3.2.2.1: purine nucleosidase
This is an abbreviated version!
For detailed information about purine nucleosidase, go to the full flat file.
Word Map on EC 3.2.2.1
-
3.2.2.1
-
salvage
-
trypanosoma
-
vivax
-
crithidia
-
fasciculata
-
pyrimidine-specific
-
n-glycosidic
-
iminoribitols
-
parkin
-
schramm
-
formycin
-
horenstein
-
trypanocidal
-
pharmacology
-
drug development
-
analysis
- 3.2.2.1
-
salvage
- trypanosoma
- vivax
-
crithidia
- fasciculata
-
pyrimidine-specific
-
n-glycosidic
- iminoribitols
- parkin
-
schramm
- formycin
-
horenstein
-
trypanocidal
- pharmacology
- drug development
- analysis
Reaction
Synonyms
6-oxopurine nucleosidase, CELE_Y43F8C.13, IAG nucleoside hydrolase, IAG-NH, IAG-nucleoside hydrolase, IAGNH, IG-NH, inosine-adenosine-guanosine nucleoside hydrolase, inosine-adenosine-guanosine-preferring nucleoside hydrolase, inosine-guanosine nucleoside hydrolase, inosine-guanosine-specific nucleoside hydrolase, inosine/adenosine/guanosinepreferring NH, IU-nucleoside hydrolase, IunH, N-ribosyl purine ribohydrolase, N-ribosyl-purine ribohydrolase, NSH3, nucleosidase, nucleosidase g, nucleoside hydrolase, PpNRH1, purine beta-ribosidase, purine hydrolase, purine NRH, Purine nucleosidase, purine nucleoside hydrolase, purine nucleoside N-ribohydrolase, purine ribonucleosidase, purine specific nucleoside hydrolase, purine-preferring nucleoside hydrolase, purine-specific hydrolase, purine-specific inosine-adenosine-guanosine nucleoside hydrolase, purine-specific inosineadenosine-guanosine nucleoside hydrolase, purine-specific NH, purine-specific nucleoside hydrolase, purine-specific ribonucleoside hydrolase, ribonucleoside hydrolase, rih1, rih2, RihC, SsIAG-NH, SslAG-NH, SSO2243, Tb927.3.2960, URH1, URH2, ZmNRH3
ECTree
3.2.2.1 purine nucleosidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.2.1 - purine nucleosidase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-O-methylguanosine + H2O
2-O-methylribose + guanidine
-
-
-
-
?
2'-O-methylinosine + H2O
2-O-methylribose + hypoxanthine
-
-
-
-
?
2,6-diamino-9-beta-D-ribofuranosylpurine + H2O
2,6-diaminopurine + D-ribose
-
76% of the activity with inosine
-
-
ir
2,8-diamino-9-beta-D-ribofuranosyladenine + H2O
2,8-diaminoadenine + D-ribose
-
38% of the activity with inosine
-
-
ir
2-hydroxy-9-beta-D-ribofuranosyladenine + H2O
2-hydroxyadenine + D-ribose
-
10% of the activity with inosine
-
-
ir
3'-AMP + H2O
adenine + D-ribose 3-phosphate
E15229
0.67% of the activity with adenosine
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + beta-D-ribofuranose
-
-
-
-
?
5,6-dimethyl-1-beta-D-ribofuranosylbenzimidazole + H2O
5,6-dimethylbenzimidazole + D-ribose
-
200% of the activity with inosine
-
-
ir
6-dimethylamino-9-beta-D-ribofuranosylbenzimidazole + H2O
6-dimethylaminobenzimidazole + D-ribose
-
30% of the activity with inosine
-
-
ir
6-mercaptopurine ribonucleoside + H2O
6-mercaptopurine + ribose
-
-
-
-
?
