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3.2.2.20: DNA-3-methyladenine glycosylase I

This is an abbreviated version!
For detailed information about DNA-3-methyladenine glycosylase I, go to the full flat file.

Word Map on EC 3.2.2.20

Reaction

3-methyladenine-containing DNA
+
H2O
=
3-Methyladenine
 +
deadenylated DNA

Synonyms

3-MeA DNA glycosylase I, 3-methyl adenine DNA glycosylase I, 3-methyladenine DNA glycosylase, 3-methyladenine DNA glycosylase I, 3MeA, 3MeA DNA glycosylase, AAG, Aag 3MeA DNA glycosylase, AlkC, AlkD, APNG, APNG DNA glycosylase, bAag, deoxyribonucleate 3-methyladenine DNA glycosidase I, DNA-3-methyladenine DNA glycosidase I, GI, human m3A DNA glycosylase, m3A DNA glycosylase I, MAG, MAG1, Mag1 3-methyladenine DNA glycosylase, Mag1p, Mag2p, MPG, MpgI, N3-methyladenine DNA glycosylase, protein Tag, TAG, TagA, TagA protein, TagI, Yx1J

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.20 DNA-3-methyladenine glycosylase I

Engineering

Engineering on EC 3.2.2.20 - DNA-3-methyladenine glycosylase I

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D18A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
E38A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
H17A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
W21A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
W46A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Y13A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Y16A
-
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
E121A
-
about 20% of wild-type activity
E156A
-
about 50% of wild-type activity
W164A
-
activity similar to wild-type
D170N
Saccharomyces pombe
-
the mutation substantially decreases enzymatic activity without abolishing it
I147V/T151A/A206S
Saccharomyces pombe
-
the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type
K126N/E130G/N175D
Saccharomyces pombe
-
the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type
L122M
Saccharomyces pombe
-
the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type
N42Y/V165A
Saccharomyces pombe
-
the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type
R149K
Saccharomyces pombe
-
the mutant retains base excision activity toward alkylated DNA substrates, but its level of activity is decreased compared to the wild type
additional information
-
construction of a truncated DELTA80AAG enzyme mutant, substrate specificity in comparison to the wild-type enzyme shows that both the full-length and truncated AAG excise 1,N2-ethenoguanine, albeit weakly, from duplex DNA, while uracil is excised from both single- and double-stranded DNA, but only by full-length AAG, single-turnover excision kinetics, overview