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0.49
2'-O-methyluridine
-
pH 7.0, 28°C
0.57
2-aminopurine riboside
0.009
3-deazaadenosine
-
at pH 7.0 and 35°C
0.03
4-nitrophenyl beta-D-ribofuranoside
-
at pH 7.0 and 35°C
0.017 - 0.039
4-nitrophenylriboside
0.078
5'-deoxyadenosine
-
at pH 7.0 and 35°C
1.07 - 13.6
7-methyl guanosine
1.09 - 5.2
7-methylguanosine
6.9
ethenoadenosine
-
at pH 7.0 and 35°C
0.206
p-nitrophenylriboside
pH 7.2, 35°C, His-tagged enzyme form
2 - 3.9
purine ribonucleoside
0.248
purine riboside
-
at pH 7.0 and 35°C
0.57
2-aminopurine riboside
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.57
2-aminopurine riboside
-
at pH 7.0 and 35°C
0.017
4-nitrophenylriboside
mutant C253A, ApH 7.5, 25°C
0.026
4-nitrophenylriboside
mutant C42A, pH 7.5, 25°C
0.039
4-nitrophenylriboside
wild-type, pH 7.5, 25°C
1.07
7-methyl guanosine
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
3.4
7-methyl guanosine
-
wild-type, pH 7.0, 35°C
13.2
7-methyl guanosine
-
mutant R252A, pH 7.0, 35°C
13.6
7-methyl guanosine
-
mutant D40A, pH 7.0, 35°C
1.09
7-methylguanosine
wild-type, pH 7.5, 25°C
1.98
7-methylguanosine
mutant C253A, ApH 7.5, 25°C
2.4
7-methylguanosine
mutant C42A, pH 7.5, 25°C
5.2
7-methylguanosine
-
at pH 7.0 and 5°C
0.0042
adenosine
mutant C253A, ApH 7.5, 25°C
0.023
adenosine
mutant C42A, pH 7.5, 25°C
0.237
adenosine
-
Km less than 0.001 mM, at pH 7.0 and 35°C
0.418
adenosine
wild-type, pH 7.5, 25°C
1.46
adenosine
pH 7.2, 35°C, His-tagged enzyme form
2.1
adenosine
-
pH 7.2, 37°C
2.6
adenosine
-
wild-type, pH 7.0, 35°C
5.7
adenosine
-
mutant R252A, pH 7.0, 35°C
17.3
adenosine
-
recombinant enzyme, pH 7.3, 37°C
0.338
cytidine
pH 7.2, 35°C, His-tagged enzyme form
0.338
cytidine
-
wild-type, pH 7.0, 35°C
0.52
cytidine
-
at pH 7.0 and 35°C
0.54
cytidine
-
mutant R252A, pH 7.0, 35°C
7.8
cytidine
-
pH 7.2, 37°C
0.0051
guanosine
mutant C253A, ApH 7.5, 25°C
0.011
guanosine
mutant C42A, pH 7.5, 25°C
0.143
guanosine
wild-type, pH 7.5, 25°C
1.09
guanosine
mutant enzyme C248P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
1.3
guanosine
-
at pH 7.0 and 5°C
1.4
guanosine
mutant enzyme L250P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
1.7
guanosine
mutant enzyme E249P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
1.9
guanosine
pH 7.2, 35°C, His-tagged enzyme form
2.3
guanosine
-
wild-type, pH 7.0, 35°C
2.3
guanosine
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
2.5
guanosine
mutant enzyme L251P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
2.7
guanosine
-
mutant D40A, pH 7.0, 35°C
3.9
guanosine
mutant enzyme D253P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
4.1
guanosine
mutant enzyme H247P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
9.8
guanosine
-
mutant R252A, pH 7.0, 35°C
13
guanosine
mutant enzyme R252P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
37.3
guanosine
-
recombinant enzyme, pH 7.3, 37°C
37.6
guanosine
-
at pH 7.0 and 35°C
0.0043
Inosine
mutant C253A, ApH 7.5, 25°C
0.024
Inosine
mutant C42A, pH 7.5, 25°C
0.06
Inosine
pH 7.5, 30°C, mutant Y241A
0.203
Inosine
wild-type, pH 7.5, 25°C
0.47
Inosine
pH 7.5, 30°C, mutant Y255A
0.7
Inosine
pH 7.5, 30°C, mutant Y249A
0.85
Inosine
mutant enzyme H79A, pH and temperature not specified in the publication
1.1
Inosine
pH 7.5, 30°C, mutant H245A
1.4
Inosine
pH 7.5, 30°C, mutant Y244A
2 - 8
Inosine
-
at pH 7.0 and 35°C
3
Inosine
pH 7.5, 30°C, mutant D252A
5.19
Inosine
pH 7.2, 35°C, His-tagged enzyme form
5.49
Inosine
pH 7.2, 35°C, wiithout His-tag
5.7
Inosine
pH 7.5, 30°C, mutant E247A
6
Inosine
pH 7.5, 30°C, wild-type enzyme
7.2
Inosine
pH 7.5, 30°C, recombinant enzyme
7.2
Inosine
pH 7.5, 30°C, mutant D250A
8.1
Inosine
-
pH 6.0, 37°C
11.6
Inosine
wild-type enzyme, pH and temperature not specified in the publication
48.9
Inosine
in 50 mM HEPES (pH 7.3), at 25°C
61
Inosine
-
recombinant enzyme, pH 7.3, 37°C
2
purine ribonucleoside
-
mutant R252A, pH 7.0, 35°C
3.9
purine ribonucleoside
-
wild-type, pH 7.0, 35°C
0.0022
uridine
-
-
0.0038
uridine
-
at pH 7.0 and 35°C
0.022
uridine
pH 7.2, 35°C, His-tagged enzyme form
46
uridine
-
pH 7.2, 37°C
0.029
Xanthosine
-
-
0.19
Xanthosine
pH 7.5, 30°C, mutant Y241A
0.43
Xanthosine
pH 7.5, 30°C, mutant Y255A
0.58
Xanthosine
pH 7.5, 30°C, mutant Y244A
1.1
Xanthosine
pH 7.5, 30°C, mutant Y249A
1.3
Xanthosine
pH 7.5, 30°C, mutant H245A
2.5
Xanthosine
pH 7.5, 30°C, mutant D252A
6.2
Xanthosine
pH 7.5, 30°C, recombinant enzyme
6.2
Xanthosine
-
at pH 7.0 and 35°C
7
Xanthosine
pH 7.5, 30°C, wild-type enzyme
7.2
Xanthosine
pH 7.5, 30°C, mutant E247A
8.4
Xanthosine
pH 7.5, 30°C, mutant D250A
26.3
Xanthosine
-
pH 6.0, 37°C
62
Xanthosine
-
pH 7.2, 37°C