3.2.1.B35: Thermococcus kodakaraensis beta-glycosidase
This is an abbreviated version!
For detailed information about Thermococcus kodakaraensis beta-glycosidase, go to the full flat file.
Reaction
Exo-type beta-glycosidase activity with cellobiose, cellotriose, cellotetraose or cellopentaose. In vitro it shows high catalytic efficiency with 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-mannopyranoside, and lower catalytic efficiency with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-xylopyranoside =
Synonyms
Pyrococcus kodakaraensis beta-glycosidase
ECTree
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KM Value
KM Value on EC 3.2.1.B35 - Thermococcus kodakaraensis beta-glycosidase
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0.94
80°C, pH not specified in the publication, wild-type enzyme
1.29
4-nitrophenyl beta-D-fucopyranoside
80°C, pH not specified in the publication, mutant enzyme D206N
4.6
4-nitrophenyl beta-D-glucopyranoside
80°C, pH not specified in the publication, wild-type enzyme
9.23
4-nitrophenyl beta-D-glucopyranoside
80°C, pH not specified in the publication, mutant enzyme D206N
0.4
4-nitrophenyl beta-D-mannopyranoside
80°C, pH not specified in the publication, mutant enzyme D206N
1.08
4-nitrophenyl beta-D-mannopyranoside
80°C, pH not specified in the publication, wild-type enzyme