3.2.2.1: purine nucleosidase

This is an abbreviated version!
For detailed information about purine nucleosidase, go to the full flat file.

Word Map on EC 3.2.2.1

3.2.2.1

salvage

trypanosoma

vivax

crithidia

fasciculata

pyrimidine-specific

n-glycosidic

iminoribitols

parkin

schramm

formycin

horenstein

trypanocidal

pharmacology

drug development

analysis

Reaction

Synonyms

6-oxopurine nucleosidase, CELE_Y43F8C.13, IAG nucleoside hydrolase, IAG-NH, IAG-nucleoside hydrolase, IAGNH, IG-NH, inosine-adenosine-guanosine nucleoside hydrolase, inosine-adenosine-guanosine-preferring nucleoside hydrolase, inosine-guanosine nucleoside hydrolase, inosine-guanosine-specific nucleoside hydrolase, inosine/adenosine/guanosinepreferring NH, IU-nucleoside hydrolase, IunH, N-ribosyl purine ribohydrolase, N-ribosyl-purine ribohydrolase, NSH3, nucleosidase, nucleosidase g, nucleoside hydrolase, PpNRH1, purine beta-ribosidase, purine hydrolase, purine NRH, Purine nucleosidase, purine nucleoside hydrolase, purine nucleoside N-ribohydrolase, purine ribonucleosidase, purine specific nucleoside hydrolase, purine-preferring nucleoside hydrolase, purine-specific hydrolase, purine-specific inosine-adenosine-guanosine nucleoside hydrolase, purine-specific inosineadenosine-guanosine nucleoside hydrolase, purine-specific NH, purine-specific nucleoside hydrolase, purine-specific ribonucleoside hydrolase, ribonucleoside hydrolase, rih1, rih2, RihC, SsIAG-NH, SslAG-NH, SSO2243, Tb927.3.2960, URH1, URH2, ZmNRH3

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.2 Hydrolysing N-glycosyl compounds

3.2.2.1 purine nucleosidase

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Results	in table
2	Activating Compound
1462	AA Sequence
5	Application
1	CAS Registry Number
20	Cloned(Commentary)
14	Crystallization (Commentary)
10	Disease/ Diagnostics
11	General Information
6	General Stability
2	IC50 Value
192	Inhibitors
53	kcat/KM [mM/s]
131	Ki Value [mM]
138	KM Value [mM]
4	Localization
9	Metals/Ions
25	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
122	Organism
9	Pathway
79	PDB ID
19	pH Optimum
5	pH Range
12	pH Stability
2	pI Value
32	Protein Variants
25	Purification (Commentary)
4	Reaction
2	Reaction Type
80	Reference
32	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
222	Substrates and Products (Substrate)
15	Subunits
86	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
5	Temperature Range [°C]
17	Temperature Stability [°C]
103	Turnover Number [1/s]

Purification

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Purification on EC 3.2.2.1 - purine nucleosidase

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12 entries

methylthioinosine-Sepharose column chromatography

Saccharolobus solfataricus

695873

Ni-NTA agarose column chromatography

Arabidopsis thaliana

716358

Ni-NTA column chromatography

Bacillus thuringiensis

A7UHH1

700035

Ni-NTA column chromatography and Superdex-200 gel filtration

2 entries

Ni-NTA column chromatography and Superdex-75 gel filtration

Trypanosoma vivax

Q9GPQ4

698781

Ni-NTA resin column chromatography and Superdex 200 gel filtration

Trypanosoma brucei brucei

714225

recombinant His-tagged wild-type and mutant enzymes by cobalt affinity chromatography and gel filtration

2 entries

recombinant mutant enzyme L221Y/N228V from Escherichia coli

Saccharolobus solfataricus

Q97WH6

731139

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by gel filtration

Trypanosoma brucei

731054

recombinant protein

Lentilactobacillus buchneri

750094