9-beta-D-ribofuranosylpurine + H2O
purine + D-ribose
-
47% of the activity with inosine
-
-
ir
imidazoleacetic acid riboside + H2O
imidazoleacetate + D-ribose
-
5% of the activity with inosine
-
-
ir
nicotinamide riboside + H2O
nicotinamide + D-ribose
-
150% of the activity with inosine
-
-
ir
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + D-ribose
-
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + D-ribose
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
15% of the activity with inosine
-
-
?
adenosine + H2O
D-ribose + adenine
-
103% of the activity with inosine
-
-
ir
adenosine + H2O
D-ribose + adenine
-
IAG-NH shows higher affinity for adenosine with respect to inosine and guanosine, but the catalytic efficiency of these substrates is comparable
-
-
?
adenosine + H2O
D-ribose + adenine
-
substrate for inosine-adenosine-guanosine nucleoside hydrolase
-
-
?
adenosine + H2O
D-ribose + adenine
-
3.6% of the activity with xanthosine
-
-
?
cytidine + H2O
cytosine + D-ribose
-
about 20% of the rate with adenosine
-
-
?
cytidine + H2O
D-ribose + cytosine
-
54% of the activity with inosine
-
-
ir
guanosine + H2O
D-ribose + guanine
-
33% of the activity with inosine
-
-
?
guanosine + H2O
D-ribose + guanine
-
about 80% of the rate with adenosine
-
-
?
guanosine + H2O
D-ribose + guanine
-
90% of the activity with inosine
-
-
ir
guanosine + H2O
D-ribose + guanine
-
1.4% of the activity with xanthosine
-
-
?
guanosine + H2O
guanine + D-ribose
E15229
29.5% of the activity with adenosine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
87% of the activity with xanthosine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
Physcomitrium patens Gransden 2004
87% of the activity with xanthosine
-
-
?
inosine + H2O
hypoxanthine + D-ribose
E15229
78.9% of the activity with adenosine
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
kinetic mechanism is rapid equilibrium with random product release
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
rate of hydrolysis similar to adenosine
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
kinetic mechanism is rapid equilibrium with random product release
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
25% of the activity with xanthosine
-
-
?
uridine + H2O
D-ribose + uracil
-
substrate for isoform URH1, but nor URH2
-
-
?
uridine + H2O
D-ribose + uracil
-
about 15% of the rate with adenosine
-
-
?
xanthosine + H2O
D-ribose + xanthine
Physcomitrium patens Gransden 2004
best substrate
-
-
?
xanthosine + H2O
xanthine + D-ribose
E15229
63.5% of the activity with adenosine
-
-
?
additional information
?
-
-
no hydrolysis of the pyrimidines uridine and cytidine
-
-
?
additional information
?
-
hydrolase activity could not be detected when uridine or cytidine is used as a substrate
-
-
?
additional information
?
-
-
hydrolase activity could not be detected when uridine or cytidine is used as a substrate
-
-
?
additional information
?
-
IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine
-
-
?
additional information
?
-
-
IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine
-
-
?
additional information
?
-
-
isoform rih1 shows pyrimidine-specific ribonuclease activity. Activity of isoform rih2 in decreasing order: inosine, adenine, uridine, guanosine, xanthosine, cytidine
-
-
?
additional information
?
-
-
the enzyme prefers ribonucleosides and 2'-O-methylribonucleosides as its hydrolytic substrates, but reacts weakly with 2'-deoxyribonucleosides
-
-
?
additional information
?
-
poor activity with adenosine, uridine, and guanosine, no activity with cytokinin riboside, cytidine, and trans-zeatin riboside. Substrate docking into the active site, overview
-
-
?
additional information
?
-
-
poor activity with adenosine, uridine, and guanosine, no activity with cytokinin riboside, cytidine, and trans-zeatin riboside. Substrate docking into the active site, overview
-
-
?
additional information
?
-
Physcomitrium patens Gransden 2004
poor activity with adenosine, uridine, and guanosine, no activity with cytokinin riboside, cytidine, and trans-zeatin riboside. Substrate docking into the active site, overview
-
-
?
additional information
?
-
-
IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+
-
-
?
additional information
?
-
the enzyme catalyzes the irreversible hydrolysis of the N-glycosidic bond of beta-ribonucleosides forming ribose and purine base, substrate specificity and involved residues, overview
-
-
?
additional information
?
-
-
IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+
-
-
?
additional information
?
-
-
cytidine, uridine, and 4-nitrophenyl-riboside are poor substrates
-
-
?
additional information
?
-
-
inosine-guanosine nucleoside hydrolase does not use adenosine as substrate
-
-
?
additional information
?
-
-
cytidine, uridine, and 4-nitrophenyl-riboside are poor substrates
-
-
?
additional information
?
-
poor activity with adenosine, uridine, guanosine, trans-zeatin riboside, and cytokinin riboside, almost no activity with cytidine. Substrate docking into the active site, overview
-
-
?
