 overexpressed in Escherichia coli
-
overexpressed in MDA-MB231 breast cancer cell line
-
truncated MPG polypeptides expressed in Escherichia coli using vector pRSET, full length MPG protein cloned in eukaryotic baculovirus expression vector pVL1393 and host Sf9 insect cells used for expression
-