3.2.2.17: deoxyribodipyrimidine endonucleosidase

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For detailed information about deoxyribodipyrimidine endonucleosidase, go to the full flat file.

Reaction

Synonyms

5'dimer DNA glycosylase, bacteriophage T4 endonuclease V, Bsp-pdg, Chlorella virus pyrimidine dimer glycosylase, cis-syn cyclobutane pyrimidine dimer glycosylase, cis-syn trans-syn-II cyclobutane pyrimidine dimer glycosylase, cv-pdg1, denV, deoxyribonucleate pyrimidine dimer glycosidase, deoxyribopyrimidine endonucleosidase, dimer dependent endonuclease V, dimer specific M. luteus endonuclease, dimer-dependent endonuclease, dimericine, endodeoxyribonuclease (apurinic or apyrimidinic), endodeoxyribonuclease (pyrimidine dimer), endonuclease V, glycosylase/abasic (AP) lyase, PD-DNA glycosylase, PD-glycosylase, PD-specific DNA glycosylase, pyrimidine dimer DNA glycosylase, pyrimidine dimer glycosylase, pyrimidine dimer-specific glycosylase/AP lyase, T4 Endo V, T4 endonuclease V, T4 endonuclease V DNA repair enzyme, T4 PD-DNA-glycosylase, T4 Pdg, T4 pyrimidine dimer DNA glycosylase, T4 pyrimidine dimer DNA glycosylase/AP-lyase, T4 pyrimidine dimer glycosylase, T4 UV endonuclease, T4-induced UV endonuclease, T4-PDG, T4PDG, thymine photodimer repair enzyme, UV endonuclease, UV-specific endonuclease

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.2 Hydrolysing N-glycosyl compounds

                3.2.2.17 deoxyribodipyrimidine endonucleosidase

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Results	in table
1	Activating Compound
25	AA Sequence
4	Application
11	Cloned(Commentary)
8	Crystallization (Commentary)
2	General Stability
7	Inhibitors
18	KM Value [mM]
10	Metals/Ions
11	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
45	Organism
2	PDB ID
7	pH Optimum
3	pH Range
13	Protein Variants
21	Purification (Commentary)
4	Reaction
5	Reaction Type
44	Reference
4	Source Tissue
3	Specific Activity [micromol/min/mg]
3	Storage Stability
48	Substrates and Products (Substrate)
5	Subunits
77	Synonyms
1	Systematic Name
2	Temperature Range [°C]
6	Temperature Stability [°C]

Cloned

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Cloned on EC 3.2.2.17 - deoxyribodipyrimidine endonucleosidase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloned and sequenced from 42 Chlorella viruses isolated over 12 years in diverse geographic regions

Paramecium bursaria Chlorella virus 1

-

171812

cloned into a variety of plasmids, express endonuclease V activity in repair-deficient Escherichia coli

Tequatrovirus T4

-

170698

cloned into the pTYB2 vector and overexpressed in Escherichia coli

Paramecium bursaria Chlorella virus 1

-

656903

denVgene cloned and sequenced

Tequatrovirus T4

-

171813

expression in Escherichia coli of several mutants

Escherichia coli

-

656231

gene cv-pdg encoding T4 endonucleaseV homolog, 41% identical to T4endoV, enhance UV radiation resistance in repair-deficient Escherichia coli BL21, vector pCRII, cloning and overexpression of the gene product, cloned into the Escherichia coli expression plasmid pET11a

Paramecium bursaria Chlorella virus 1

-

171809

PCR-amplified from pET11a, subcloned into pTYB2, plasmids subsequently transformed into Escherichia coli ER2566 for expression

Paramecium bursaria Chlorella virus 1

-

171811

PCR-amplified from pUC19, subcloned into pET-11a

Paramecium bursaria Chlorella virus 1

-

171810

pEVC127, cloned and expressed in Escherichia coli HB101, synthetic gene constructed by ligation of oligonucleotide fragments expressed efficiently in Escherichia coli

Tequatrovirus T4

-

170703, 171807

T4 denV (414 bp cDNA) encoding T4 endo V (138 amino acid) is synthesized and expressed using either an expression vector, pTriEx-4, in Escherichia coli or a baculovirus AcNPV vector, pBacPAK8, in insect cells

Tequatrovirus T4

-

681339