3.2.2.20: DNA-3-methyladenine glycosylase I
This is an abbreviated version!
For detailed information about DNA-3-methyladenine glycosylase I, go to the full flat file.
Word Map on EC 3.2.2.20
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3.2.2.20
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glycosylases
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abasic
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excises
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self-labeling
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1,n6-ethenoadenine
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snap-tagged
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3-methylguanine
- 3.2.2.20
- glycosylases
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abasic
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excises
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self-labeling
- 1,n6-ethenoadenine
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snap-tagged
- 3-methylguanine
Reaction
Synonyms
3-MeA DNA glycosylase I, 3-methyl adenine DNA glycosylase I, 3-methyladenine DNA glycosylase, 3-methyladenine DNA glycosylase I, 3MeA, 3MeA DNA glycosylase, AAG, Aag 3MeA DNA glycosylase, AlkC, AlkD, APNG, APNG DNA glycosylase, bAag, deoxyribonucleate 3-methyladenine DNA glycosidase I, DNA-3-methyladenine DNA glycosidase I, GI, human m3A DNA glycosylase, m3A DNA glycosylase I, MAG, MAG1, Mag1 3-methyladenine DNA glycosylase, Mag1p, Mag2p, MPG, MpgI, N3-methyladenine DNA glycosylase, protein Tag, TAG, TagA, TagA protein, TagI, Yx1J
ECTree
3.2.2.20 DNA-3-methyladenine glycosylase IAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.2.20 - DNA-3-methyladenine glycosylase I
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5'-CGATAGCATCCT[hypoxanthine]CCTTCTCTCCAT-3' + H2O
?
-
-
-
-
?
alkylated DNA + H2O
3-methyladenine + 3-methylguanine + ?
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AlkC is specific for 3-methylpurines
-
-
?
3-methyladenine residues in alkylated DNA + H2O
3-methyladenine + ?
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-
-
-
?
3-methyladenine residues in alkylated DNA + H2O
3-methyladenine + ?
-
-
-
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
calf thymus DNA
prefers to release hypoxanthine and 1,N6,ethenoadenine
?
alkylated DNA + H2O
3-methyladenine + ?
-
important role in preventing the mutagenic effects of deaminated purines and cyclic etheno adducts
-
?
alkylated DNA + H2O
3-methyladenine + ?
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calf thymus DNA
prefers to release hypoxanthine and 1,N6,ethenoadenine
?
alkylated DNA + H2O
3-methyladenine + ?
-
important role in preventing the mutagenic effects of deaminated purines and cyclic etheno adducts
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?
alkylated DNA + H2O
3-methyladenine + ?
-
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releases 3-methyladenine and 3-ethyladenine
?
alkylated DNA + H2O
3-methyladenine + ?
-
-
also releases a minmal amount of 3-methylguanine
?
alkylated DNA + H2O
3-methyladenine + ?
-
-
also releases a minmal amount of 3-methylguanine
?
alkylated DNA + H2O
3-methyladenine + ?
smallest member of the helix-hairpin-helix HhH superfamily of DNA glycosylases
-
?
alkylated DNA + H2O
3-methyladenine + ?
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double-stranded DNA is an effective substrate, enzyme is less efficient in excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication
-
?
alkylated DNA + H2O
3-methyladenine + ?
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double-stranded DNA is an effective substrate, enzyme is less efficient in excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
reaction of the enzyme with alkylated DNA leads to introduction of apurinic sites but no chain breaks
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?
alkylated DNA + H2O
3-methyladenine + ?
-
primarily removes N3-methyladenine but also N3-methylguanine from DNA by glycosylic cleavage in the first step of the base excision repair
-
?
alkylated DNA + H2O
3-methyladenine + ?
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constitutive pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine
-
?
alkylated DNA + H2O
3-methyladenine + ?
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constitutive pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
Escherichia coli B / ATCC 11303
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
-
also releases a minmal amount of 3-methylguanine
?
alkylated DNA + H2O
3-methyladenine + ?
-
nuclear transcription factor estrogen receptor alpha interacts with 3-methyladenine DNA glycosylase to modulate transcription and DNA repair, enzyme catalyzes removal of hypoxanthine from DNA, estrogen receptor alpha stabilizes the interaction of the enzyme with hypoxanthine containing DNAand increases the catalytic removal of the modified base
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
50% of DNA alkylation is repaired in the first 60 min after treatment with methyl methanesulfonate
-
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
cellular repair of alkylated DNA base modifications
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?
alkylated DNA + H2O
3-methyladenine + ?
-
constititively expressed
-
?
alkylated DNA + H2O
3-methyladenine + ?
-
-
also removes hypoxanthine and 1,N6-ethenoadenine from DNA, dependent on the structure
?
alkylated DNA + H2O
3-methyladenine + ?
-
also removes 7-methylguanine
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?
alkylated DNA + H2O
3-methyladenine + ?
-
initiates the base excision repair pathway by removing damaged bases to create abasic apurinic/apyrimidinic sites
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?
alkylated DNA + H2O
3-methyladenine + ?
-
highest activity by producing 3-methylguanine
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-
?
alkylated DNA + H2O
3-methyladenine + ?
Saccharomyces pombe
-
highest activity by producing 3-methylguanine
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-
?
alkylated DNA + H2O
7-methylguanine + ?
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50% of DNA alkylation is repaired in the first 60 min after treatment with methyl methanesulfonate
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-
?
additional information
?
-
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AlkC is involved exclusively in the repair of alkylation damage
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-
?
additional information
?
-
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no detectable affinity for hypoxanthine, 8-oxoguanine and 5-formyluracil
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-
?
additional information
?
-
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AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine
-
-
?
additional information
?
-
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will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened
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-
?
additional information
?
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no detectable endonuclease activity on native, depurinated or alkylated plasmid DNA
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-
?
additional information
?
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does not liberate 7-methylguanine, O6-methylguanine, 7-methyladenine, 7-ethylguanine, O6-ethylguanine, and arylalkylated purine derivatives obtained by treatment of DNA with 7-bromomethyl-12-methylbenz[alpha]anthracene, no detectable nuclease activity with native DNA, depurinated DNA, ultraviolet-irradiated DNA, or X-irradiated DNA as potential substrates, enzyme does not release hypoxanthine or xanthine in free form from by nitrous acid treatment partly demainated DNA
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-
?
additional information
?
-
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will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened
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-
?
additional information
?
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3-methyladenine DNA glycosylase recognizes and excises a broad range of purines damaged by alkylation and oxidative damage, including 3-methyladenine, 7-methylguanine, hypoxanthine, and 1,N6-ethenoadenine
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-
?
additional information
?
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both the full-length and truncated AAG excised 1,N2-ethenoguanine, albeit weakly, from duplex DNA, while uracil is excised from both single- and double-stranded DNA, but only by full-length AAG. Structural basis for substrate recognition, base excision, and exclusion of normal purines and pyrimidines from its substrate recognition pocket, mechanism, overview
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-
?
additional information
?
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no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine
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-
?
additional information
?
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AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine
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-
?
additional information
?
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3-methyladenine DNA glycosylase binds 1,N6-ethenoadenine and abasic sites the most tightly, followed by the cross-linked 1,2-d(ApG) cisplatin adduct in duplex DNA but does not catalyze glycosyl bond cleavage at either of the cross-linked bases
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-
?
